| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAC2 |
| Uniprot No | P15153 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-189 aa |
| 活性数据 | MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDSKP VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASY ENVRAKWFPE VRHHCPSTPI ILVGTKLDLR DDKDTIEKLK EKKLAPITYP QGLALAKEID SVKYLECSAL TQRGLKTVFD EAIRAVLCPQ PTRQQKRAC |
| 分子量 | 21.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAC2蛋白的3篇参考文献的简要总结(信息为虚构示例，实际文献请查询数据库)：
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1. **文献名称**: *RAC2 GTPase deficiency alters neutrophil motility and immune function*
**作者**: Hanna, S. et al. (2015)
**摘要**: 研究报道了RAC2重组蛋白在体外激活实验中的功能，发现RAC2缺陷会导致中性粒细胞迁移障碍和活性氧(ROS)生成减少，揭示其在小GTP酶调控免疫反应中的关键作用。
2. **文献名称**: *Recombinant RAC2 expression and its role in phagocytosis signaling*
**作者**: Gu, Y. et al. (2018)
**摘要**: 描述了通过大肠杆菌系统表达并纯化重组RAC2蛋白，实验证实其参与巨噬细胞吞噬过程的信号传导，并揭示其与PI3K通路的相互作用机制。
3. **文献名称**: *Structural insights into RAC2 activation by T-cell receptors*
**作者**: Singh, R.K. et al. (2020)
**摘要**: 通过晶体结构解析重组RAC2蛋白与GEF因子(Vav1)的复合物，阐明了其在T细胞受体信号中激活的分子基础，为免疫缺陷疾病治疗提供理论依据。
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**提示**：真实文献建议通过PubMed或Google Scholar检索关键词“recombinant RAC2 protein”“RAC2 GTPase function”等获取最新研究。
RAC2 (Ras-related C3 botulinum toxin substrate 2) is a small GTPase belonging to the Rho family, which plays a pivotal role in regulating intracellular signaling pathways. Expressed predominantly in hematopoietic cells, RAC2 is essential for processes like cell migration, phagocytosis, superoxide production, and cytoskeletal reorganization. It cycles between an inactive GDP-bound state and an active GTP-bound state, controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Dysregulation of RAC2 is linked to immune disorders; for instance, mutations cause severe neutrophil dysfunction, chronic granulomatous disease, or combined immunodeficiency.
Recombinant human RAC2 protein is generated via expression systems (e.g., E. coli, mammalian cells) for structural and functional studies. It aids in exploring RAC2's interaction with effectors (e.g., PAK1. NADPH oxidase) and its role in immune response modulation. Research also focuses on its therapeutic potential, such as targeting RAC2-driven pathways in inflammation or cancer. Notably, RAC2 differs from ubiquitously expressed RAC1 in cell-type specificity and functional redundancy, making it a unique subject for understanding immune cell biology. Studies using recombinant RAC2 help dissect its isoform-specific roles and develop precision therapies for hematologic and autoimmune diseases.
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