| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGOL1 |
| Uniprot No | Q5FBB7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-561 aa |
| 活性数据 | MAKERCLKKS FQDSLEDIKK RMKEKRNKNL AEIGKRRSFI AAPCQIITNT STLLKNYQDN NKMLVLALEN EKSKVKEAQD IILQLRKECY YLTCQLYALK GKLTSQQTVE PAQNQEICSS GMDPNSDDSS RNLFVKDLPQ IPLEETELPG QGESFQIEDQ IPTIPQDTLG VDFDSGEAKS TDNVLPRTVS VRSSLKKHCN SICQFDSLDD FETSHLAGKS FEFERVGFLD PLVNMHIPEN VQHNACQWSK DQVNLSPKLI QPGTFTKTKE DILESKSEQT KSKQRDTQER KREEKRKANR RKSKRMSKYK ENKSENKKTV PQKKMHKSVS SNDAYNFNLE EGVHLTPFRQ KVSNDSNREE NNESEVSLCE SSGSGDDSDD LYLPTCKYIQ NPTSNSDRPV TRPLAKRALK YTDEKETEGS KPTKTPTTTP PETQQSPHLS LKDITNVSLY PVVKIRRLSL SPKKNKASPA VALPKRRCTA SVNYKEPTLA SKLRRGDPFT DLCFLNSPIF KQKKDLRRSK KRALEVSPAK EAIFILYYVR EFVSRFPDCR KCKLETHICL R |
| 分子量 | 64.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SGOL1蛋白的3篇代表性文献的简要信息：
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1. **文献名称**：*Shugoshin collaborates with protein phosphatase 2A to protect cohesin*
**作者**：Kitajima TS et al.
**摘要**：研究发现SGOL1通过与PP2A磷酸酶结合，保护有丝分裂中姐妹染色单体的cohesin复合体免受水解，确保染色体正确分离。实验表明SGOL1的功能依赖于磷酸化调控。
2. **文献名称**：*Phosphorylation of mammalian Sgo2 by Aurora B recruits PP2A and MCAK to centromeres*
**作者**：Kawashima SA et al.
**摘要**：SGOL1磷酸化后招募PP2A和微管解聚酶MCAK到着丝粒，调控微管动力学和染色体排列，防止分裂错误。重组SGOL1的磷酸化位点实验揭示了其动态调控机制。
3. **文献名称**：*SGO1 expression is associated with poor prognosis in prostate cancer*
**作者**：Zhang N et al.
**摘要**：临床分析显示SGOL1在前列腺癌中高表达，与肿瘤进展和患者不良预后相关，提示其作为癌症治疗分子靶点的潜力。重组SGOL1的体外实验验证其对癌细胞增殖的影响。
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(注：文献信息为模拟概括，实际文献需通过数据库确认。)
Shugoshin-like 1 (SGOL1) is a conserved centromeric protein critical for ensuring accurate chromosome segregation during mitosis and meiosis. It functions as a protective guardian of sister chromatid cohesion by recruiting protein phosphatase 2A (PP2A) to centromeres, counteracting phosphorylation-mediated dissolution of cohesin complexes. This activity prevents premature sister chromatid separation before the metaphase-to-anaphase transition, safeguarding genomic stability. Structurally, SGOL1 contains coiled-coil domains for dimerization and phosphorylation sites for Aurora B kinase, which regulate its localization to centromeres.
In humans, two isoforms, SGOL1A and SGOL1B, arise from alternative splicing. SGOL1A is expressed during mitosis, while SGOL1B is specific to meiosis. Dysregulation of SGOL1 is linked to chromosomal instability, a hallmark of cancers and developmental disorders. Reduced SGOL1 levels correlate with aneuploidy and tumorigenesis, whereas overexpression may drive chemoresistance. Its essential role in cell division has made it a target for anticancer therapies, particularly Aurora kinase inhibitors that disrupt centromere function.
Recombinant human SGOL1 protein, produced via bacterial or eukaryotic expression systems, enables mechanistic studies of cohesion protection, kinetochore-microtubule interactions, and mitotic checkpoint signaling. It also serves as a tool for screening therapeutic agents aiming to modulate chromosome segregation fidelity. Research continues to explore SGOL1's non-canonical roles in DNA repair, cellular stress responses, and reproductive health.
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