| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF84 |
| Uniprot No | P51523 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-738 aa |
| 活性数据 | MTMLQESFSFDDLSVDFTQKEWQLLDPSQKNLYKDVMLENYSSLVSLGYEVMKPDVIFKLEQGEEPWVGDGEIPSSDSPEVWKVDGNMMWHQDNQDKLKIIKRGHECDAFGKNFNLNMNFVPLRKSNSEGDLDGLILKHHLDLLIPKGDYGKAESDDFNVFDNFFLHSKPEDTDTWLKYYDCDKYKESYKKSQIIIYHRNRLGEKLYECSECRKRFSKKPSLIKHQSRHIRDIAFGCGNCGKTFPQKSQFITHHRTHTGEKPYNCSQCGKAFSQKSQLTSHQRTHTGEKPYECGECGKAFSRKSHLISHWRTHTGEKPYGCNECGRAFSEKSNLINHQRIHTGEKPFECRECGKAFSRKSQLVTHHRTHTGTKPFGCSDCRKAFFEKSELIRHQTIHTGEKPYECSECRKAFRERSSLINHQRTHTGEKPHGCIQCGKAFSQKSHLISHQMTHTGEKPFICSKCGKAFSRKSQLVRHQRTHTGEKPYECSECGKAFSEKLSLTNHQRIHTGEKPYVCSECGKAFCQKSHLISHQRTHTGEKPYECSECGKAFGEKSSLATHQRTHTGEKPYECRDCEKAFSQKSQLNTHQRIHTGEKPYECSLCRKAFFEKSELIRHLRTHTGEKPYECNECRKAFREKSSLINHQRIHTGEKPFECSECGKAFSRKSHLIPHQRTHTGEKPYGCSECRKAFSQKSQLVNHQRIHTGEKPYRCIECGKAFSQKSQLINHQRTHTVKKS |
| 分子量 | 111.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF84蛋白的假设性参考文献示例。由于实际文献可能有限，以下内容仅供参考：
1. **"Structural Insights into the DNA-Binding Mechanism of Human ZNF84"**
*Author: Smith J, et al. (2012)*
摘要：本研究通过重组表达纯化人源ZNF84蛋白，利用X射线晶体学解析其结构，揭示了锌指结构域与特定DNA序列的结合模式，为理解其基因调控机制提供结构基础。
2. **"Recombinant ZNF84 as a Transcriptional Repressor in Cellular Differentiation"**
*Author: Lee H, et al. (2016)*
摘要：在大肠杆菌中成功表达重组人ZNF84蛋白，并发现其通过结合启动子区域抑制靶基因表达，提示其在干细胞分化中的潜在调控作用。
3. **"Expression Optimization and Functional Analysis of Recombinant ZNF84 in Cancer Models"**
*Author: Chen W, et al. (2019)*
摘要：优化了哺乳动物细胞中重组ZNF84蛋白的表达体系，证明其与表观修饰复合物相互作用，可能通过调控肿瘤相关基因影响癌细胞增殖。
4. **"A Novel Protocol for High-Yield Purification of Recombinant ZNF84 for Biophysical Studies"**
*Author: Zhang Y, et al. (2021)*
摘要：开发了基于亲和层析的重组ZNF84高效纯化方法，获得高纯度蛋白用于体外DNA结合动力学分析，揭示了其独特的结合亲和力特征。
**注意**：以上文献为示例性质，实际研究中关于ZNF84的公开数据可能较少。建议通过数据库(如PubMed、Google Scholar)使用关键词“ZNF84 recombinant”或“ZNF84 protein”核实最新进展。若研究较少，可扩展至锌指蛋白家族或同源蛋白的相关文献。
The recombinant human ZNF84 protein is a engineered version of the zinc finger protein 84 (ZNF84), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (ZFP) family. These proteins are characterized by tandem C2H2-type zinc finger motifs that enable sequence-specific DNA binding, coupled with a KRAB domain that typically mediates transcriptional repression through interaction with co-repressors. ZNF84 is thought to play roles in gene regulation, particularly in developmental processes and cellular differentiation, though its precise biological functions remain less characterized compared to other ZFPs.
Recombinant ZNF84 is commonly produced in heterologous expression systems, such as Escherichia coli or mammalian cell cultures, to enable biochemical and functional studies. The recombinant protein allows researchers to investigate its DNA-binding specificity, interaction partners, and regulatory mechanisms in vitro or in cellular models. Due to the modular nature of zinc finger proteins, ZNF84 has attracted interest in synthetic biology and genome-editing applications, where engineered zinc finger arrays are utilized for targeted gene modulation. Studies have also explored its potential involvement in diseases, including cancer and genetic disorders linked to chromosomal instability. Ongoing research aims to unravel its physiological targets and therapeutic relevance, bridging structural insights with functional genomics.
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