| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAD51L3 |
| Uniprot No | O75771 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-328 aa |
| 活性数据 | MGVLRVGLCPGLTEEMIQLLRSHRIKTVVDLVSADLEEVAQKCGLSYKALVALRRVLLAQFSAFPVNGADLYEELKTSTAILSTGIGSLDKLLDAGLYTGEVTEIVGGPGSGKTQVCLCMAANVAHGLQQNVLYVDSNGGLTASRLLQLLQAKTQDEEEQAEALRRIQVVHAFDIFQMLDVLQELRGTVAQQVTGSSGTVKVVVVDSVTAVVSPLLGGQQREGLALMMQLARELKTLARDLGMAVVVTNHITRDRDSGRLKPALGRSWSFVPSTRILLDTIEGAGASGGRRMACLAKSSRQPTGFQEMVDIGTWGTSEQSATLQGDQT |
| 分子量 | 62 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAD51同源蛋白家族(可能与RAD51L3相关)的3篇代表性文献，涵盖其功能及疾病关联的研究：
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1. **文献名称**: **"RAD51 paralogs: roles in DNA damage signalling, recombinational repair and cancer"**
**作者**: Prakash R. et al.
**摘要**: 系统综述RAD51家族旁系同源蛋白(RAD51B/C/D/XRCC2/XRCC3)在DNA双链断裂修复中的协作机制，强调其在同源重组(HR)和基因组稳定性中的作用，并讨论其突变与乳腺癌、卵巢癌易感性的关联。
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2. **文献名称**: **"Structural insights into RAD51C-mediated tumor suppression"**
**作者**: Rodrigue A. et al.
**摘要**: 通过X射线晶体学解析RAD51C(可能对应RAD51L2/L3)的结构，提出其与XRCC3结合形成的复合物在稳定DNA修复中间体的关键作用，并揭示其功能缺陷导致范可尼贫血表型的分子机制。
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3. **文献名称**: **"RAD51D-deficient cells exhibit defects in homologous recombination and genome maintenance"**
**作者**: Chun J. et al.
**摘要**: 研究发现RAD51D(部分文献称RAD51L3)敲除的细胞中，同源重组效率显著下降，导致染色体断裂累积和细胞凋亡增加，证实其作为肿瘤抑制因子在卵巢癌中的潜在治疗靶点价值。
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**备注**：
- 因蛋白质命名可能存在差异(如RAD51L3或为RAD51D/C的别名)，建议结合基因编号(如RAD51C: HGNC基因ID 9820)或上下文进一步验证。
- 更多最新研究可检索PubMed关键词：**RAD51 paralogs + DNA repair**。
**Background of Human Recombinant RAD51L3 Protein**
The human RAD51L3 protein, also known as RAD51C, is a crucial member of the RAD51 paralog family involved in DNA homologous recombination repair (HRR), a vital mechanism for maintaining genomic stability. It shares homology with the RAD51 recombinase but functions as an accessory factor, facilitating critical steps in HRR, such as strand pairing and Holliday junction resolution. RAD51L3 forms complexes with other paralogs (e.g., XRCC3. RAD51B) to stabilize DNA repair intermediates and mediate interactions with BRCA1/BRCA2. linking it to Fanconi anemia and hereditary cancer pathways.
Structurally, RAD51L3 contains conserved ATP-binding Walker motifs and interacts with DNA through its N-terminal domain. Its recombinant form is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study molecular mechanisms of HRR, screen DNA repair-targeting drugs, or model pathogenic mutations linked to ovarian, breast, and other cancers. Dysregulation or mutations in RAD51L3 impair HRR efficiency, leading to chromosomal aberrations and tumorigenesis. As a therapeutic target, recombinant RAD51L3 aids in deciphering synthetic lethality strategies for cancers with defective DNA repair, such as BRCA-deficient tumors. Research continues to explore its role in meiosis, replication stress response, and potential applications in precision oncology.
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