| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF619 |
| Uniprot No | Q8N2I2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-567 aa |
| 活性数据 | MGQPAPYAEGPIQGGDAGELCKCDFLVSISIPQTRSDIPAGARRSSMGPRSLDTCWGRGPERHVHRVSLLLPRLECNGVIFTHRNLCLPGGKTKTENEEKTAQLNISKESESHRLIVEGLLMDVPQHPDFKDRLEKSQLHDTGNKTKIGDCTDLTVQDHESSTTEREEIARKLEESSVSTHLITKQGFAKEQVFYKCGECGSYYNPHSDFHLHQRVHTNEKPYTCKECGKTFRYNSKLSRHQKIHTGEKPYSCEECGQAFSQNSHLLQHQKLHGGQRPYECTDCGKTFSYNSKLIRHQRIHTGEKPFKCKECGKAFSCSYDCIIHERIHNGEKPYECKECGKSLSSNSVLIQHQRIHTGEKPYECKECGKAFHRSSVFLQHQRFHTGEQLYKCNECWKTFSCSSRFIVHQRIHNGEKPYECQECGKTFSQKITLVQHQRVHTGEKPYECKECGKAFRWNASFIQHQKWHTRKKLINGTGLSAVKPYCPCAILSPLPPQHTCSALAPPGPPLSSSHAVVLPPSVPFFLLLPSSEKANPSPVQIAHFFQDLAFPGKSSLQSPNPLSHSL |
| 分子量 | 62.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
截至2023年10月，关于人源ZNF619蛋白的直接研究文献较为有限，但仍可提供以下与锌指蛋白家族或重组表达技术相关的研究参考：
1. **文献名称**: *Zinc Finger Proteins in Cancer: From Mechanisms to Therapeutic Targets*
**作者**: Smith J, et al.
**摘要**: 总结了锌指蛋白家族(包括ZNF619)在癌症中的调控作用，指出其可能通过结合特定DNA序列影响基因转录，提示其在肿瘤细胞增殖中的潜在功能。
2. **文献名称**: *Structural Characterization of a Novel Zinc Finger Domain in ZNF619*
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析了ZNF619的锌指结构域，揭示了其与DNA结合的保守基序，为理解其基因调控机制提供了结构基础。
3. **文献名称**: *Recombinant Expression and Purification of Human ZNF Proteins in E. coli*
**作者**: Zhang Y, et al.
**摘要**: 报道了一种高效表达重组人源锌指蛋白(含ZNF619)的方法，并验证了纯化后蛋白的体外DNA结合活性，为功能研究提供了技术参考。
**说明**：ZNF619相关研究较少，以上文献为模拟示例。建议通过PubMed或Google Scholar检索最新文献，并关注锌指蛋白家族(如ZNFs)或重组蛋白表达技术的研究拓展方向。
**Background of Recombinant Human ZNF619 Protein**
Zinc finger protein 619 (ZNF619) is a member of the C2H2 zinc finger protein family, characterized by conserved cysteine and histidine residues that coordinate zinc ions to stabilize DNA-binding domains. ZNF619 contains a canonical KRAB (Krüppel-associated box) domain at its N-terminus, which is associated with transcriptional repression through interactions with co-repressor complexes, and multiple C2H2-type zinc finger motifs at the C-terminus, enabling sequence-specific DNA or RNA binding. The protein is primarily localized to the nucleus, suggesting a role in regulating gene expression.
While the precise biological functions of ZNF619 remain under investigation, it is hypothesized to participate in epigenetic regulation, cell differentiation, and development. Emerging studies link ZNF619 to cancer pathogenesis, with altered expression observed in certain tumors, potentially influencing oncogenic or tumor-suppressive pathways. For instance, ZNF619 may regulate genes involved in cell proliferation or apoptosis, though mechanistic insights are limited.
Recombinant human ZNF619 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) for functional studies. Its production enables *in vitro* analyses, such as DNA-protein interaction assays, structural studies, and screening for potential therapeutic ligands. Current research focuses on elucidating its target genes, regulatory networks, and disease relevance, positioning ZNF619 as a candidate for biomarker discovery or therapeutic targeting in precision medicine. Further studies are required to fully define its physiological and pathological roles.
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