| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MOGAT2 |
| Uniprot No | Q3SYC2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-334aa |
| 氨基酸序列 | MVEFAPLFMPWERRLQTLAVLQFVFSFLALAEICTVGFIALLFTRFWLLTVLYAAWWYLDRDKPRQGGRHIQAIRCWTIWKYMKDYFPISLVKTAELDPSRNYIAGFHPHGVLAVGAFANLCTESTGFSSIFPGIRPHLMMLTLWFRAPFFRDYIMSAGLVTSEKESAAHILNRKGGGNLLGIIVGGAQEALDARPGSFTLLLRNRKGFVRLALTHGAPLVPIFSFGENDLFDQIPNSSGSWLRYIQNRLQKIMGISLPLFHGRGVFQYSFGLIPYRRPITTVVGKPIEVQKTLHPSEEEVNQLHQRYIKELCNLFEAHKLKFNIPADQHLEFC |
| 预测分子量 | 38,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MOGAT2重组蛋白的3篇参考文献的模拟示例(基于学术研究常见方向，非真实文献)：
1. **标题**：*Recombinant Expression and Functional Characterization of Human MOGAT2 in HEK293 Cells*
**作者**：Chen et al.
**摘要**：本研究在HEK293细胞中成功表达了重组人源MOGAT2蛋白，并系统分析了其酶动力学特性。结果显示，MOGAT2对单酰基甘油和脂肪酸底物具有高度特异性，为后续药物靶点研究提供了基础。
2. **标题**：*Crystal Structure of MOGAT2 Reveals Insights into Acyltransferase Mechanism*
**作者**：Wang et al.
**摘要**：通过大肠杆菌表达系统获得高纯度MOGAT2重组蛋白，并解析其三维晶体结构。结构分析揭示了其催化活性中心的关键氨基酸残基，阐明了MOGAT2在甘油三酯合成中的分子机制。
3. **标题**：*Development of a High-Throughput Assay for MOGAT2 Activity Using Recombinant Protein*
**作者**：Smith et al.
**摘要**：利用昆虫细胞表达的重组MOGAT2蛋白，建立了基于荧光底物的高通量酶活检测方法。该方法被应用于筛选潜在抑制剂，为代谢性疾病治疗药物开发提供了技术平台。
4. **标题**：*MOGAT2 Deficiency Modulates Lipid Metabolism in Mouse Model via Recombinant Protein Rescue*
**作者**：Kimura et al.
**摘要**：在MOGAT2基因敲除小鼠中，通过注射重组MOGAT2蛋白验证其恢复甘油三酯合成的功能，证明MOGAT2在肠道脂质吸收中的关键作用，提示其作为治疗靶点的潜力。
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**注**：以上文献为示例性质，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。建议使用关键词“MOGAT2 recombinant”“MOGAT2 expression”等进行查询。
**Background of MOGAT2 Recombinant Protein**
Monoacylglycerol O-acyltransferase 2 (MOGAT2) is a key enzyme in lipid metabolism, primarily involved in the biosynthesis of triglycerides (TGs). It catalyzes the acylation of monoacylglycerol (MAG) to diacylglycerol (DAG), a critical step in the glycerol-3-phosphate pathway. MOGAT2 is predominantly expressed in tissues with high lipid turnover, such as the small intestine and adipose tissue, where it facilitates dietary fat absorption and energy storage. Unlike other isoforms (e.g., MOGAT1 and MOGAT3), MOGAT2 exhibits unique substrate specificity and plays a non-redundant role in lipid homeostasis, making it a potential therapeutic target for metabolic disorders like obesity, diabetes, and cardiovascular diseases.
Recombinant MOGAT2 protein is engineered using heterologous expression systems, such as *E. coli*, yeast, or mammalian cells, to produce soluble and enzymatically active forms for functional studies. Structural characterization reveals conserved motifs typical of the membrane-bound O-acyltransferase (MBOAT) family, including a catalytic histidine residue and hydrophobic domains critical for substrate binding. However, challenges in purifying full-length membrane-associated MOGAT2 have driven the development of truncated or tagged variants to enhance solubility and yield.
Research on MOGAT2 recombinant protein has advanced understanding of its kinetic properties, regulatory mechanisms, and interactions with lipid substrates or inhibitors. These studies are pivotal for drug discovery, particularly in designing small-molecule modulators to control TG synthesis. Additionally, MOGAT2’s role in intestinal lipid absorption highlights its relevance in addressing diet-induced metabolic syndromes. Despite progress, further exploration of its tissue-specific functions and post-translational modifications is needed to unlock its full therapeutic potential.
In summary, MOGAT2 recombinant protein serves as a vital tool for elucidating lipid metabolism pathways and developing interventions for metabolic diseases.
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