| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC2A8 |
| Uniprot No | Q9NY64 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-477 aa |
| 活性数据 | MTPEDPEETQPLLGPPGGSAPRGRRVFLAAFAAALGPLSFGFALGYSSPAIPSLQRAAPPAPRLDDAAASWFGAVVTLGAAAGGVLGGWLVDRAGRKLSLLLCSVPFVAGFAVITAAQDVWMLLGGRLLTGLACGVASLVAPVYISEIAYPAVRGLLGSCVQLMVVVGILLAYLAGWVLEWRWLAVLGCVPPSLMLLLMCFMPETPRFLLTQHRRQEAMAALRFLWGSEQGWEDPPIGAEQSFHLALLRQPGIYKPFIIGVSLMAFQQLSGVNAVMFYAETIFEEAKFKDSSLASVVVGVIQVLFTAVAALIMDRAGRRLLLVLSGVVMVFSTSAFGAYFKLTQGGPGNSSHVAISAPVSAQPVDASVGLAWLAVGSMCLFIAGFAVGWGPIPWLLMSEIFPLHVKGVATGICVLTNWLMAFLVTKEFSSLMEVLRPYGAFWLASAFCIFSVLFTLFCVPETKGKTLEQITAHFEGR |
| 分子量 | 77.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人SLC2A8(GLUT8)蛋白的3篇文献示例(部分信息基于领域共识)：
1. **"Functional characterization of human SLC2A8 (GLUT8) in mammalian cells"**
- **作者**: Doe J. et al.
- **摘要**: 研究在HEK293细胞中表达重组人SLC2A8蛋白，证实其葡萄糖转运活性受胰岛素调控，并参与细胞内囊泡运输机制。
2. **"Expression and purification of recombinant human GLUT8 for structural analysis"**
- **作者**: Smith A. et al.
- **摘要**: 通过大肠杆菌表达系统获得高纯度SLC2A8重组蛋白，优化纯化条件并利用冷冻电镜初步解析其三维结构，揭示底物结合关键位点。
3. **"Role of SLC2A8 in testicular glucose metabolism: Insights from knockout mice"**
- **作者**: Lee C. et al.
- **摘要**: 结合重组蛋白功能实验与基因敲除模型，发现SLC2A8在睾丸组织中特异性高表达，可能通过调控生精细胞能量稳态影响雄性生育功能。
**注**：以上文献为示例性质，实际引用时建议通过PubMed或Web of Science核实具体研究内容及作者信息。
Recombinant human SLC2A8 protein, also known as glucose transporter type 8 (GLUT8), is a member of the solute carrier family 2 (SLC2) responsible for facilitative glucose transport. Encoded by the *SLC2A8* gene, it shares structural features with other GLUT proteins, including 12 transmembrane domains. Unlike many cell surface GLUTs, GLUT8 is primarily localized to intracellular compartments such as endosomes and the endoplasmic reticulum, suggesting a role in intracellular glucose shuttling. It exhibits low glucose affinity and may transport other hexoses, though its precise substrates remain under investigation.
GLUT8 is highly expressed in metabolically active or glucose-sensitive tissues, including the brain, testis, adipose tissue, and liver. Physiological studies implicate it in energy homeostasis, neuronal survival, and spermatogenesis, potentially influencing insulin signaling and stress responses. Its intracellular sequestration implies regulation through trafficking mechanisms, where stimuli like hormones or cellular stress may trigger membrane translocation for glucose uptake.
Pathologically, GLUT8 dysregulation is linked to metabolic disorders, including obesity and type 2 diabetes, due to altered glucose metabolism. Emerging evidence also associates it with neurodegenerative conditions and cancer, where overexpression might support glycolytic tumor growth. Recombinant SLC2A8 proteins are utilized to study transport kinetics, structure-function relationships, and therapeutic targeting, offering insights into metabolic disease mechanisms and drug development. Ongoing research aims to clarify its physiological niche and therapeutic potential.
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