| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAD51L1 |
| Uniprot No | O15315 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-384 aa |
| 活性数据 | MGSKKLKRVG LSQELCDRLS RHQILTCQDF LCLSPLELMK VTGLSYRGVH ELLCMVSRAC APKMQTAYGI KAQRSADFSP AFLSTTLSAL DEALHGGVAC GSLTEITGPP GCGKTQFCIM MSILATLPTN MGGLEGAVVY IDTESAFSAE RLVEIAESRF PRYFNTEEKL LLTSSKVHLY RELTCDEVLQ RIESLEEEII SKGIKLVILD SVASVVRKEF DAQLQGNLKE RNKFLAREAS SLKYLAEEFS IPVILTNQIT THLSGALASQ ADLVSPADDL SLSEGTSGSS CVIAALGNTW SHSVNTRLIL QYLDSERRQI LIAKSPLAPF TSFVYTIKEE GLVLQETTFC SVTQAELNWA PEILPPQPPE QLGLQMCHHT QLIF |
| 分子量 | 42.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAD51L1蛋白的3篇参考文献概述：
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1. **文献名称**：*RAD51L1 (RAD51B) is essential for homologous recombination and genomic stability*
**作者**：Takata, M. et al.
**摘要**：该研究通过基因敲除实验，揭示了RAD51L1在哺乳动物细胞同源重组修复中的关键作用，其缺失导致染色体断裂、姐妹染色单体交换异常，并显著增加基因组不稳定性。
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2. **文献名称**：*Structure and functional analysis of the RAD51L1-RAD51C DNA repair complex*
**作者**：Cartwright, R. et al.
**摘要**：通过X射线晶体学解析了RAD51L1与RAD51C形成的复合体三维结构，阐明了两者在DNA结合与重组酶活性中的协同机制，为理解同源重组修复的分子机制提供结构基础。
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3. **文献名称**：*Germline mutations in RAD51L1 confer susceptibility to ovarian and breast cancers*
**作者**：Meindl, A. et al.
**摘要**：通过对家族性乳腺癌/卵巢癌患者的基因测序，发现RAD51L1的遗传突变可能导致DNA修复功能缺陷，显著提高癌症易感性，提示其作为生物标志物的潜在价值。
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额外补充：
4. **文献名称**：*RAD51L1 interacts with XRCC2 to facilitate Holliday junction resolution*
**作者**：Brennan, L. et al.
**摘要**：通过生化实验证实RAD51L1与XRCC2形成复合体，协同促进Holliday junction(DNA重组中间体)的解离，揭示其在修复后期步骤中的调控机制。
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**备注**：以上为根据领域知识的示例性文献框架，实际文献需通过PubMed(PMID)、Web of Science或Google Scholar检索验证。建议使用关键词“RAD51L1/RAD51B”配合“DNA repair”“cancer”“complex structure”进行精准筛选。
**Background of RAD51L1 (RAD51 Homolog L1) Protein**
RAD51L1 (RAD51 homolog L1), also known as RAD51B, is a critical component of the RAD51 family, which plays a central role in homologous recombination repair (HRR) of DNA double-strand breaks (DSBs). As one of five vertebrate paralogs of RAD51 (RAD51. RAD51B, RAD51C, RAD51D, XRCC2. XRCC3), RAD51L1 forms heterodimeric complexes with other paralogs (e.g., RAD51C-RAD51L1 in the BCDX2 complex) to facilitate HRR. These complexes stabilize stalled replication forks, promote strand pairing, and ensure accurate repair by homologous recombination, thereby maintaining genomic stability.
Structurally, RAD51L1 contains conserved Walker A/B nucleotide-binding motifs, enabling ATPase activity critical for its function. It interacts with key HRR mediators like BRCA2 and coordinates with RAD51 filaments during strand invasion. Dysregulation or mutations in RAD51L1 are linked to genomic instability, cancer predisposition (e.g., breast, ovarian cancers), and developmental disorders. Its aberrant expression has been implicated in chemoresistance, making it a potential biomarker or therapeutic target.
Evolutionarily conserved across eukaryotes, RAD51L1 highlights the essentiality of HRR in preserving DNA integrity. Studies continue to explore its regulatory mechanisms, post-translational modifications, and crosstalk with other DNA repair pathways to uncover its broader roles in cellular homeostasis and disease.
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