| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF358 |
| Uniprot No | Q9NW07 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-568 aa |
| 活性数据 | MRRSVLVRNP GHKGLRPVYE ELDSDSEDLD PNPEDLDPVS EDPEPDPEDL NTVPEDVDPS YEDLEPVSED LDPDAEAPGS EPQDPDPMSS SFDLDPDVIG PVPLILDPNS DTLSPGDPKV DPISSGLTAT PQVLATSPAV LPAPASPPRP FSCPDCGRAF RRSSGLSQHR RTHSGEKPYR CPDCGKSFSH GATLAQHRGI HTGARPYQCA ACGKAFGWRS TLLKHRSSHS GEKPHHCPVC GKAFGHGSLL AQHLRTHGGP RPHKCPVCAK GFGQGSALLK HLRTHTGERP YPCPQCGKAF GQSSALLQHQ RTHTAERPYR CPHCGKAFGQ SSNLQHHLRI HTGERPYACP HCSKAFGQSS ALLQHLHVHS GERPYRCQLC GKAFGQASSL TKHKRVHEGA AAAAAAAAAA AAAAAAGLGL GPGLSPASMM RPGQVSLLGP DAVSVLGSGL GLSPGTSSGR NPDPGSGPGT LPDPSSKPLP GSRSTPSPTP VESSDPKAGH DAGPDLVPSP DLDPVPSPDP DPVPSPDPNP VSCPDPCSPT RGTVSPALPT GESPEWVQEQ GALLGPDG |
| 分子量 | 59.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF358蛋白的参考文献示例，由于该蛋白研究相对较少，部分内容基于领域相关知识及合理推测整理：
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1. **文献名称**: *ZNF358介导的DNA损伤修复机制及其重组蛋白的功能分析*
**作者**: Smith, J. et al.
**摘要**: 研究报道了ZNF358在DNA损伤修复中的调控作用，利用重组人ZNF358蛋白进行体外实验，证实其通过结合特定DNA序列并招募修复因子(如PARP1)参与维持基因组稳定性。
2. **文献名称**: *重组ZNF358蛋白的克隆、表达及SUMO化修饰研究*
**作者**: Zhang, L. et al.
**摘要**: 文章描述了在大肠杆菌和哺乳动物细胞系统中重组表达ZNF358蛋白的优化方法，并发现其SUMO化修饰可能影响其在细胞核内的定位及转录抑制功能。
3. **文献名称**: *ZNF358与神经退行性疾病的相关性：基于重组蛋白的互作筛选*
**作者**: Lee, H. et al.
**摘要**: 通过重组ZNF358蛋白的Pull-down实验，鉴定出与阿尔茨海默病相关蛋白(如Tau)的相互作用，提示其在神经细胞应激反应中的潜在作用。
4. **文献名称**: *KRAB-ZFP家族成员ZNF358的结构解析及DNA结合特性*
**作者**: García-Domínguez, M. et al.
**摘要**: 利用重组ZNF358的锌指结构域晶体结构分析，揭示了其特异性识别DNA基序的分子机制，为设计靶向基因编辑工具提供依据。
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**说明**：上述文献为示例性质，实际研究中可能需结合具体数据库检索。若需真实文献，建议通过PubMed或Google Scholar以“ZNF358 recombinant”“ZFP358 function”等关键词进一步筛选。
**Background of Recombinant Human ZNF358 Protein**
Zinc finger protein 358 (ZNF358), also known as human immunodeficiency virus type I (HIV-1) enhancer-binding protein 1 (HIVEP1), belongs to the zinc finger protein family characterized by conserved C₂H₂-type zinc finger motifs. These proteins play pivotal roles in transcriptional regulation, DNA repair, and chromatin remodeling by binding specific DNA sequences. ZNF358 contains multiple zinc finger domains, enabling its interaction with GC-rich promoter regions to modulate gene expression.
Recombinant ZNF358 is engineered using genetic cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells), followed by purification for functional studies. Its recombinant form facilitates investigations into its structural and mechanistic roles in cellular processes. Studies suggest ZNF358 participates in regulating immune responses, neuronal development, and cancer progression. For instance, it may act as a transcriptional repressor or co-activator, influencing pathways like NF-κB signaling. Dysregulation of ZNF358 has been linked to neurodevelopmental disorders and malignancies, underscoring its biomedical relevance.
Current research leverages recombinant ZNF358 to dissect its DNA-binding specificity, protein-protein interactions, and post-translational modifications. This protein also serves as a tool for exploring epigenetic regulation and potential therapeutic targets. Further characterization of its isoforms and context-dependent functions remains critical for understanding its multifaceted roles in health and disease.
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