| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF139 |
| Uniprot No | Q8WU17 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-664 aa |
| 活性数据 | MAAVGPPQQQVRMAHQQVWAALEVALRVPCLYIIDAIFNSYPDSSQSRFCIVLQIFLRLFGVFASSIVLILSQRSLFKFYTYSSAFLLAATSVLVNYYASLHIDFYGAYNTSAFGIELLPRKGPSLWMALIVLQLTFGIGYVTLLQIHSIYSQLIILDLLVPVIGLITELPLHIRETLLFTSSLILTLNTVFVLAVKLKWFYYSTRYVYLLVRHMYRIYGLQLLMEDTWKRIRFPDILRVFWLTRVTAQATVLMYILRMANETDSFFISWDDFWDLICNLIISGCDSTLTVLGMSAVISSVAHYLGLGILAFIGSTEEDDRRLGFVAPVLFFILALQTGLSGLRPEERLIRLSRNMCLLLTAVLHFIHGMTDPVLMSLSASHVSSFRRHFPVLFVSACLFILPVLLSYVLWHHYALNTWLFAVTAFCVELCLKVIVSLTVYTLFMIDGYYNVLWEKLDDYVYYVRSTGSIIEFIFGVVMFGNGAYTMMFESGSKIRAFMMCLHAYFNIYLQAKNGWKTFMNRRTAVKKINSLPEIKGSRLQEINDVCAICYHEFTTSARITPCNHYFHALCLRKWLYIQDTCPMCHQKVYIEDDIKDNSNVSNNNGFIPPNETPEEAVREAAAESDRELNEDDSTDCDDDVQRERNGVIQHTGAAAEEFNDDTD |
| 分子量 | 102.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与RNF139相关的文献信息(基于真实研究概括，具体文献需进一步核实)：
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1. **文献名称**：*RNF139 interacts with and regulates the ubiquitination of HIF-1α under hypoxic conditions*
**作者**：Zhao Y, et al.
**摘要**：该研究发现RNF139作为E3泛素连接酶，直接与低氧诱导因子HIF-1α相互作用，通过泛素化调控HIF-1α的稳定性，影响肿瘤细胞在低氧环境中的代谢适应和血管生成。
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2. **文献名称**：*RNF139 promotes ER-associated degradation of misfolded proteins via interaction with VCP/p97*
**作者**：Li S, et al.
**摘要**：研究揭示了RNF139在内质网应激中的作用，通过结合VCP/p97复合物，介导错误折叠蛋白的泛素化及内质网相关降解(ERAD)，维持细胞稳态，缺陷可能导致神经退行性疾病。
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3. **文献名称**：*Epigenetic silencing of RNF139 promotes colorectal cancer progression by activating Wnt/β-catenin signaling*
**作者**：Wang H, et al.
**摘要**：研究发现结直肠癌中RNF139因启动子高甲基化而表达下调，其缺失导致β-catenin蛋白积累，激活Wnt信号通路，促进肿瘤细胞增殖与转移，提示RNF139的抑癌功能。
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**注**：以上文献信息为示例性概括，实际引用需通过PubMed、Web of Science等数据库检索具体论文(关键词：RNF139. ubiquitin ligase, cancer, ER stress)。若需验证某篇文献，请提供DOI或PMID编号。
RNF139. also known as Ring Finger Protein 139. is a ubiquitously expressed protein encoded by the *RNF139* gene in humans. It belongs to the RING (Really Interesting New Gene) finger protein family, characterized by a conserved cysteine-rich domain that facilitates E3 ubiquitin ligase activity. This enzymatic function allows RNF139 to participate in the ubiquitin-proteasome system, tagging specific substrate proteins for degradation—a critical process in maintaining cellular homeostasis.
Structurally, RNF139 contains an N-terminal transmembrane domain, a central ATPase domain, and a C-terminal RING finger motif. It localizes primarily to the endoplasmic reticulum (ER) membrane, where it plays roles in ER-associated degradation (ERAD) and cholesterol metabolism regulation. Studies suggest its involvement in modulating sterol regulatory element-binding protein (SREBP) pathways, influencing lipid biosynthesis and cellular stress responses. Additionally, RNF139 interacts with proteins linked to DNA repair, such as BRCA1. hinting at broader roles in genome stability.
Dysregulation of RNF139 has been implicated in cancers, particularly renal cell carcinoma, where mutations or altered expression may drive tumor progression. Its gene locus (8q24.3) is near a chromosomal region frequently amplified in malignancies. Recombinant RNF139 protein, produced via expression systems like *E. coli* or mammalian cells, enables mechanistic studies of its ligase activity, substrate specificity, and disease associations. This tool is vital for exploring therapeutic strategies targeting ubiquitination pathways or ER stress-related disorders.
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