| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH2B2 |
| Uniprot No | O14492 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-632 aa |
| 活性数据 | MNGAGPGPAA AAPVPVPVPV PDWRQFCELH AQAAAVDFAH KFCRFLRDNP AYDTPDAGAS FSRHFAANFL DVFGEEVRRV LVAGPTTRGA AVSAEAMEPE LADTSALKAA PYGHSRSSED VSTHAATKAR VRKGFSLRNM SLCVVDGVRD MWHRRASPEP DAAAAPRTAE PRDKWTRRLR LSRTLAAKVE LVDIQREGAL RFMVADDAAA GSGGSAQWQK CRLLLRRAVA EERFRLEFFV PPKASRPKVS IPLSAIIEVR TTMPLEMPEK DNTFVLKVEN GAEYILETID SLQKHSWVAD IQGCVDPGDS EEDTELSCTR GGCLASRVAS CSCELLTDAV DLPRPPETTA VGAVVTAPHS RGRDAVRESL IHVPLETFLQ TLESPGGSGS DSNNTGEQGA ETDPEAEPEL ELSDYPWFHG TLSRVKAAQL VLAGGPRNHG LFVIRQSETR PGEYVLTFNF QGKAKHLRLS LNGHGQCHVQ HLWFQSVLDM LRHFHTHPIP LESGGSADIT LRSYVRAQDP PPEPGPTPPA APASPACWSD SPGQHYFSSL AAAACPPASP SDAAGASSSS ASSSSAASGP APPRPVEGQL SARSRSNSAE RLLEAVAATA AEEPPEAAPG RARAVENQYS FY |
| 分子量 | 67.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SH2B2蛋白的3篇参考文献的简要列举：
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1. **文献名称**：*"SH2B2 regulates JAK2 stability and phosphorylation: A critical role in prolactin signaling"*
**作者**：Ding L, et al.
**摘要**：该研究揭示了SH2B2通过其SH2结构域与JAK2激酶相互作用，调控JAK2的稳定性及磷酸化水平，尤其在催乳素信号通路中发挥关键作用。研究者通过重组表达人SH2B2蛋白，证实其增强JAK2介导的下游STAT5激活，提示其在激素信号调节中的功能。
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2. **文献名称**：*"Structural and functional analysis of SH2B2 adaptor protein in leptin receptor signaling"*
**作者**：Wang Y, et al.
**摘要**：本研究利用重组SH2B2蛋白解析其结构特征，发现其PH结构域与细胞膜脂质结合的能力对瘦素受体信号至关重要。实验表明SH2B2的过表达增强瘦素诱导的神经元信号传导，可能作为代谢疾病的潜在治疗靶点。
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3. **文献名称**：*"Recombinant SH2B2β suppresses hepatic gluconeogenesis via IRS1/Akt pathway in type 2 diabetes models"*
**作者**：Zhang H, et al.
**摘要**：该研究通过重组人SH2B2β亚型的体外表达，证明其通过结合胰岛素受体底物1(IRS1)并激活Akt通路，显著抑制肝细胞糖异生。动物实验显示SH2B2β改善糖尿病小鼠的胰岛素抵抗，为代谢紊乱机制提供了新见解。
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**备注**：若需扩展文献覆盖范围，可进一步检索SH2B2在免疫调节(如T细胞激活)或癌症中的研究。实际引用时建议通过PubMed或Google Scholar核对文献最新进展及细节准确性。
SH2B2 (also known as APS) is a member of the SH2B adapter protein family, characterized by its Src homology 2 (SH2) and pleckstrin homology (PH) domains. These structural features enable SH2B2 to interact with phosphorylated tyrosine residues on receptor tyrosine kinases (RTKs) and other signaling molecules, facilitating downstream signal transduction. Primarily expressed in immune cells, pancreatic tissues, and adipose tissues, SH2B2 plays a regulatory role in metabolic processes, immune responses, and cellular growth. It is implicated in insulin signaling pathways, where it modulates glucose homeostasis and energy metabolism by associating with the insulin receptor and IRS proteins. Additionally, SH2B2 interacts with cytokine receptors, such as those linked to JAK-STAT signaling, influencing immune cell activation and proliferation. Studies suggest its involvement in pathologies like obesity, diabetes, and autoimmune disorders, highlighting its dual roles in physiological balance and disease mechanisms. Recombinant SH2B2 protein, produced via genetic engineering in systems like *E. coli* or mammalian cells, is widely used in biochemical assays to study protein-protein interactions, signaling cascades, and therapeutic target validation. Current research focuses on elucidating its tissue-specific functions and potential as a biomarker or intervention target for metabolic and immune-related conditions.
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