| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF614 |
| Uniprot No | Q8N883 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-198 aa |
| 活性数据 | MIKTQESLTLEDVAVEFSWEEWQLLDTAQKNLYRDVMVENYNHLVSLGYQTSKPDVLSKLAHGQEPWTTDAKIQNKNCPGIGKVDSHLQEHSPNQRLLKSVQQCNGQNTLRNIVHLSKTHFPIVQNHDTFDLYRKNLKSSLSLINQKRRHGINNPVEFIGETGSLERPRQADHLRSEVQDQPGQRGETLCLLKIHTKN |
| 分子量 | 49.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF614蛋白的参考文献示例(注：文献为虚构，仅用于示例，实际文献需通过学术数据库检索确认)：
1. **"Functional characterization of recombinant ZNF614 and its role in neuronal differentiation"**
- **作者**: Zhang Y, Li R, Wang X.
- **摘要**: 本研究通过在大肠杆菌中表达重组人ZNF614蛋白，分析了其与DNA结合的能力，并发现ZNF614通过调控神经分化相关基因(如NeuroD1)的启动子活性，影响神经元分化过程。
2. **"ZNF614 interacts with chromatin remodelers via its zinc finger domains"**
- **作者**: Garcia-Sanchez P, Müller H.
- **摘要**: 利用重组ZNF614蛋白进行免疫共沉淀实验，发现其与染色质重塑复合物(如SWI/SNF)的亚基存在相互作用，提示ZNF614可能通过表观遗传调控参与细胞周期进程。
3. **"Structural and functional analysis of the ZNF614 recombinant protein in cancer cell proliferation"**
- **作者**: Tanaka K, et al.
- **摘要**: 通过晶体学解析了ZNF614的锌指结构域，并结合功能实验表明，重组ZNF614在胃癌细胞中过表达会激活Wnt信号通路，促进细胞增殖和迁移。
4. **"ZNF614 knockout and recombinant rescue models reveal its essential role in embryonic development"**
- **作者**: Wei C, et al.
- **摘要**: 在小鼠胚胎干细胞中敲除ZNF614导致发育异常，而通过外源添加重组ZNF614蛋白可部分恢复中胚层分化缺陷，证实其在早期胚胎基因调控中的必要性。
---
**注意**：以上文献及摘要均为假设性示例，实际研究中请通过PubMed、Google Scholar等平台检索具体文献。若ZNF614相关研究较少，可扩展至锌指蛋白家族的其他成员(如ZNF217、ZNF384)作为参考。
**Background of Recombinant Human ZNF614 Protein**
Zinc finger protein 614 (ZNF614) is a member of the zinc finger protein family, characterized by conserved Cys₂His₂ (C2H2) zinc finger domains that mediate sequence-specific DNA or RNA binding. These proteins play critical roles in transcriptional regulation, chromatin remodeling, and cellular differentiation. ZNF614. located on human chromosome 19. is postulated to function as a transcription factor, though its precise biological mechanisms remain under investigation.
Studies suggest ZNF614 may participate in neurodevelopment, stem cell regulation, and tumorigenesis. For instance, it has been linked to neural progenitor cell proliferation and differentiation, while dysregulation is observed in certain cancers, hinting at potential oncogenic or tumor-suppressive roles. Recombinant human ZNF614 protein, engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies by providing a purified, active form for *in vitro* assays.
Researchers utilize this recombinant protein to explore DNA/RNA interactions, signaling pathways, and protein-protein networks, aiming to clarify ZNF614's role in development and disease. Its applications extend to drug discovery, particularly in targeting transcription-related disorders. Further characterization of ZNF614 could uncover novel therapeutic strategies for neurological conditions or cancers.
×
