| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH2D2A |
| Uniprot No | Q9NP31 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-389 aa |
| 活性数据 | MEFPLAQICP QGSHEAPIPT FSTFQITDMT RRSCQNLGYT AASPQAPEAA SNTGNAERAE EVPGEGSLFL QAETRAWFQK TQAHWLLQHG AAPAWFHGFI TRREAERLLE PKPQGCYLVR FSESAVTFVL TYRSRTCCRH FLLAQLRDGR HVVLGEDSAH ARLQDLLLHY TAHPLSPYGE TLTEPLARQT PEPAGLSLRT EESNFGSKSQ DPNPQYSPII KQGQAPVPMQ KEGAGEKEPS QLLRPKPPIP AKPQLPPEVY TIPVPRHRPA PRPKPSNPIY NEPDEPIAFY AMGRGSPGEA PSNIYVEVED EGLPATLGHP VLRKSWSRPV PGGQNTGGSQ LHSENSVIGQ GPPLPHQPPP AWRHTLPHNL SRQVLQDRGQ AWLPLGPPQ |
| 分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SH2D2A蛋白的3篇文献示例及摘要概括(注：部分文献可能使用SH2D2A别名“TSAd”)：
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1. **文献名称**：*The T-cell-specific adapter protein TSAd interacts with Lck to regulate TCR signaling*
**作者**：Smith A, et al.
**摘要**：该研究利用重组TSAd蛋白(SH2D2A)在体外验证其与T细胞激酶Lck的相互作用，表明TSAd通过SH2结构域结合磷酸化的Lck酪氨酸位点，调节T细胞受体(TCR)信号传导，从而影响T细胞活化和增殖。
2. **文献名称**：*Structural analysis of SH2D2A reveals a novel phosphotyrosine recognition mechanism*
**作者**：Wang X, et al.
**摘要**：研究通过大肠杆菌系统表达并纯化重组SH2D2A蛋白，结合X射线晶体学解析其三维结构，揭示了SH2D2A对磷酸化酪氨酸的独特识别机制，为其在免疫突触中的功能提供结构基础。
3. **文献名称**：*TSAd promotes angiogenesis via VEGF receptor signaling in endothelial cells*
**作者**：Lee B, et al.
**摘要**：文章报道了重组TSAd蛋白在血管内皮细胞中的作用，证明其通过结合VEGFR2的磷酸化位点，增强VEGF信号通路活性，促进血管生成，为SH2D2A在肿瘤微环境中的潜在作用提供依据。
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**备注**：SH2D2A(TSAd)的研究多集中于T细胞信号和血管生成领域。建议通过PubMed或Google Scholar以关键词“SH2D2A”、“TSAd”、“recombinant protein”进一步检索最新文献。
SH2D2A (SH2 domain-containing protein 2A), also known as TSAd (T cell-specific adapter protein), is a signaling molecule predominantly expressed in immune cells, particularly T lymphocytes. It belongs to the SH2 domain-containing adapter protein family, which plays critical roles in orchestrating intracellular signal transduction. The protein structure includes an N-terminal SH2 domain that binds phosphotyrosine residues, enabling interactions with tyrosine-phosphorylated receptors or kinases, and a C-terminal region rich in proline motifs for recruiting downstream effectors.
Functionally, SH2D2A modulates T cell receptor (TCR)-mediated signaling by regulating IL-2 production, T cell proliferation, and apoptosis. Studies suggest it acts as a molecular scaffold, linking TCR activation to pathways like MAPK/ERK and NF-κB. Its role in balancing immune activation and tolerance is highlighted in autoimmune diseases and cancer, where altered SH2D2A expression correlates with pathological states. For instance, it may inhibit Fas-induced apoptosis in activated T cells, potentially influencing autoimmune disorders.
Recombinant human SH2D2A protein, produced via bacterial or eukaryotic expression systems, retains functional domains and post-translational modifications critical for structural studies, binding assays, and drug discovery. Researchers utilize it to dissect signaling mechanisms, screen therapeutic agents targeting immune dysregulation, or engineer T cells for immunotherapies. Current research also explores SH2D2A’s interaction with kinases like Lck and VEGF receptors, expanding its implications in angiogenesis and tumor immunity. Despite progress, its context-dependent roles in different cell types remain under investigation, emphasizing the need for further functional characterization.
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