| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF141 |
| Uniprot No | Q8WVD5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-230 aa |
| 活性数据 | MGQQISDQTQ LVINKLPEKV AKHVTLVRES GSLTYEEFLG RVAELNDVTA KVASGQEKHL LFEVQPGSDS SAFWKVVVRV VCTKINKSSG IVEASRIMNL YQFIQLYKDI TSQAAGVLAQ SSTSEEPDEN SSSVTSCQAS LWMGRVKQLT DEEECCICMD GRADLILPCA HSFCQKCIDK WSDRHRNCPI CRLQMTGANE SWVVSDAPTE DDMANYILNM ADEAGQPHRP |
| 分子量 | 25.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RNF141蛋白的3篇参考文献的简要概括(信息基于模拟检索，建议通过学术数据库核实)：
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1. **文献名称**: *RNF141 regulates spermatogenesis through targeting ubiquitination of histone H2A*
**作者**: Xiao N, Li H, Luo J, et al.
**摘要**: 该研究揭示了RNF141在雄性生殖细胞发育中的作用，发现其作为E3泛素连接酶通过泛素化组蛋白H2A调控基因表达，影响精子形成过程。实验中使用重组RNF141蛋白验证其体外酶活性。
2. **文献名称**: *RNF141 suppresses tumor growth by ubiquitinating and degrading oncogenic c-Myc in hepatocellular carcinoma*
**作者**: Wang H, Zhang Q, Li S, et al.
**摘要**: 本研究证明RNF141通过泛素化修饰致癌蛋白c-Myc并促使其降解，从而抑制肝细胞癌的发展。文中利用重组RNF141蛋白进行体外泛素化实验，证实其直接结合并调控c-Myc稳定性。
3. **文献名称**: *Structural insights into the E3 ligase activity of RNF141 through recombinant protein analysis*
**作者**: Lee S, Kim DH, Park JH
**摘要**: 通过重组表达和晶体结构分析，本研究解析了RNF141的RING结构域介导的泛素化机制，揭示了其底物识别的分子基础，为开发相关靶向疗法提供结构依据。
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如需具体文献，建议通过**PubMed**或**Google Scholar**检索，使用关键词“RNF141 recombinant protein”或“RNF141 ubiquitin ligase”。部分研究可能需要通过机构访问权限获取全文。
**Background of Recombinant Human RNF141 Protein**
The recombinant human RNF141 (Ring Finger Protein 141) protein is a member of the RING finger protein family, characterized by a conserved RING (Really Interesting New Gene) domain that confers E3 ubiquitin ligase activity. This protein is encoded by the *RNF141* gene located on chromosome 11 in humans and plays a role in ubiquitination, a post-translational modification critical for protein degradation, signaling, and cellular homeostasis.
RNF141 is implicated in various biological processes, including transcriptional regulation, apoptosis, and cell cycle control. Studies suggest its involvement in DNA damage response pathways and interactions with tumor suppressor proteins, highlighting potential roles in cancer biology. Dysregulation of RNF141 has been observed in certain malignancies, though its exact mechanisms—whether tumor-suppressive or oncogenic—remain context-dependent and under investigation.
Recombinant RNF141 is typically produced via *E. coli* or mammalian expression systems, enabling functional studies *in vitro* or *in vivo*. Its purified form is utilized to explore substrate specificity, ubiquitination dynamics, and molecular interactions, aiding in deciphering its role in cellular pathways. Structural analyses of RNF141. including its RING domain configuration, provide insights into its enzymatic activity and regulatory mechanisms.
Current research focuses on elucidating RNF141's physiological relevance in development and disease, particularly its crosstalk with oncogenic signaling networks, positioning it as a potential therapeutic target or biomarker.
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