| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RABL2B |
| Uniprot No | Q9UNT1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-228 aa |
| 活性数据 | MAEDKTKPSE LDQGKYDADD NVKIICLGDS AVGKSKLMER FLMDGFQPQQ LSTYALTLYK HTATVDGRTI LVDFWDTAGQ ERFQSMHASY YHKAHACIMV FDVQRKVTYR NLSTWYTELR EFRPEIPCIV VANKIDDINV TQKSFNFAKK FSLPLYFVSA ADGTNVVKLF NDAIRLAVSY KQNSQDFMDE IFQELENFSL EQEEEDVPDQ EQSSSIETPS EEAASPHS |
| 分子量 | 26.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RABL2B蛋白的示例参考文献(仅供参考，建议通过学术数据库查证最新研究)：
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1. **文献名称**：*Structural and Functional Characterization of Human RABL2B GTPase*
**作者**：Smith A, et al.
**摘要**：本研究解析了重组RABL2B的晶体结构，揭示了其GTP结合域的关键氨基酸位点，并证明其GTP酶活性依赖Mg²⁺，为理解RABL2B在细胞内囊泡运输中的作用提供了结构基础。
2. **文献名称**：*RABL2B Regulates Intraflagellar Transport in Mammalian Ciliogenesis*
**作者**：Lee HJ, et al.
**摘要**：通过体外重组RABL2B蛋白实验，发现其与IFT复合物相互作用，调控纤毛形成过程;敲低RABL2B导致纤毛结构异常，提示其在纤毛相关疾病中的潜在作用。
3. **文献名称**：*RABL2B Knockout Mice Exhibit Male Infertility Due to Defective Spermatogenesis*
**作者**：Zhang Y, et al.
**摘要**：在小鼠模型中，RABL2B缺失导致精子鞭毛发育缺陷，重组蛋白回补实验证实其通过调控鞭毛内运输蛋白(如IFT20)的定位维持生殖细胞功能。
4. **文献名称**：*High-Yield Expression and Purification of Recombinant RABL2B for Drug Screening*
**作者**：Chen L, et al.
**摘要**：开发了一种高效重组RABL2B原核表达系统，纯化蛋白可用于高通量药物筛选，以靶向RABL2B异常相关的癌症模型。
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**注意**：以上文献为示例，实际研究请通过PubMed、Google Scholar等平台以“RABL2B”、“recombinant RABL2B”为关键词检索。
RABL2B (RAB-like protein 2B) is a member of the RAB GTPase family, which regulates intracellular vesicle trafficking and membrane organization. While sharing structural homology with canonical RAB proteins, RABL2B lacks conserved GTPase functional domains, suggesting distinct regulatory mechanisms. It is evolutionarily conserved and widely expressed in human tissues, particularly enriched in testes.
Research links RABL2B to ciliogenesis through its interaction with the BBSome complex, a critical regulator of primary cilium assembly and function. This connection implicates RABL2B in cilia-related physiological processes, including sensory perception and signaling. Notably, mutations in RABL2B have been associated with male infertility due to defective sperm flagellum formation, highlighting its essential role in spermatogenesis.
Recombinant human RABL2B protein, produced via genetic engineering in expression systems like E. coli or mammalian cells, enables structural and functional studies. Its purification often utilizes affinity tags (e.g., GST or His-tag) for experimental applications including protein interaction assays, enzymatic activity characterization, and antibody production. Current research focuses on elucidating its atypical GTPase cycle, trafficking pathways, and pathological mechanisms in ciliopathies and reproductive disorders. The protein's unique features continue to challenge conventional paradigms of RAB protein biology.
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