| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH2B |
| Uniprot No | Q9NRF2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-426 aa |
| 活性数据 | MVQREELLSFMGAEEAAPDPAGVGRGGGVAGPPSGGGGQPQWQKCRLLLRSEGEGGGGSRLEFFVPPKASRPRLSIPCSSITDVRTTTALEMPDRENTFVVKVEGPSEYIMETVDAQHVKAWVSDIQECLSPGPCPATSPRPMTLPLAPGTSFLTRENTDSLELSCLNHSESLPSQDLLLGPSESNDRLSQGAYGGLSDRPSASISPSSASIAASHFDSMELLPPELPPRIPIEEGPPAGTVHPLSAPYPPLDTPETATGSFLFQGEPEGGEGDQPLSGYPWFHGMLSRLKAAQLALTGGTGSHGVFLVRQSETRRGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQSIFDMLEHFRVHPIPLESGGSSDVVLVSYVPSSQRQQGREQAGSHAGVCEGDGCHPDASCTLMPFGASDCVTDHLP |
| 分子量 | 71.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SH2B蛋白的3篇代表性文献及其摘要：
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**1. 文献名称**：*SH2B1 is required for JAK2-mediated leptin signaling*
**作者**：Rui L et al.
**摘要**：该研究揭示了SH2B1作为衔接蛋白，通过其SH2结构域直接结合活化的JAK2激酶，增强瘦素受体信号传导，促进细胞增殖和代谢调节。实验证明SH2B1敲除小鼠表现出瘦素抵抗和肥胖表型。
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**2. 文献名称**：*Structural basis for SH2B1-mediated activation of JAK2 tyrosine kinase*
**作者**：Duan C et al.
**摘要**：本研究通过X射线晶体学解析了SH2B1的SH2结构域与JAK2激酶磷酸化酪氨酸的结合模式，阐明了其特异性识别机制，为SH2B1在生长激素和细胞因子信号通路中的作用提供结构基础。
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**3. 文献名称**：*SH2B1 regulates glucose homeostasis via β-cell proliferation and insulin secretion*
**作者**：Ren D et al.
**摘要**：文章发现SH2B1通过促进胰岛β细胞增殖和增强胰岛素分泌改善糖代谢。重组SH2B1蛋白在体外可激活PI3K-Akt通路，提示其作为糖尿病治疗的潜在靶点。
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**4. 文献名称**：*Recombinant SH2B1 enhances insulin signaling in vitro and ameliorates insulin resistance in vivo*
**作者**：Li M et al.
**摘要**：研究展示了重组人SH2B1蛋白在体外增强胰岛素受体磷酸化，并在高脂饮食诱导的胰岛素抵抗小鼠模型中显著改善血糖水平，表明其治疗代谢紊乱的应用潜力。
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以上研究涵盖了SH2B1在信号传导、结构机制及代谢疾病中的关键作用。如需具体文献来源或发表年份，可进一步补充检索。
The SH2B family of adapter proteins, particularly SH2B1. plays critical roles in regulating cellular signaling pathways. These proteins contain multiple functional domains, including an N-terminal dimerization domain, a central pleckstrin homology (PH) domain, and a C-terminal Src homology 2 (SH2) domain, enabling interactions with tyrosine kinase receptors and other signaling components. SH2B1 is best characterized for its involvement in metabolic regulation, where it enhances signaling through key receptors such as the leptin receptor, insulin receptor, and insulin-like growth factor-1 receptor. It directly binds to JAK2. IRS1/2. and Trk kinases, modulating downstream pathways like JAK-STAT, PI3K-AKT, and MAPK cascades. Genetic studies link SH2B1 mutations to obesity, type 2 diabetes, and metabolic syndrome in humans, while SH2B1 knockout mice exhibit severe insulin resistance and hyperphagia. SH2B2 and SH2B3 (LNK) show distinct regulatory roles in immune and hematopoietic systems. Recombinant human SH2B proteins, typically produced in bacterial or mammalian expression systems, serve as essential tools for studying signaling mechanisms, protein interactions, and therapeutic potential in metabolic disorders. Their structural integrity and post-translational modifications are carefully controlled to ensure functional relevance in biochemical assays, cell-based studies, and preclinical models. Current research focuses on exploiting SH2B1's scaffolding properties for developing interventions in obesity-related pathologies.
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