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Recombinant  Human ZNF35 Protein

  • 中文名: 重组人(ZNF35)蛋白
  • 别    名: ZNF35Zinc finger Protein 35; Zinc finger Protein HF.10
货号: PAX2000-12842
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点ZNF35
Uniprot NoP13682
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-527 aa
活性数据MTAELREAMA LAPWGPVKVK KEEEEEENFP GQASSQQVHS ENIKVWAPVQ GLQTGLDGSE EEEKGQNISW DMAVVLKATQ EAPAASTLGS YSLPGTLAKS EILETHGTMN FLGAETKNLQ LLVPKTEICE EAEKPLIISE RIQKADPQGP ELGEACEKGN MLKRQRIKRE KKDFRQVIVN DCHLPESFKE EENQKCKKSG GKYSLNSGAV KNPKTQLGQK PFTCSVCGKG FSQSANLVVH QRIHTGEKPF ECHECGKAFI QSANLVVHQR IHTGQKPYVC SKCGKAFTQS SNLTVHQKIH SLEKTFKCNE CEKAFSYSSQ LARHQKVHIT EKCYECNECG KTFTRSSNLI VHQRIHTGEK PFACNDCGKA FTQSANLIVH QRSHTGEKPY ECKECGKAFS CFSHLIVHQR IHTAEKPYDC SECGKAFSQL SCLIVHQRIH SGDLPYVCNE CGKAFTCSSY LLIHQRIHNG EKPYTCNECG KAFRQRSSLT VHQRTHTGEK PYECEKCGAA FISNSHLMRH HRTHLVE
分子量59 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下是基于ZNF35蛋白相关研究方向的模拟参考文献示例(请注意,以下内容为虚构,真实文献需通过学术数据库检索):

1. **文献名称**: "Cloning and Expression of Human Zinc Finger Protein ZNF35 in E. coli"

**作者**: Smith J, et al.

**摘要**: 本研究成功克隆了人ZNF35基因,并在大肠杆菌中实现了重组表达。通过His标签纯化获得高纯度蛋白,并验证其锌指结构域的DNA结合活性。

2. **文献名称**: "Functional Analysis of ZNF35 as a Transcriptional Repressor in Cancer Cells"

**作者**: Zhang L, et al.

**摘要**: 发现ZNF35在多种癌症细胞中低表达,通过重组蛋白实验证实其结合特定DNA序列并抑制靶基因转录,提示其可能的抑癌作用。

3. **文献名称**: "Structural Characterization of the ZNF35 Zinc Finger Domain by NMR"

**作者**: Tanaka K, et al.

**摘要**: 利用核磁共振技术解析了重组ZNF35蛋白锌指结构域的三维结构,揭示了其与DNA相互作用的分子机制。

4. **文献名称**: "ZNF35 Interaction with Histone Deacetylases in Epigenetic Regulation"

**作者**: Chen R, et al.

**摘要**: 通过免疫共沉淀和体外Pull-down实验,证明重组ZNF35蛋白与HDAC复合物相互作用,参与染色质修饰和基因沉默。

**备注**:以上为基于ZNF蛋白家族常见研究方向的模拟示例。实际文献请通过PubMed、Google Scholar等平台,以“ZNF35 recombinant protein”“ZNF35 function”等关键词检索近年发表的论文。


背景信息

**Background of Recombinant Human ZNF35 Protein**

The recombinant human Zinc Finger Protein 35 (ZNF35), a member of the Krüppel-associated box (KRAB) zinc finger protein (ZFP) family, is encoded by the *ZNF35* gene. Zinc finger proteins are characterized by tandem Cys2-His2 (C2H2) zinc finger motifs, which facilitate sequence-specific DNA binding, enabling roles in transcriptional regulation, chromatin remodeling, and epigenetic modulation. ZNF35. located on chromosome 3p21-p22. is implicated in gene silencing through its KRAB domain, which recruits co-repressors like KAP1 to establish heterochromatin.

Recombinant ZNF35 is produced via genetic engineering, often expressed in *E. coli* or mammalian systems, ensuring high purity and activity for functional studies. Its structural domains, including the N-terminal KRAB repression module and C-terminal zinc finger array, are critical for studying interactions with DNA targets and regulatory partners. Dysregulation of ZNF35 has been linked to cancers, particularly in 3p chromosomal regions prone to deletions in lung and renal carcinomas, suggesting tumor-suppressive potential.

Current research focuses on elucidating ZNF35’s role in cellular processes like proliferation, differentiation, and apoptosis, as well as its interplay with viral pathogens. Recombinant ZNF35 serves as a tool for exploring gene networks, epigenetic mechanisms, and therapeutic strategies targeting zinc finger-mediated gene regulation.


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