| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEC31A |
| Uniprot No | O94979 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-509 aa |
| 活性数据 | MKLKEVDRTAMQAWSPAQNHPIYLATGTSAQQLDATFSTNASLEIFELDLSDPSLDMKSCATFSSSHRYHKLIWGPYKMDSKGDVSGVLIAGGENGNIILYDPSKIIAGDKEVVIAQNDKHTGPVRALDVNIFQTNLVASGANESEIYIWDLNNFATPMTPGAKTQPPEDISCIAWNRQVQHILASASPSGRATVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDVATQMVLASEDDRLPVIQMWDLRFASSPLRVLENHARGILAIAWSMADPELLLSCGKDAKILCSNPNTGEVLYELPTNTQWCFDIQWCPRNPAVLSAASFDGRISVYSIMGGSTDGLRQKQVDKLSSSFGNLDPFGTGQPLPPLQIPQQTAQHSIVLPLKKPPKWIRRPVGASFSFGGKLVTFENVRMPSHQGAEQQQQQHHVFISQVVTEKEFLSRSDQLQQAVQSQGFINYCQKKIDASQTEFEKNVWSFLKVNFEDDSRGKYLELLGYRKEDLGKKVNFWES |
| 分子量 | 83.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SEC31A蛋白的3篇参考文献示例(注：以下为模拟文献，实际引用需以真实论文为准)：
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1. **文献名称**: *"Structural insights into the COPII coat complex component SEC31A"*
**作者**: Lee, C., et al.
**摘要**: 通过冷冻电镜技术解析了重组人SEC31A蛋白的结构，揭示了其WD40结构域与COPII复合体中其他组分(如SEC13)的结合模式，阐明了其在囊泡运输中的支架作用。
2. **文献名称**: *"SEC31A mutations disrupt endoplasmic reticulum export and cause cranio-lenticulo-sutural dysplasia"*
**作者**: Boyadjiev, S.A., et al.
**摘要**: 研究发现SEC31A基因突变会导致重组蛋白功能异常，破坏内质网至高尔基体的蛋白质转运，进而引发颅缝早闭等遗传性疾病，表明SEC31A在发育中的关键作用。
3. **文献名称**: *"Recombinant SEC31A as a biomarker for COPII vesicle formation in cancer cells"*
**作者**: Tang, Y., & Wang, Q.
**摘要**: 开发了重组SEC31A蛋白的高效表达系统，验证其作为COPII囊泡形成标志物的潜力，并发现其在肝癌细胞中异常高表达，提示与肿瘤分泌途径活跃性相关。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“recombinant SEC31A protein”、“SEC31A COPII”等。
SEC31A is a key component of the COPII (coat protein complex II) coat involved in endoplasmic reticulum (ER)-to-Golgi vesicular transport. As a scaffolding protein, it interacts with SEC24 and SEC23 to form the outer layer of the COPII coat, facilitating the budding of transport vesicles from the ER membrane. Structurally, SEC31A contains seven WD40 repeat domains that mediate protein-protein interactions, enabling assembly of the COPII lattice. It also possesses proline-rich regions and a C-terminal α-solenoid domain critical for membrane curvature induction. The human SEC31A gene encodes a ~130 kDa protein, often studied as a recombinant form expressed in mammalian or insect cell systems to preserve post-translational modifications like phosphorylation, which regulates COPII dynamics. Recombinant SEC31A is widely used in structural studies (e.g., cryo-EM analyses of COPII cage architecture) and functional assays exploring ER export, cargo sorting, and autophagy processes. Dysregulation of SEC31A is associated with developmental defects and diseases, including COPII-related disorders like cranio-lenticulo-sutural dysplasia. Its recombinant versions enable drug discovery targeting secretory pathway malfunctions in cancer and genetic disorders.
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