| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPRR2F |
| Uniprot No | Q96RM1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-72aa |
| 活性数据 | MSYQQQQCKQ PCQPPPVCPA PKCPEPCPPP KCPEPCPPSK CPQSCPPQQC QQKCPPVTPS PPCQPKCPPK SK |
| 分子量 | 7.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人SPRR2F蛋白的公开文献目前较为有限，且可能存在命名差异。以下是基于现有研究方向的假设性示例(建议通过学术数据库核实名称准确性后进一步检索)：
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1. **文献名称**: *"Expression and functional analysis of recombinant human SPRR2F in keratinocyte differentiation"*
**作者**: Smith A, et al.
**摘要**: 本研究报道了重组人SPRR2F蛋白在大肠杆菌中的高效表达及纯化方法。通过体外实验证实该蛋白在角质形成细胞分化过程中参与细胞膜稳定性调控，并与皮肤屏障功能相关。
2. **文献名称**: *"SPRR2F as a potential biomarker in epithelial barrier disorders"*
**作者**: Zhang L, et al.
**摘要**: 文章通过构建重组人SPRR2F蛋白，分析其在银屑病和特应性皮炎患者表皮中的表达异常，提出其可能作为上皮屏障功能障碍的分子标记物。
3. **文献名称**: *"Antimicrobial properties of SPRR2 family proteins: Focus on SPRR2F"*
**作者**: Müller R, et al.
**摘要**: 研究首次发现重组SPRR2F蛋白对金黄色葡萄球菌具有抑菌活性，提示其在先天免疫防御中的作用可能超出传统认知的结构蛋白功能。
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**注意事项**：
- **命名核实**：SPRR家族亚型(如2A/2B/2E等)较多，"2F"需确认是否为最新命名或笔误，建议检索时尝试变异拼写或扩展关键词(如"Small Proline-Rich Protein 2F")。
- **领域聚焦**：优先查阅皮肤病学、上皮生物学或口腔黏膜领域的期刊(如*Journal of Investigative Dermatology*)。
- **实验验证**：建议结合UniProt等数据库确认蛋白序列及已知功能，缩小检索范围。
如需具体文献，请提供更精确的命名信息或通过PubMed/Web of Science检索DOI。
The small proline-rich protein 2F (SPRR2F) is a member of the SPRR family, which comprises cysteine-rich proteins critical for epithelial barrier formation and cornified envelope assembly. Primarily expressed in stratified squamous epithelia, SPRR2F contributes to skin differentiation and stress response. Structurally, it contains conserved proline-rich domains and cross-linking motifs that enable interactions with loricrin, involucrin, and keratin intermediate filaments, reinforcing epidermal resilience against mechanical/chemical damage.
As a recombinant protein, SPRR2F is synthesized in vitro using expression systems (e.g., E. coli or mammalian cells) to study its biochemical properties without contamination from native tissue extracts. Research highlights its dual role: it strengthens epithelial integrity through disulfide bond-mediated cross-linking during terminal differentiation and modulates oxidative stress responses via antioxidant enzyme regulation. Dysregulation of SPRR2F is implicated in dermatopathies (e.g., psoriasis, eczema) and epithelial cancers, where abnormal cornified envelope dynamics affect tumor progression.
Recent studies also explore its potential in tissue engineering for skin repair and as a biomarker for epithelial malignancy. Despite functional overlaps with other SPRR isoforms, SPRR2F's unique expression patterns in specific epithelial subtypes make it a targeted focus for therapeutic interventions. Ongoing work aims to decode its post-translational modifications and signaling crosstalk in barrier-related pathologies.
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