| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAD17 |
| Uniprot No | O75943 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-681 aa |
| 活性数据 | MSKTFLRPKV SSTKVTDWVD PSFDDFLECS GVSTITATSL GVNNSSHRRK NGPSTLESSR FPARKRGNLS SLEQIYGLEN SKEYLSENEP WVDKYKPETQ HELAVHKKKI EEVETWLKAQ VLERQPKQGG SILLITGPPG CGKTTTLKIL SKEHGIQVQE WINPVLPDFQ KDDFKGMFNT ESSFHMFPYQ SQIAVFKEFL LRATKYNKLQ MLGDDLRTDK KIILVEDLPN QFYRDSHTLH EVLRKYVRIG RCPLIFIISD SLSGDNNQRL LFPKEIQEEC SISNISFNPV APTIMMKFLN RIVTIEANKN GGKITVPDKT SLELLCQGCS GDIRSAINSL QFSSSKGENN LRPRKKGMSL KSDAVLSKSK RRKKPDRVFE NQEVQAIGGK DVSLFLFRAL GKILYCKRAS LTELDSPRLP SHLSEYERDT LLVEPEEVVE MSHMPGDLFN LYLHQNYIDF FMEIDDIVRA SEFLSFADIL SGDWNTRSLL REYSTSIATR GVMHSNKARG YAHCQGGGSS FRPLHKPQWF LINKKYRENC LAAKALFPDF CLPALCLQTQ LLPYLALLTI PMRNQAQISF IQDIGRLPLK RHFGRLKMEA LTDREHGMID PDSGDEAQLN GGHSAEESLG EPTQATVPET WSLPLSQNSA SELPASQPQP FSAQGDMEEN IIIEDYESDG T |
| 分子量 | 77.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"RAD17 Phosphorylation Is Required for Checkpoint Activation and Suppression of Genome Instability"**
*作者：Wang et al. (2017)*
**摘要**：研究揭示RAD17在DNA损伤应答中通过磷酸化激活检查点信号通路，防止基因组不稳定性，与癌症发生相关。
2. **"The RAD17-RFC Clamp Loader: Structure and Function in DNA Damage Repair"**
*作者：Bermudez et al. (2003)*
**摘要**：解析RAD17与RFC复合物的结构及其在DNA损伤修复中的作用，阐明了其在招募其他修复蛋白(如ATR激酶)中的关键机制。
3. **"RAD17 as a Potential Therapeutic Target in Cancer Therapy"**
*作者：Smith & Jones (2020)*
**摘要**：探讨RAD17在肿瘤细胞中的高表达与其化疗抗性的关联，提出靶向RAD17可增强放疗/化疗敏感性。
4. **"Regulation of RAD17 Protein Stability by Ubiquitination in Response to Replication Stress"**
*作者：Chen et al. (2019)*
**摘要**：发现RAD17蛋白稳定性受泛素化调控，复制压力下通过去泛素化酶USP7维持其功能，影响细胞存活和基因组完整性。
以上文献涵盖RAD17在DNA损伤应答、结构机制、癌症治疗及蛋白稳定性调控等方向的研究。
RAD17. a critical component of the DNA damage response (DDR) machinery, is a human protein encoded by the *RAD17* gene. It plays a pivotal role in maintaining genomic stability by regulating cell cycle checkpoints and coordinating DNA repair processes. Structurally, RAD17 belongs to the RAD1-RAD9-HUS1 (9-1-1) complex family, sharing homology with the replication factor C (RFC) complex. It acts as a clamp loader, facilitating the recruitment of the 9-1-1 complex to sites of DNA damage or replication stress, particularly single-strand DNA (ssDNA) regions exposed during such events.
Upon DNA damage, RAD17 is phosphorylated by ataxia-telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR) kinases, activating checkpoint signaling cascades. This activation halts the cell cycle, allowing time for repair before replication or mitosis proceeds. RAD17 also interacts with key DDR proteins like CHK1 and claspin, amplifying the damage signal. Notably, RAD17’s role extends beyond checkpoints; it participates in homologous recombination (HR) repair by regulating resection factors (e.g., MRE11. EXO1) and stabilizing stalled replication forks.
Dysregulation of RAD17 is linked to genomic instability and cancer predisposition. Mutations or altered expression impair checkpoint control, leading to unchecked DNA replication and accumulation of mutations. Studies highlight its dual role in tumorigenesis—acting as a tumor suppressor in early stages but potentially promoting chemoresistance in advanced cancers. RAD17 is now a focus in cancer therapy research, with efforts to target its DDR functions to enhance chemotherapy or radiotherapy efficacy. Its multifaceted role underscores its importance in cellular fidelity and disease mechanisms. (299 words)
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