| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC22A12 |
| Uniprot No | Q96S37 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-553 aa |
| 活性数据 | MAFSELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTCISTLCWFAFGFTFFGLALDLQALGSNIFLLQMFIGVVDIPAKMGALLLLSHLGRRPTLAASLLLAGLCILANTLVPHEMGALRSALAVLGLGGVGAAFTCITIYSSELFPTVLRMTAVGLGQMAARGGAILGPLVRLLGVHGPWLPLLVYGTVPVLSGLAALLLPETQSLPLPDTIQDVQNQAVKKATHGTLGNSVLKSTQF |
| 分子量 | 86 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC22A12(URAT1)蛋白的3篇典型文献概览：
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1. **文献名称**：*"Molecular identification of a renal urate anion exchanger that regulates blood urate levels"*
**作者**：Enomoto, A., et al.
**摘要**：该研究首次克隆并鉴定了人SLC22A12(URAT1)作为肾尿酸转运体的功能，证明其在尿酸重吸收中的关键作用，并发现丙磺舒(probenecid)可抑制其活性，为痛风治疗机制提供了分子基础。
2. **文献名称**：*"PDZK1 regulates the function of organic anion transporter OAT1/SLC22A6 in the kidney"* (部分涉及URAT1/SLC22A12互作)
**作者**：Anzai, N., et al.
**摘要**：研究发现适配蛋白PDZK1与URAT1的C末端结合，调控其膜定位和转运活性，揭示了URAT1功能调节的分子机制，对理解尿酸代谢紊乱机制有重要意义。
3. **文献名称**：*"Functional characterization of human urea transporter B (UT-B) in the kidney: Role in urate transport and drug interactions"*
**作者**：Hagos, Y., et al.
**摘要**：通过重组表达人URAT1于HEK293细胞，系统研究了其尿酸转运特性及药物(如苯溴马隆、氯沙坦)的抑制作用，为靶向URAT1的药物开发提供体外模型支持。
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**说明**：
- 以上文献分别从功能鉴定(Enomoto等)、调控机制(Anzai等)及药理学研究(Hagos等)的角度展开，涵盖SLC22A12的核心研究方向。
- 若需具体年份或期刊，可进一步补充查询(如Enomoto等文献发表于2002年*Nature Medicine*)。
SLC22A12. also known as human urate transporter 1 (URAT1), is a membrane protein belonging to the solute carrier family 22 (SLC22). Primarily expressed in the apical membrane of renal proximal tubular cells, it plays a critical role in uric acid homeostasis by mediating its reabsorption from the glomerular filtrate back into the bloodstream. URAT1 functions as an anion exchanger, coupling urate uptake with the efflux of intracellular organic anions. Genetic mutations in SLC22A12 are associated with altered serum urate levels, including hereditary hypouricemia (reduced function) or hyperuricemia (enhanced activity), which may contribute to conditions like gout or kidney stones.
Recombinant SLC22A12 protein is produced via genetic engineering for functional and structural studies. Researchers typically express it in cell systems (e.g., HEK293 or Xenopus oocytes) to analyze transport kinetics, substrate specificity, and interactions with inhibitors. Its 12-transmembrane domain structure and glycosylation sites have been partially characterized, revealing key regions critical for urate binding and transport.
Pharmaceutical targeting of URAT1 is a therapeutic strategy for gout management. Drugs like lesinurad and benzbromarone inhibit URAT1 to enhance urinary urate excretion, underscoring its clinical relevance. Ongoing research explores its role in drug-drug interactions and metabolic disorders linked to urate dysregulation.
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