| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH2D3A |
| Uniprot No | Q9BRG2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-576 aa |
| 活性数据 | MQVPQDGEDL AGQPWYHGLL SRQKAEALLQ QNGDFLVRAS GSRGGNPVIS CRWRGSALHF EVFRVALRPR PGRPTALFQL EDEQFPSIPA LVHSYMTGRR PLSQATGAVV SRPVTWQGPL RRSFSEDTLM DGPARIEPLR ARKWSNSQPA DLAHMGRSRE DPAGMEASTM PISALPRTSS DPVLLKAPAP LGTVADSLRA SDGQLQAKAP TKPPRTPSFE LPDASERPPT YCELVPRVPS VQGTSPSQSC PEPEAPWWEA EEDEEEENRC FTRPQAEISF CPHDAPSCLL GPQNRPLEPQ VLHTLRGLFL EHHPGSTALH LLLVDCQATG LLGVTRDQRG NMGVSSGLEL LTLPHGHHLR LELLERHQTL ALAGALAVLG CSGPLEERAA ALRGLVELAL ALRPGAAGDL PGLAAVMGAL LMPQVSRLEH TWRQLRRSHT EAALAFEQEL KPLMRALDEG AGPCDPGEVA LPHVAPMVRL LEGEEVAGPL DESCERLLRT LHGARHMVRD APKFRKVAAQ RLRGFRPNPE LREALTTGFV RRLLWGSRGA GAPRAERFEK FQRVLGVLSQ RLEPDR |
| 分子量 | 63.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SH2D3A蛋白的假设性参考文献示例，供参考。请注意，这些内容为推测性概括，实际文献需通过学术数据库查询确认：
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1. **标题**：*SH2D3A interacts with CRK to promote integrin-mediated cell migration in cancer invasion*
**作者**：Tamura K, et al.
**摘要**：研究揭示了SH2D3A通过SH2结构域与适配蛋白CRK结合，激活整合素信号通路，增强肿瘤细胞的迁移和侵袭能力，提示其在癌症转移中的潜在作用。
2. **标题**：*Structural basis of phosphotyrosine recognition by the SH2 domain of SH2D3A*
**作者**：Smith J, et al.
**摘要**：通过X射线晶体学解析了SH2D3A的SH2结构域三维结构，发现其通过独特的结合口袋特异性识别pTyr-X-X-Pro基序，为设计靶向该结构域的分子提供了依据。
3. **标题**：*SH2D3A modulates T-cell activation by bridging SLP-76 and TCR signaling pathways*
**作者**：Wang L, et al.
**摘要**：研究发现SH2D3A在T细胞中与信号分子SLP-76相互作用，增强T细胞受体(TCR)下游信号传导，促进IL-2分泌及免疫应答，揭示了其在适应性免疫中的调控机制。
4. **标题**：*SH2D3A overexpression as a prognostic biomarker in metastatic breast cancer*
**作者**：Chen H, et al.
**摘要**：临床数据分析显示，SH2D3A在乳腺癌组织中的高表达与淋巴结转移和患者生存率降低显著相关，提示其作为预后标志物和治疗靶点的潜力。
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**建议**：通过PubMed、Google Scholar等平台，以关键词“SH2D3A protein”“SH2D3A function”或“SH2D3A cancer”检索最新文献，获取真实研究数据。
SH2D3A (also known as NESK or HSH2) is a multi-domain adapter protein containing an N-terminal SH2 domain and a C-terminal RhoGEF-like domain. It plays roles in cellular signaling, particularly in cytoskeletal reorganization, cell migration, and immune regulation. The SH2 domain enables interaction with phosphorylated tyrosine residues on receptor tyrosine kinases or other signaling molecules, while the RhoGEF domain may modulate small GTPases like Cdc42 and Rac1. influencing actin dynamics.
Expressed in immune cells, neurons, and various tissues, SH2D3A regulates T-cell activation, dendritic cell motility, and integrin-mediated adhesion. It acts as a scaffold, bridging membrane receptors (e.g., CD6) to downstream effectors, such as MAPK pathways. Dysregulation links SH2D3A to pathologies: overexpression in breast or colorectal cancers correlates with metastasis, though conflicting studies suggest context-dependent tumor-suppressive roles. In neurodevelopment, it promotes axonal growth through interactions with guidance receptors.
Its recombinant form is used to study signaling crosstalk, receptor-ligand interactions, and therapeutic targeting. However, functional complexity arises from splice variants and post-translational modifications. Further research is needed to clarify its dual roles in cancer and immune signaling networks.
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