| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRISP1 |
| Uniprot No | P54107 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-249aa |
| 氨基酸序列 | KKKSARDQF NKLVTDLPNV QEEIVNIHNA LRRRVVPPAS NMLKMSWSEE AAQNARIFSK YCDMTESNPL ERRLPNTFCG ENMHMTSYPV SWSSVIGVWY SESTSFKHGE WTTTDDDITT DHYTQIVWAT SYLIGCAIAS CRQQGSPRYL YVCHYCHEGN DPETKNEPYK TGVPCEACPS NCEDKLCTNP CIYYDEYFDC DIQVHYLGCN HSTTILFCKA TCLCDTEIK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于CRISP1重组蛋白研究领域的常见方向构建的示例性参考文献，供参考(注：文献为模拟示例，实际文献需通过学术数据库查询)：
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1. **标题**: *Recombinant CRISP1 modulates sperm calcium channels during capacitation*
**作者**: Silva MJ, et al.
**摘要**: 研究利用大肠杆菌表达系统制备重组CRISP1蛋白，发现其能特异性抑制CatSper钙离子通道活性，从而调控精子获能过程中的钙内流，提示其在受精中的关键作用。
2. **标题**: *Expression and functional characterization of CRISP1 in mammalian cells*
**作者**: Chen D, et al.
**摘要**: 通过哺乳动物细胞(HEK293)重组表达CRISP1.证实其与精子膜蛋白的相互作用，并证明其通过调节TRPM8离子通道影响精子运动能力。
3. **标题**: *CRISP1 as a potential target for immunocontraception: Insights from recombinant protein immunization*
**作者**: Gupta SK, et al.
**摘要**: 评估重组CRISP1作为免疫避孕抗原的潜力，动物实验显示其能诱导抗体反应，显著降低生育率，且无显著自身免疫副作用。
4. **标题**: *Structural insights into recombinant CRISP1 and its role in zona pellucida binding*
**作者**: Roberts KP, et al.
**摘要**: 解析重组CRISP1的晶体结构，揭示其C端结构域与透明带蛋白ZP2的结合机制，为理解受精的分子基础提供结构依据。
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**建议**：实际文献可通过PubMed或Google Scholar搜索关键词“recombinant CRISP1 protein”“CRISP1 expression”等获取。部分真实研究团队包括Roberto Buffone、Mariano G. Buffone(精子功能方向)及S.K. Gupta(免疫避孕方向)。
CRISP1 (Cysteine-Rich Secretory Protein 1) is a member of the CRISP family, which is characterized by conserved cysteine-rich domains and a secretory glycoprotein structure. Initially identified in mammalian reproductive tissues, CRISP1 is predominantly expressed in the epididymis and plays critical roles in sperm maturation, fertilization, and sperm-egg interaction. Its structure includes an N-terminal pathogenesis-related (PR) domain and a C-terminal cysteine-rich domain (CRD), which mediate interactions with ion channels and extracellular matrices.
Research has shown that CRISP1 binds to specific sites on sperm surfaces, modulating calcium channel activity to regulate sperm motility and acrosome reaction—a key step in fertilization. Dysregulation of CRISP1 is linked to male infertility, underscoring its functional importance. Recombinant CRISP1 protein, produced via heterologous expression systems (e.g., bacterial, insect, or mammalian cells), enables detailed mechanistic studies. Purification typically involves affinity chromatography and refolding steps to preserve disulfide bond-dependent tertiary structure.
Beyond basic research, recombinant CRISP1 has translational potential. It serves as an antigen in immunocontraceptive vaccines, leveraging its role in fertility to induce immune-mediated infertility. Additionally, it is explored in biomedical contexts for modulating ion channels in diseases like hypertension or cancer. Challenges in production include ensuring proper post-translational modifications and functional folding, often necessitating eukaryotic expression systems. Overall, CRISP1 recombinant protein remains a valuable tool for dissecting fertilization biology and developing therapeutic applications.
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