| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC2A4RG |
| Uniprot No | Q9NR83 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-387 aa |
| 活性数据 | MERPPPRG RDPSALRAEA PWLRAEGPGP RPVTVPTP PQGSSVGGGF AGLEFARPQE SEPRASDLGA PRTWTGAG PRTPSAHIPV PAQRATPGKA RLDEVM LTSLSTSPLL LGAPVFSP EPGLEPWKEA LVRPPGSYSS SSNSGDWGWD LASDQSSPST PSPPLPPE HFLFGEPTLR KRKSPAQVMF QCLWKSCGKV LSTASAMQRH IRLVHLGRQA EPEQSDGEED FYYTELDVGV DTLTDGLSSL TPVSPTASMP PAFPRLELPE LLEPPALPSP LRPPAPPLPP PPVLSTVANP QSCHSDRVYQ GCLTPARLEP QPTEVGACPP ALSSRIGVTL RKPRGDAKKC RKVYGMERRD LWCTACRWKK ACQRFLD |
| 分子量 | 41.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC2A4RG蛋白的模拟参考文献，内容基于该蛋白的已知生物学功能推测整理：
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1. **标题**：*SLC2A4RG modulates GLUT4 expression via transcriptional activation in insulin-sensitive tissues*
**作者**：Chen et al., 2018
**摘要**：研究揭示了SLC2A4RG作为转录因子直接结合SLC2A4(GLUT4)基因启动子，增强其表达。通过重组SLC2A4RG蛋白的体外过表达实验，发现其显著促进脂肪细胞和肌细胞的葡萄糖摄取，为胰岛素抵抗机制提供了新见解。
2. **标题**：*Crystal structure of human SLC2A4RG reveals a zinc-finger domain critical for DNA binding*
**作者**：Kumar et al., 2020
**摘要**：首次解析了重组人SLC2A4RG蛋白的晶体结构，发现其C端锌指结构域是DNA结合的关键区域。研究通过体外重组蛋白与DNA的互作实验，揭示了其调控靶基因的结构基础。
3. **标题**：*SLC2A4RG deficiency exacerbates high-fat diet-induced metabolic syndrome in mice*
**作者**：Wang et al., 2019
**摘要**：利用重组SLC2A4RG蛋白对基因敲除小鼠进行干预，发现其可通过恢复GLUT4表达改善胰岛素敏感性和糖代谢紊乱，表明其在代谢疾病中的潜在治疗价值。
4. **标题**：*Proteomic analysis of SLC2A4RG-interacting proteins in hepatocellular carcinoma*
**作者**：Garcia-Reyes et al., 2021
**摘要**：通过重组SLC2A4RG蛋白的亲和纯化筛选互作蛋白，鉴定出多个参与糖代谢和细胞增殖的因子，提示其在肝癌中通过调控代谢重编程影响肿瘤进展。
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**注**：以上文献为示例性内容，实际研究中可能存在差异，建议通过学术数据库(如PubMed)以关键词“SLC2A4RG”或“Hivep2”检索最新文献。
The SLC2A4RG (SLC2A4 Regulator) protein, also known as GLUT4 regulator, is a transcription factor involved in regulating the expression of glucose transporter 4 (GLUT4), a key mediator of insulin-stimulated glucose uptake in adipose and muscle tissues. This protein belongs to the Mondo family of transcription regulators and is critical for maintaining glucose homeostasis. It binds to the promoter region of the SLC2A4 gene, enhancing GLUT4 expression under insulin signaling. Dysregulation of SLC2A4RG has been implicated in metabolic disorders like type 2 diabetes, where impaired GLUT4 trafficking contributes to insulin resistance.
Recombinant human SLC2A4RG protein is engineered using expression systems (e.g., E. coli or mammalian cells) for in vitro studies. Its modular structure includes functional domains for DNA binding and protein-protein interactions. Researchers utilize this recombinant protein to investigate transcriptional mechanisms of glucose metabolism, screen potential therapeutic agents, and explore its role in cellular energy sensing. Recent studies also suggest its involvement in cancer progression, linking aberrant SLC2A4RG activity to tumor metabolism. However, detailed molecular pathways remain under investigation, highlighting its potential as a target for metabolic and oncological therapies.
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