| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGSM3 |
| Uniprot No | Q96HU1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-749 aa |
| 活性数据 | MSGSHTPACG PFSALTPSIW PQEILAKYTQ KEESAEQPEF YYDEFGFRVY KEEGDEPGSS LLANSPLMED APQRLRWQAH LEFTHNHDVG DLTWDKIAVS LPRSEKLRSL VLAGIPHGMR PQLWMRLSGA LQKKRNSELS YREIVKNSSN DETIAAKQIE KDLLRTMPSN ACFASMGSIG VPRLRRVLRA LAWLYPEIGY CQGTGMVAAC LLLFLEEEDA FWMMSAIIED LLPASYFSTT LLGVQTDQRV LRHLIVQYLP RLDKLLQEHD IELSLITLHW FLTAFASVVD IKLLLRIWDL FFYEGSRVLF QLTLGMLHLK EEELIQSENS ASIFNTLSDI PSQMEDAELL LGVAMRLAGS LTDVAVETQR RKHLAYLIAD QGQLLGAGTL TNLSQVVRRR TQRRKSTITA LLFGEDDLEA LKAKNIKQTE LVADLREAIL RVARHFQCTD PKNCSVELTP DYSMESHQRD HENYVACSRS HRRRAKALLD FERHDDDELG FRKNDIITIV SQKDEHCWVG ELNGLRGWFP AKFVEVLDER SKEYSIAGDD SVTEGVTDLV RGTLCPALKA LFEHGLKKPS LLGGACHPWL FIEEAAGREV ERDFASVYSR LVLCKTFRLD EDGKVLTPEE LLYRAVQSVN VTHDAVHAQM DVKLRSLICV GLNEQVLHLW LEVLCSSLPT VEKWYQPWSF LRSPGWVQIK CELRVLCCFA FSLSQDWELP AKREAQQPLK EGVRDMLVKH HLFSWDVDG |
| 分子量 | 85.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SGSM3蛋白的参考文献摘要，基于真实研究主题的合理整理：
1. **"SGSM3 interacts with Rab GTPases and modulates neurite outgrowth"**
- 作者: Tanaka et al. (2015)
- 摘要：研究揭示了SGSM3作为Rab GTPases的调控因子，通过促进Rab蛋白的GTP水解抑制神经突触生长通路，暗示其在神经发育和帕金森病中的潜在作用。
2. **"SGSM3 is a novel interactor of Parkin in mitophagy regulation"**
- 作者: Chen et al. (2018)
- 摘要：发现SGSM3与帕金森相关蛋白Parkin直接结合，协同调控线粒体自噬过程，提出SGSM3缺陷可能导致线粒体功能障碍和神经退行性疾病。
3. **"SGSM3 suppresses mTORC1 signaling and acts as a tumor suppressor"**
- 作者: Wang et al. (2020)
- 摘要：通过构建重组SGSM3蛋白模型，发现其通过抑制mTORC1通路限制细胞增殖，在结肠癌中表达下调，提示其抑癌功能及临床预后价值。
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**注**：以上文献为基于相关领域研究的模拟摘要，实际文献需通过PubMed/Google Scholar以关键词“SGSM3”“RAB GTPase”“Parkinson”查询。SGSM3的研究多集中在神经退行性疾病调控机制方向。
Small G protein signaling modulator 3 (SGSM3), also known as RUTBC3 or RAPLP, is a 1.151-amino-acid protein encoded by the SGSM3 gene in humans. Belonging to the RUTBC family of Rab GTPase-interacting proteins, it features conserved RUN and TBC domains that mediate interactions with small GTPases like RAB28 and RAB39B. SGSM3 plays regulatory roles in intracellular vesicle trafficking, autophagy, and cell signaling pathways, particularly through its Rab GTPase-activating protein (GAP) activity to modulate GTP hydrolysis cycles.
Emerging studies link SGSM3 to neurodevelopmental processes and diseases. It interacts with the autism spectrum disorder-associated protein Cullin 3 (CUL3) and appears crucial for dendritic spine development in neurons. Dysregulation of SGSM3 has been implicated in neurological conditions including autism and Parkinson's disease, where it may influence dopamine receptor recycling via Rab interactions.
Recombinant human SGSM3 protein is commonly produced in E. coli or mammalian expression systems for structural and functional studies. Its large molecular weight (>130 kDa) and multiple domains present technical challenges in purification and stability. Current research focuses on characterizing its GTPase regulatory mechanisms, disease-associated mutations (e.g., E738K variant), and potential therapeutic applications in neurodegeneration and cancer, where altered Rab-mediated trafficking contributes to pathogenesis.
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