| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TFCP2L1 |
| Uniprot No | Q9NZI6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-479 aa |
| 活性数据 | MLFWHTQPEH YNQHNSGSYL RDVLALPIFK QEEPQLSPEN EARLPPLQYV LCAATSPAVK LHEETLTYLN QGQSYEIRLL ENRKLGDFQD LNTKYVKSII RVVFHDRRLQ YTEHQQLEGW RWSRPGDRIL DIDIPLSVGI LDPRASPTQL NAVEFLWDPA KRASAFIQVH CISTEFTPRK HGGEKGVPFR VQIDTFKQNE NGEYTEHLHS ASCQIKVFKP KGADRKQKTD REKMEKRTAQ EKEKYQPSYE TTILTECSPW PDVAYQVNSA PSPSYNGSPN SFGLGEGNAS PTHPVEALPV GSDHLLPSAS IQDAQQWLHR NRFSQFCRLF ASFSGADLLK MSRDDLVQIC GPADGIRLFN AIKGRNVRPK MTIYVCQELE QNRVPLQQKR DGSGDSNLSV YHAIFLEELT TLELIEKIAN LYSISPQHIH RVYRQGPTGI HVVVSNEMVQ NFQDESCFVL STIKAESNDG YHIILKCGL |
| 分子量 | 54.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TFCP2L1蛋白的3篇模拟参考文献(基于领域背景合理虚构，非真实文献)：
1. **文献名称**：*TFCP2L1 Drives Pluripotency through Transcriptional Repression of Differentiation Genes*
**作者**：Ying QL, Smith A, et al.
**摘要**：研究揭示了TFCP2L1通过直接抑制分化相关基因(如Wnt/β-catenin通路靶点)维持胚胎干细胞自我更新的分子机制，重组人TFCP2L1蛋白体外实验证实其结合DNA并招募抑制复合体的能力。
2. **文献名称**：*Structural Insights into TFCP2L1-DNA Interaction by Cryo-EM*
**作者**：Li J, Watanabe S, et al.
**摘要**：利用冷冻电镜解析了重组人TFCP2L1蛋白与靶DNA复合体的三维结构，阐明其独特的螺旋-环-螺旋结构域结合DNA的模式，为设计调控干细胞命运的分子工具提供依据。
3. **文献名称**：*Recombinant TFCP2L1 Production in Mammalian Cells Enhances Reprogramming Efficiency*
**作者**：Kim T, Rossi F, et al.
**摘要**：报道了在HEK293细胞中高效表达纯化重组人TFCP2L1蛋白的工艺，并证实其可替代Oct4/Sox2组合，显著提升体细胞重编程为诱导多能干细胞的效率。
(注：以上内容为示例性虚构，实际文献需通过PubMed/Google Scholar等平台以“TFCP2L1”或“CRTR-1”为关键词检索获取。)
**Background of Recombinant Human TFCP2L1 Protein**
TFCP2L1 (Transcription Factor CP2-Like 1) is a member of the CP2 transcription factor family, characterized by its evolutionarily conserved AP2 DNA-binding domain. It plays a critical role in maintaining pluripotency and self-renewal in embryonic stem cells (ESCs) by regulating key signaling pathways, including LIF/STAT3 and Wnt/β-catenin. Studies highlight its interaction with core pluripotency factors like Oct4. Nanog, and Sox2. suggesting its involvement in early embryonic development and cell fate determination.
Structurally, TFCP2L1 contains multiple functional domains, including a transcriptional activation domain and nuclear localization signals. Its recombinant form is typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. The protein is purified via affinity tags (e.g., His-tag) for experimental use. Recombinant TFCP2L1 serves as a vital tool for studying stem cell biology, reprogramming mechanisms, and cancer research, as aberrant expression has been linked to malignancies like leukemia and hepatocellular carcinoma.
Recent research explores its potential in regenerative medicine, particularly in enhancing induced pluripotent stem cell (iPSC) generation. However, its dual role in promoting pluripotency and tumorigenesis underscores the need for precise regulatory studies. Ongoing efforts aim to elucidate its downstream targets and therapeutic applications while addressing challenges in delivery and specificity.
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