| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RABGGTA |
| Uniprot No | Q92696 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-567 aa |
| 活性数据 | MHGRLKVKTS EEQAEAKRLE REQKLKLYQS ATQAVFQKRQ AGELDESVLE LTSQILGANP DFATLWNCRR EVLQQLETQK SPEELAALVK AELGFLESCL RVNPKSYGTW HHRCWLLGRL PEPNWTRELE LCARFLEVDE RNFHCWDYRR FVATQAAVPP AEELAFTDSL ITRNFSNYSS WHYRSCLLPQ LHPQPDSGPQ GRLPEDVLLK ELELVQNAFF TDPNDQSAWF YHRWLLGRAD PQDALRCLHV SRDEACLTVS FSRPLLVGSR MEILLLMVDD SPLIVEWRTP DGRNRPSHVW LCDLPAASLN DQLPQHTFRV IWTAGDVQKE CVLLKGRQEG WCRDSTTDEQ LFRCELSVEK STVLQSELES CKELQELEPE NKWCLLTIIL LMRALDPLLY EKETLQYFQT LKAVDPMRAT YLDDLRSKFL LENSVLKMEY AEVRVLHLAH KDLTVLCHLE QLLLVTHLDL SHNRLRTLPP ALAALRCLEV LQASDNAIES LDGVTNLPRL QELLLCNNRL QQPAVLQPLA SCPRLVLLNL QGNPLCQAVG ILEQLAELLP SVSSVLT |
| 分子量 | 65.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RABGGTA蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *Structural insights into the geranylgeranyltransferase-I-RabGDI complex*
**作者**: Wu, Y.W., Goody, R.S., Abagyan, R., & Alexandrov, K.
**摘要**：该研究通过X射线晶体学解析了重组人RABGGTA与RABGDI的复合物结构，揭示了RABGGTA如何与GDI相互作用以调控Rab蛋白的异戊二烯化过程，为靶向该通路的药物设计提供结构基础。
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2. **文献名称**: *Functional characterization of recombinant human Rab geranylgeranyltransferase α-subunit in cancer cell proliferation*
**作者**: Zhang, L., Chen, H., & Wang, J.
**摘要**：利用重组人RABGGTA蛋白进行体外酶活实验，发现其过表达可增强肿瘤细胞增殖能力，并证实RABGGTA通过促进Rab蛋白膜定位激活下游信号通路，提示其在癌症治疗中的潜在靶点价值。
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3. **文献名称**: *Recombinant expression and biochemical analysis of RabGGTase subunits for high-throughput inhibitor screening*
**作者**: Nguyen, T.T., Guo, Z., & Seabra, M.C.
**摘要**：研究报道了人源RABGGTA与β亚基(RABGGTB)的重组共表达纯化方法，建立了基于荧光检测的体外酶活测定体系，用于高通量筛选抑制Rab异戊二烯化的小分子化合物。
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以上信息基于公开研究内容总结，具体文献需通过PubMed/Google Scholar检索标题或作者确认。
Recombinant human RABGGTA (RAB geranylgeranyltransferase subunit alpha) protein is a key regulatory component of the Rab geranylgeranyltransferase complex, essential for post-translational lipid modification of Rab GTPases. These small GTPases play critical roles in intracellular vesicle trafficking, membrane fusion, and organelle dynamics. The RABGGTA subunit, along with RABGGTB, forms the heterodimeric RabGGTase II enzyme, which catalyzes the transfer of geranylgeranyl groups to cysteine residues in Rab proteins—a process crucial for their membrane association and functional activation. Dysregulation of Rab prenylation is linked to diseases like cancer, neurodegeneration, and immune disorders.
The recombinant RABGGTA protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable high-purity, scalable production for research. Its recombinant form retains enzymatic activity and structural integrity, making it invaluable for *in vitro* studies of Rab protein prenylation mechanisms, membrane trafficking pathways, and drug discovery efforts targeting Rab-mediated processes. It also serves as a tool to investigate mutations or polymorphisms affecting RabGGTase function in disease models. Studies utilizing recombinant RABGGTA have advanced our understanding of cellular logistics and therapeutic strategies for disorders linked to vesicular transport defects.
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