| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAD54L |
| Uniprot No | Q92698 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-747 aa |
| 活性数据 | MRRSLAPSQL AKRKPEGRSC DDEDWQPGLV TPRKRKSSSE TQIQECFLSP FRKPLSQLTN QPPCLDSSQH EAFIRSILSK PFKVPIPNYQ GPLGSRALGL KRAGVRRALH DPLEKDALVL YEPPPLSAHD QLKLDKEKLP VHVVVDPILS KVLRPHQREG VKFLWECVTS RRIPGSHGCI MADEMGLGKT LQCITLMWTL LRQSPECKPE IDKAVVVSPS SLVKNWYNEV GKWLGGRIQP LAIDGGSKDE IDQKLEGFMN QRGARVSSPI LIISYETFRL HVGVLQKGSV GLVICDEGHR LKNSENQTYQ ALDSLNTSRR VLISGTPIQN DLLEYFSLVH FVNSGILGTA HEFKKHFELP ILKGRDAAAS EADRQLGEER LRELTSIVNR CLIRRTSDIL SKYLPVKIEQ VVCCRLTPLQ TELYKRFLRQ AKPAEELLEG KMSVSSLSSI TSLKKLCNHP ALIYDKCVEE EDGFVGALDL FPPGYSSKAL EPQLSGKMLV LDYILAVTRS RSSDKVVLVS NYTQTLDLFE KLCRARRYLY VRLDGTMSIK KRAKVVERFN SPSSPDFVFM LSSKAGGCGL NLIGANRLVM FDPDWNPAND EQAMARVWRD GQKKTCYIYR LLSAGTIEEK IFQRQSHKKA LSSCVVDEEQ DVERHFSLGE LKELFILDEA SLSDTHDRLH CRRCVNSRQI RPPPDGSDCT SDLAGWNHCT DKWGLRDEVL QAAWDAASTA ITFVFHQRSH EEQRGLR |
| 分子量 | 84.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAD54L蛋白的参考文献示例(注：文献信息为模拟整理，建议通过学术数据库核查准确性)：
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1. **文献名称**: *"RAD54L promotes homologous recombination by enhancing RAD51-mediated DNA strand exchange"*
**作者**: Petukhova, G., Sung, P., & Klein, H.
**摘要**: 研究利用重组人RAD54L蛋白，揭示了其通过结合DNA并刺激RAD51的ATP酶活性，促进同源配对和链交换的分子机制，支持其在同源重组修复中的核心作用。
2. **文献名称**: *"Structural insights into RAD54L’s role in chromatin remodeling during DNA repair"*
**作者**: Thoma, N.H., Kwon, Y., & Sung, P.
**摘要**: 通过X射线晶体学解析了重组RAD54L蛋白的ATP酶结构域结构，表明其通过水解ATP改变染色质构象，为DNA修复蛋白提供结合位点。
3. **文献名称**: *"Deficiency in RAD54L leads to impaired homologous recombination and genome instability"*
**作者**: Tan, T.L., Essers, J., & Kanaar, R.
**摘要**: 利用小鼠模型和细胞实验证明，RAD54L缺失会导致同源重组效率显著降低，引发染色体断裂和肿瘤易感性升高，强调其在维持基因组稳定性中的重要性。
4. **文献名称**: *"Biochemical characterization of recombinant human RAD54L DNA translocase activity"*
**作者**: Heyer, W.D., et al.
**摘要**: 体外实验表明，重组RAD54L蛋白具有ATP依赖的DNA链移位能力，并通过促进RAD51-ssDNA复合体的形成提升同源重组效率。
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**注**：以上内容基于领域内典型研究方向的模拟整理，实际文献请通过PubMed/Google Scholar检索确认。
**Background of Human RAD54L Protein**
RAD54L, a member of the RAD52 epistasis group, is a conserved DNA repair protein critical for maintaining genomic stability. It belongs to the Swi2/Snf2 family of ATP-dependent chromatin remodelers, characterized by helicase-like domains and ATPase activity. Functionally, RAD54L plays a central role in homologous recombination repair (HRR), a high-fidelity mechanism to resolve DNA double-strand breaks (DSBs). It interacts directly with RAD51. stabilizing nucleoprotein filaments on single-stranded DNA, facilitating homology search, and promoting strand invasion during HRR. Additionally, RAD54L enhances Holliday junction migration and modulates chromatin structure to enable efficient DNA repair.
Mutations or dysregulation of RAD54L are linked to genomic instability, cancer predisposition, and resistance to therapies targeting DNA damage, such as poly(ADP-ribose) polymerase (PARP) inhibitors. Studies highlight its dual role in tumor suppression (via error-free repair) and potential oncogenesis (through replication stress mitigation in cancer cells). Its expression patterns and functional interactions make RAD54L a biomarker candidate for predicting therapeutic responses in cancers reliant on HRR. Current research explores RAD54L as a target to sensitize tumors to radiation or chemotherapy, leveraging its pivotal role in DNA repair pathways.
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