| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAMD7 |
| Uniprot No | Q7Z3H4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-446 aa |
| 活性数据 | MAVNPLLTPT GQQTIPLIPS PFGPPTVDRD VLPSTVAPTD PRQFCVPSQF GSSVLPNTNM ANVLSSRIYP GWGILPPESI KAVARRNEMI QRHHTARTEM EMYAIYQQRR MEKINPKGLA GLGIPFLYGS SVPAAPAAYH GRSMLPAGDL HFHRSTLRNL QGNPMLAATA PHFEESWGQR CRRLRKNTGN QKALDSDAES SKSQAEEKIL GQTHAVPYEE DHYAKDPDIE APSNQKSSET NEKPTTALAN TCGELEPTHR KPWGSHTTTL KAKAWDDGKE EASEQIFATC DEKNGVCPPV PRPSLPGTHA LVTIGGNLSL DEDIQKWTVD DVHSFIRSLP GCSDYAQVFK DHAIDGETLP LLTEEHLRGT MGLKLGPALK IQSQVSQHVG SMFYKKTLSF PIRQAFDQPA DTSPLLDPNS WSDTMNIFCP QDTIIPKGIE RGSMRN |
| 分子量 | 49.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SAMD7蛋白的模拟参考文献示例(注：以下内容为虚构，实际文献需通过学术数据库查询)：
1. **《Structural and Functional Analysis of SAMD7 in Ocular Angiogenesis》**
*作者：Li X, Wang Y, et al.*
**摘要**：本研究解析了重组人SAMD7蛋白的晶体结构，揭示其通过抑制VEGF信号通路调控视网膜血管生成的分子机制，为治疗糖尿病视网膜病变提供新靶点。
2. **《Recombinant SAMD7 Expression and Its Role in Inflammatory Response》**
*作者：Chen J, Zhang H, et al.*
**摘要**：通过大肠杆菌系统成功表达重组人SAMD7蛋白，实验发现其能显著抑制巨噬细胞中NF-κB通路的激活，表明其在炎症性疾病治疗中的潜在应用价值。
3. **《SAMD7-Mediated Regulation of TGF-β Signaling in Fibrosis》**
*作者：Smith A, Patel R, et al.*
**摘要**：利用CHO细胞表达的重组SAMD7蛋白，证实其通过结合Smad4阻断TGF-β信号传导，显著减轻小鼠肝纤维化表型，为抗纤维化药物研发提供新策略。
4. **《Development of a High-Yield SAMD7 Recombinant Protein Production System》**
*作者：Kim S, Lee M, et al.*
**摘要**：优化哺乳动物细胞培养条件实现重组SAMD7的高效表达与纯化，并验证其稳定性及生物活性，为后续大规模生产和功能研究奠定基础。
**注意**：以上内容为示例，实际研究中需检索PubMed、Web of Science等平台获取真实文献。
**Recombinant Human SAMD7 Protein: Background**
The sterile alpha motif (SAM) domain-containing protein 7 (SAMD7) is a member of the SAMD family, characterized by conserved SAM domains that mediate protein-protein and protein-nucleic acid interactions. SAMD7 is implicated in regulating innate immune responses and cellular signaling pathways, potentially influencing inflammatory and stress-related processes. Structurally, SAMD7 contains a central SAM domain, which facilitates molecular recognition and complex formation, and disordered regions that may contribute to its functional versatility.
Recombinant human SAMD7 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*)*, enabling high-purity yields for research. Its production involves cloning the SAMD7 gene into expression vectors, followed by affinity chromatography purification (e.g., His-tag). This recombinant tool is critical for *in vitro* studies, including binding assays, structural analysis (e.g., crystallography), and functional investigations into its role in pathways like NF-κB or interferon signaling.
Emerging studies suggest SAMD7 may regulate transcriptional responses to pathogens or cellular damage, positioning it as a potential therapeutic target in autoimmune diseases, cancer, or viral infections. Its involvement in retinal function and angiogenesis has also been explored, highlighting broader biomedical relevance. Current research focuses on elucidating SAMD7’s interactome and mechanistic contributions to disease, underscoring its value in both basic and translational biology.
(Word count: 199)
×
