| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TFDP2 |
| Uniprot No | Q14188 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-446 aa |
| 活性数据 | TAKNVGLTS TNAEVRGFID QNLSPTKGNI SFVAFPVSNT NSPTKILPKT LGPINVNVGP QMIISTPQRL TSSGSVLIGS PYTPAPAMVT QTHIAEATGW VPGDRKRARK FIDSDFSESK RSKKGDKNGK GLRHFSMKVC EKVQRKGTTS YNEVADELVS EFTNSNNHLA ADSAYDQKNI RRRVYDALNV LMAMNIISKE KKEIKWIGLP TNSAQECQNL EIEKQRRIER IKQKRAQLQE LLLQQIAFKN LVQRNRQNEQ QNQGPPALNS TIQLPFIIIN TSRKTVIDCS ISSDKFEYLF NFDNTFEIHD DIEVLKRMGM SFGLESGKCS LEDLKLAKSL VPKALEGYIT DISTGPSWLN QGLLLNSTQS VSNLDLTTGA TLPQSSVNQG LCLDAEVALA TGQFLAPNSH QSSSAASHCS ESRGETPCSF NDEDEEDDEE DSSSPE |
| 分子量 | 49.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人TFDP2蛋白相关的参考文献示例(部分为模拟示例，实际文献请核实)：
1. **"Functional characterization of recombinant human TFDP2 in cell cycle regulation"**
- **作者**: Li X, Wang H, Zhang Y
- **摘要**: 本研究通过大肠杆菌系统表达并纯化了重组人TFDP2蛋白，发现其通过与E2F转录因子结合调控细胞周期相关基因的表达，揭示了其在G1/S期转换中的关键作用。
2. **"Structural insights into TFDP2-E2F4 complex formation using recombinant proteins"**
- **作者**: Kumar S, et al.
- **摘要**: 利用重组表达的TFDP2和E2F4蛋白进行共结晶实验，解析了二者的复合物结构，揭示了结合界面的关键氨基酸残基及调控DNA结合的分子机制。
3. **"Recombinant TFDP2 suppresses tumor growth by modulating apoptotic pathways in lung cancer"**
- **作者**: Chen L, et al.
- **摘要**: 通过哺乳动物细胞系表达重组TFDP2.证明其过表达通过激活p53依赖的凋亡通路抑制肺癌细胞增殖，提示其潜在的肿瘤治疗价值。
4. **"Development of a high-yield TFDP2 protein production system in insect cells"**
- **作者**: Tanaka M, et al.
- **摘要**: 建立了基于杆状病毒-昆虫细胞系统的重组TFDP2高效表达与纯化工艺，优化后的蛋白可用于大规模功能研究及药物筛选。
*注：上述文献标题及内容为示例性质，建议通过PubMed、Google Scholar等平台以“recombinant TFDP2”“human TFDP2 protein”等关键词检索真实文献。*
**Background of Recombinant Human TFDP2 Protein**
Transcription Factor Dimerization Partner 2 (TFDP2), also known as DP-2. is a member of the E2F/DP transcription factor family. It plays a critical role in regulating cell cycle progression, DNA replication, and apoptosis by forming heterodimers with E2F proteins. These complexes bind to target gene promoters, modulating their expression. Unlike its paralog TFDP1. TFDP2 exhibits distinct tissue-specific expression patterns, particularly in embryonic tissues and certain cancers, suggesting unique regulatory roles in development and oncogenesis.
Recombinant human TFDP2 protein is engineered using biotechnological platforms (e.g., *E. coli* or mammalian cell systems) to retain functional domains, including the dimerization and DNA-binding regions. This protein serves as a vital tool for studying TFDP2-E2F interactions, chromatin remodeling, and mechanisms underlying cell proliferation disorders. Its applications extend to cancer research, as dysregulated TFDP2 expression correlates with tumor aggressiveness and chemoresistance. Additionally, structural analyses of recombinant TFDP2 provide insights into post-translational modifications (e.g., phosphorylation) that fine-tune its activity. Understanding TFDP2's molecular functions holds promise for developing targeted therapies against malignancies and developmental abnormalities.
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