| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC33A1 |
| Uniprot No | O00400 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-549 aa |
| 活性数据 | MSPTISHKDSSRQRRPGNFSHSLDMKSGPLPPGGWDDSHLDSAGREGDREALLGDTGTGDFLKAPQSFRAELSSILLLLFLYVLQGIPLGLAGSIPLILQSKNVSYTDQAFFSFVFWPFSLKLLWAPLVDAVYVKNFGRRKSWLVPTQYILGLFMIYLSTQVDRLLGNTDDRTPDVIALTVAFFLFEFLAATQDIAVDGWALTMLSRENVGYASTCNSVGQTAGYFLGNVLFLALESADFCNKYLRFQPQPRGIVTLSDFLFFWGTVFLITTTLVALLKKENEVSVVKEETQGITDTYKLLFAIIKMPAVLTFCLLILTAKIGFSAADAVTGLKLVEEGVPKEHLALLAVPMVPLQIILPLIISKYTAGPQPLNTFYKAMPYRLLLGLEYALLVWWTPKVEHQGGFPIYYYIVVLLSYALHQVTVYSMYVSIMAFNAKVSDPLIGGTYMTLLNTVSNLGGNWPSTVALWLVDPLTVKECVGASNQNCRTPDAVELCKKLGGSCVTALDGYYVESIICVFIGFGWWFFLGPKFKKLQDEGSSSWKCKRNN |
| 分子量 | 87.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC33A1蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*Mutations in SLC33A1 cause a lethal autosomal-recessive disorder with features of congenital cataracts, hearing loss, and neurodegeneration*
**作者**：Huppke, P. et al.
**摘要**：该研究首次报道了SLC33A1基因突变与新生儿致命性神经退行性疾病的关联，揭示了SLC33A1蛋白在乙酰辅酶A转运及细胞乙酰化过程中的核心作用，并通过模型验证其功能异常导致的病理表型。
2. **文献名称**：*SLC33A1-dependent lysosomal acetyl-CoA synthesis drives cancer cell immunoevasion and metastasis*
**作者**：Lin, Z. et al.
**摘要**：研究通过重组SLC33A1蛋白功能分析，发现其通过调控溶酶体乙酰-CoA水平影响肿瘤免疫逃逸和转移机制，为靶向SLC33A1的癌症治疗策略提供了理论基础。
3. **文献名称**：*Structural basis of acetyl-CoA transport by the mitochondrial carrier SLC33A1*
**作者**：Wang, Y. et al.
**摘要**：该研究通过冷冻电镜解析重组人SLC33A1蛋白的三维结构，阐明其跨膜转运乙酰-CoA的分子机制，揭示了关键氨基酸位点对底物识别及运输的调控作用。
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以上文献聚焦于SLC33A1的疾病关联、功能机制及结构解析，建议通过PubMed或Web of Science以标题或作者名检索获取全文。
**Background of Recombinant Human SLC33A1 Protein**
SLC33A1. also known as acetyl-CoA transporter 1 (AT-1), is a key transmembrane protein encoded by the *SLC33A1* gene located on chromosome 3q25.1. It primarily localizes to the endoplasmic reticulum (ER) membrane and functions as an acetyl-CoA transporter, facilitating the transfer of cytosolic acetyl-CoA into the ER lumen. This process is critical for post-translational acetylation of proteins, including those involved in glycosylation, such as the ER-resident acetyltransferases. SLC33A1 plays a vital role in maintaining cellular homeostasis, particularly in neurons, where it influences autophagy, oxidative stress responses, and synaptic function.
Mutations in *SLC33A1* are linked to severe neurodevelopmental disorders, such as Huppke-Brendel syndrome, characterized by congenital cataracts, hearing loss, and developmental delays. Dysregulation of SLC33A1 has also been implicated in neurodegenerative diseases, including Alzheimer's disease, possibly due to disrupted protein acetylation and ER stress.
Recombinant SLC33A1 protein, produced via mammalian expression systems, retains its native structural and functional properties, featuring 10 transmembrane domains and conserved residues essential for acetyl-CoA binding. It serves as a valuable tool for studying molecular mechanisms underlying SLC33A1-associated pathologies, drug screening for ER-related disorders, and elucidating its role in cellular metabolism. Research on recombinant SLC33A1 continues to advance insights into ER biology and therapeutic strategies for related diseases.
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