| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAD21 |
| Uniprot No | O60216 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-631 aa |
| 活性数据 | MFYAHFVLSK RGPLAKIWLA AHWDKKLTKA HVFECNLESS VESIISPKVK MALRTSGHLL LGVVRIYHRK AKYLLADCNE AFIKIKMAFR PGVVDLPEEN REAAYNAITL PEEFHDFDQP LPDLDDIDVA QQFSLNQSRV EEITMREEVG NISILQENDF GDFGMDDREI MREGSAFEDD DMLVSTTTSN LLLESEQSTS NLNEKINHLE YEDQYKDDNF GEGNDGGILD DKLISNNDGG IFDDPPALSE AGVMLPEQPA HDDMDEDDNV SMGGPDSPDS VDPVEPMPTM TDQTTLVPNE EEAFALEPID ITVKETKAKR KRKLIVDSVK ELDSKTIRAQ LSDYSDIVTT LDLAPPTKKL MMWKETGGVE KLFSLPAQPL WNNRLLKLFT RCLTPLVPED LRKRRKGGEA DNLDEFLKEF ENPEVPREDQ QQQHQQRDVI DEPIIEEPSR LQESVMEASR TNIDESAMPP PPPQGVKRKA GQIDPEPVMP PQQVEQMEIP PVELPPEEPP NICQLIPELE LLPEKEKEKE KEKEDDEEEE DEDASGGDQD QEERRWNKRT QQMLHGLQRA LAKTGAESIS LLELCRNTNR KQAAAKFYSF LVLKKQQAIE LTQEEPYSDI IATPGPRFHI I |
| 分子量 | 71.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAD21蛋白的3篇文献概览(基于公开信息模拟，非真实文献)：
1. **文献名称**：*Crystal structure of the human cohesin subunit RAD21*
**作者**：Anderson DE, et al.
**摘要**：通过X射线晶体学解析了人RAD21蛋白的核结构，揭示了其与SMC1/SMC3亚基结合的界面，阐明了其在黏连蛋白复合体中维持染色体稳定的分子机制。
2. **文献名称**：*RAD21 is essential for mitotic progression and sister chromatid cohesion*
**作者**：Horsfield JA, et al.
**摘要**：利用基因敲除小鼠模型证明，RAD21缺失导致胚胎致死性，并引发有丝分裂中姐妹染色体分离异常，证实其在染色体正确分配中的必要性。
3. **文献名称**：*RAD21 mutations drive genomic instability in breast cancer*
**作者**：Xu H, et al.
**摘要**：在乳腺癌样本中发现RAD21基因高频突变，其异常表达与染色体非整倍性及患者不良预后显著相关，提示其作为肿瘤抑制因子的潜在作用。
如需具体文献，建议通过PubMed或Web of Science以“RAD21 cohesin”等关键词检索近期高被引论文。
RAD21 (double-strand-break repair protein rad21 homolog) is a crucial component of the cohesin complex, evolutionarily conserved across eukaryotes. This multi-subunit complex, comprising RAD21. SMC1A, SMC3. and STAG1/2. mediates sister chromatid cohesion during mitosis/meiosis, ensuring accurate chromosome segregation. RAD21 bridges SMC1A and SMC3. forming a ring-like structure that entraps sister DNA strands. Beyond mechanical cohesion, it participates in DNA repair, chromatin organization, and transcriptional regulation.
Cell cycle-regulated proteolysis controls RAD21 activity: separase cleaves RAD21 during anaphase to release chromatids. Dysregulation of this process can lead to aneuploidy or genomic instability. Mutations or aberrant expression of RAD21 are linked to developmental disorders like Cornelia de Lange syndrome (CdLS) and cancers. In CdLS, pathogenic variants disrupt cohesin’s transcriptional regulatory roles, causing growth and cognitive deficits. In oncology, RAD21 exhibits dual roles—acting as an oncogene in breast/colorectal cancers by promoting proliferation, yet as a tumor suppressor in hematologic malignancies through genome stability maintenance.
RAD21’s involvement in 3D chromatin architecture underscores its non-canonical functions in gene enhancer-promoter interactions. Isoforms (e.g., RAD21L in germ cells) exhibit tissue-specific roles, influencing meiosis. Research leverages knockout models, structural biology (cryo-EM), and small-molecule inhibitors to explore therapeutic targeting. Despite functional complexity, RAD21 remains central to understanding chromosomal dynamics and disease pathogenesis.
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