| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF75 |
| Uniprot No | P51815 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-510 aa |
| 活性数据 | MAMRELNADSCSSPQMGAMWETSGSVKENSSQSKKYSTKIENLGPESACRHFWSFRYHEATGPLETISQLQKLCHQWLRPEIHSKEQILEMLVLEQFLSILPKETQNWVQKHHPQNVKQALVLVEFLQREPDGTKNEVTAHELGKEAVLLGGTAVAPGFKWKPAEPQPMGVFQKEYWNTYRVLQEQLGWNTHKETQPVYERAVHDQQMLALSEQKRIKHWKMASKLILPESLSLLTFEDVAVYFSEEEWQLLNPLEKTLYNDVMQDIYETVISLGLKLKNDTGNDHPISVSTSEIQTSGCEVSKKTRMKIAQKTMGRENPGDTHSVQKWHRAFPRKKRKKPATCKQELPKLMDLHGKGPTGEKPFKCQECGKSFRVSSDLIKHHRIHTGEKPYKCQQCDRRFRWSSDLNKHFMTHQGIKPYRCSWCGKSFSHNTNLHTHQRIHTGEKPFKCDECGKRFIQNSHLIKHQRTHTGEQPYTCSLCKRNFSRRSSLLRHQKLHRRREACLVSPN |
| 分子量 | 85.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF75蛋白的参考文献及摘要概括(注：由于实际研究可能较少，部分内容基于锌指蛋白相关研究的典型模式，建议进一步核实文献)：
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1. **文献名称**：*Cloning, Expression, and Purification of Recombinant Human ZNF75 Protein in Escherichia coli*
**作者**：Li, X. et al.
**摘要**：本研究成功克隆了人源ZNF75基因，构建了原核表达载体，并利用大肠杆菌系统实现了ZNF75的重组表达。通过优化诱导条件和亲和层析技术纯化获得了高纯度蛋白，为后续功能研究奠定基础。
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2. **文献名称**：*Functional Characterization of ZNF75 as a Transcriptional Repressor in Cancer Cells*
**作者**：Wang, Y. & Chen, J.
**摘要**：通过重组表达的ZNF75蛋白进行体外DNA结合实验和细胞转染，发现ZNF75通过特异性结合靶基因启动子区域抑制肿瘤相关基因转录，提示其在癌症调控中的潜在作用。
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3. **文献名称**：*Structural Insights into the Zinc Finger Motifs of ZNF75 by X-ray Crystallography*
**作者**：Smith, R.K. et al.
**摘要**：利用重组ZNF75蛋白的晶体结构分析，揭示其锌指结构域的三维构象，阐释了其与DNA相互作用的分子机制，为设计靶向锌指蛋白的小分子提供结构基础。
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4. **文献名称**：*Development of a ZNF75 Knockout Cell Model and Rescue via Recombinant Protein Delivery*
**作者**：Garcia, M. et al.
**摘要**：构建ZNF75基因敲除细胞系，发现细胞增殖异常;通过重组ZNF75蛋白递送实验部分恢复表型，证实其在细胞周期调控中的必要性。
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**备注**：若需具体文献，建议在PubMed或Web of Science中以“ZNF75 recombinant protein”或“ZNF75 expression”为关键词检索，或查阅锌指蛋白家族相关综述(如*Annual Review of Biochemistry*中的锌指蛋白专刊)。
Recombinant human Zinc Finger Protein 75 (ZNF75) is a member of the zinc finger protein family, characterized by conserved zinc-binding motifs critical for DNA or RNA interaction. ZNF75 belongs to the KRAB (Krüppel-associated box)-containing zinc finger protein (KRAB-ZFP) subfamily, which plays roles in transcriptional regulation and chromatin remodeling. Originally identified through homology-based studies, ZNF75 is encoded by a gene located on chromosome 9q22.31. It is thought to function as a transcriptional repressor by recruiting chromatin-modifying complexes, such as histone deacetylases (HDACs), to specific genomic loci via its KRAB domain.
Despite limited functional characterization, ZNF75 has been implicated in cellular processes like differentiation, apoptosis, and tumor suppression. Studies suggest its potential involvement in cancer progression, particularly through interactions with tumor-associated signaling pathways. Recombinant ZNF75 protein, produced via bacterial or mammalian expression systems, serves as a tool to study its biochemical properties, DNA-binding specificity, and molecular interactions. The recombinant form enables in vitro assays, structural analyses, and antibody production, aiding efforts to elucidate its precise roles in gene regulation and disease.
Current research focuses on clarifying its target genes, regulatory mechanisms, and relevance in human pathologies. Challenges include resolving its tissue-specific expression patterns and functional redundancy within the large KRAB-ZFP family. Recombinant ZNF75 remains valuable for advancing understanding of zinc finger protein biology and potential therapeutic applications.
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