| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF772 |
| Uniprot No | Q68DY9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-489 aa |
| 活性数据 | MAAAEPMGPAQVPMNSEVIVDPIQGQVNFEDVFVYFSQEEWVLLDEAQRLLYRDVMLENFALMASLGHTSFMSHIVASLVMGSEPWVPDWVDMTLAVATETPGGSDPGCWHGMEDEEIPFEQSFSIGMSQIRIPKGGPSTQKAYPCGTCGLVLKDILHLAEHQETHPGQKPYMCVLCGKQFCFSANLHQHQKQHSGEKPFRSDKSRPFLLNNCAVQSMEMSFVTGEACKDFLASSSIFEHHAPHNEWKPHSNTKCEEASHCGKRHYKCSECGKTFSRKDSLVQHQRVHTGERPYECGECGKTFSRKPILAQHQRIHTGEMPYECGICGKVFNHSSNLIVHQRVHTGARPYKCSECGKAYSHKSTLVQHESIHTGERPYECSECGKYFGHKYRLIKHWSVHTGARPYECIACGKFFSQSSDLIAHQRVHNGEKPYVCSECGKAFSHKHVLVQHHRIHTGERPYKCSECGKAFRQRASLIRHWKIHTGERP |
| 分子量 | 80.19 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF772蛋白的3篇模拟参考文献(实际文献可能需要通过学术数据库验证)：
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1. **标题**: *"Cloning and functional characterization of human ZNF772. a novel KRAB-zinc finger protein"*
**作者**: Zhang L, et al.
**摘要**: 本研究首次报道了人源ZNF772基因的克隆及其重组蛋白在大肠杆菌中的表达。通过体外实验验证了ZNF772蛋白通过锌指结构域与特定DNA序列结合的能力，并发现其可能参与调控细胞增殖相关基因。
2. **标题**: *"ZNF772 regulates epigenetic silencing in cancer via interaction with KRAB-associated protein-1 (KAP1)"*
**作者**: Wang Y, et al.
**摘要**: 文章利用重组ZNF772蛋白进行免疫共沉淀和染色质免疫沉淀(ChIP)实验，发现其通过与KAP1蛋白相互作用介导组蛋白修饰，进而抑制肿瘤抑制基因的转录，为癌症表观遗传调控提供新机制。
3. **标题**: *"Structural insights into the DNA-binding specificity of ZNF772 zinc finger domains"*
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析重组ZNF772蛋白的锌指结构域三维结构，揭示了其与靶DNA结合的分子基础，并利用点突变实验证明关键氨基酸残基对DNA结合特异性的贡献。
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**说明**：上述文献为模拟内容，真实研究可能需要检索PubMed、Web of Science或Google Scholar等平台(关键词：ZNF772. KRAB-ZFP, recombinant protein)。若需实际文献，建议补充具体研究背景或联系领域数据库。
**Recombinant human ZNF772 protein** is a genetically engineered version of the zinc finger protein 772 (ZNF772), encoded by the *ZNF772* gene in humans. Zinc finger proteins (ZFPs) are a large family of transcription factors characterized by tandem Cys2-His2 (C2H2) zinc finger domains, which facilitate sequence-specific DNA, RNA, or protein interactions. ZNF772. a member of the Krüppel-associated box (KRAB)-ZFPs subfamily, contains multiple C2H2 zinc finger motifs and a KRAB domain, suggesting roles in transcriptional regulation, chromatin remodeling, or epigenetic silencing. While the precise biological functions of ZNF772 remain understudied, its structural features imply involvement in binding regulatory DNA elements, potentially modulating gene expression during development, differentiation, or stress responses. Recombinant ZNF772 is typically produced in heterologous systems (e.g., *E. coli* or mammalian cells*) to enable functional studies, such as elucidating its DNA-binding specificity, interaction partners, or regulatory pathways. Interest in ZNF772 also stems from its potential links to diseases, including cancer, where aberrant expression of KRAB-ZFPs may contribute to oncogenesis or tumor suppression. Advances in recombinant protein technology have facilitated the production of high-purity, bioactive ZNF772 for structural analysis, *in vitro* assays, and therapeutic exploration, offering insights into its molecular mechanisms and therapeutic relevance. Further research is needed to clarify its physiological and pathological significance.
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