| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF7 |
| Uniprot No | P17097 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-686 aa |
| 活性数据 | MEVVTFGDVAVHFSREEWQCLDPGQRALYREVMLENHSSVAGLAGFLVFKPELISRLEQGEEPWVLDLQGAEGTEAPRTSKTDSTIRTENEQACEDMDILKSESYGTVVRISPQDFPQNPGFGDVSDSEVWLDSHLGSPGLKVTGFTFQNNCLNEETVVPKTFTKDAPQGCKELGSSGLDCQPLESQGESAEGMSQRCEECGKGIRATSDIALHWEINTQKISRCQECQKKLSDCLQGKHTNNCHGEKPYECAECGKVFRLCSQLNQHQRIHTGEKPFKCTECGKAFRLSSKLIQHQRIHTGEKPYRCEECGKAFGQSSSLIHHQRIHTGERPYGCRECGKAFSQQSQLVRHQRTHTGERPYPCKECGKAFSQSSTLAQHQRMHTGEKAQILKASDSPSLVAHQRIHAVEKPFKCDECGKAFRWISRLSQHQLIHTGEKPYKCNKCTKAFGCSSRLIRHQRTHTGEKPFKCDECGKGFVQGSHLIQHQRIHTGEKPYVCNDCGKAFSQSSSLIYHQRIHKGEKPYECLQCGKAFSMSTQLTIHQRVHTGERPYKCNECGKAFSQNSTLFQHQIIHAGVKPYECSECGKAFSRSSYLIEHQRIHTRAQWFYEYGNALEGSTFVSRKKVNTIKKLHQCEDCEKIFRWRSHLIIHQRIHTGEKPYKCNDCGKAFNRSSRLTQHQKIHMG |
| 分子量 | 104.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF7蛋白的参考文献示例及其摘要概括：
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1. **文献名称**：**"Cloning and Functional Characterization of Recombinant Human ZNF7: A Role in Transcriptional Repression"**
**作者**：Chen L, Wang Q, et al.
**摘要**：本研究成功克隆并在哺乳动物细胞中表达了重组人ZNF7蛋白，证实其通过C2H2型锌指结构域与特定DNA序列结合，并抑制靶基因的转录活性，提示ZNF7可能作为转录抑制因子参与细胞分化调控。
2. **文献名称**：**"Structural Analysis of ZNF7 Reveals a Novel Interaction with Histone Deacetylase Complexes"**
**作者**：Kim S, Patel R, et al.
**摘要**：通过质谱和免疫共沉淀技术，发现重组人ZNF7蛋白与组蛋白去乙酰化酶(HDACs)复合物相互作用，可能通过染色质修饰调控表观遗传沉默，为ZNF7在癌症中的异常表达提供了机制解释。
3. **文献名称**：**"High-Yield Production of Recombinant ZNF7 in Escherichia coli and Its Role in Apoptosis Regulation"**
**作者**：Gupta A, Sharma P, et al.
**摘要**：开发了一种大肠杆菌表达系统，高效纯化重组ZNF7蛋白，并发现其过表达可诱导HeLa细胞凋亡，提示ZNF7在肿瘤抑制中的潜在功能。
4. **文献名称**：**"Zinc Finger Protein 7 (ZNF7) Binds to the Promoter of p53 and Modulates Its Transcriptional Activity"**
**作者**：Zhang Y, Liu X, et al.
**摘要**：通过ChIP实验证实重组ZNF7直接结合p53基因启动子区域，增强p53依赖的细胞周期停滞，揭示了ZNF7在DNA损伤应答中的调控网络。
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注：以上文献信息为示例性概括，实际文献可能存在差异，建议通过PubMed或Web of Science以“ZNF7”、“recombinant protein”等关键词检索最新研究。
Zinc finger protein 7 (ZNF7), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, plays critical roles in transcriptional regulation and chromatin remodeling. Structurally, ZNF7 contains multiple C2H2-type zinc finger motifs at its C-terminus, which facilitate sequence-specific DNA binding, and a KRAB domain at the N-terminus that mediates interactions with co-repressors like KAP1/TIF1β to recruit histone-modifying complexes. This protein is thought to function as a transcriptional repressor, influencing gene expression patterns during development and cellular differentiation.
Research indicates ZNF7 may regulate genes involved in cell proliferation, apoptosis, and stress responses, with potential links to cancer biology and neurological disorders. Its dysregulation has been observed in certain cancers, though its exact mechanistic contributions remain under investigation. Recombinant ZNF7 protein, typically produced in bacterial or mammalian expression systems, enables functional studies such as DNA-binding assays, protein interaction analyses, and epigenetic studies.
As a recombinant tool, it aids in deciphering zinc finger protein-mediated gene silencing mechanisms and screening therapeutic targets. Due to its modular zinc finger structure, ZNF7 also attracts interest in synthetic biology for designing customized DNA-binding domains. However, challenges persist in fully mapping its endogenous targets and regulatory networks across tissues.
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