| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF821 |
| Uniprot No | O75541 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-370 aa |
| 活性数据 | MSRRKQTNPNKVHCDSEGDEEETTQDEVSSHTSEEDGGVVKVEKELENTEQPVGGNEVVEHEVTGNLNSDPLLELCQCPLCQLDCGSREQLIAHVYQHTAAVVSAKSYMCPVCGRALSSPGSLGRHLLIHSEDQRSNCAVCGARFTSHATFNSEKLPEVLNMESLPTVHNEGPSSAEGKDIAFSPPVYPAGILLVCNNCAAYRKLLEAQTPSVRKWALRRQNEPLEVRLQRLERERTAKKSRRDNETPEEREVRRMRDREAKRLQRMQETDEQRARRLQRDREAMRLKRANETPEKRQARLIREREAKRLKRRLEKMDMMLRAQFGQDPSAMAALAAEMNFFQLPVSGVELDSQLLGKMAFEEQNSSSLH |
| 分子量 | 68.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人ZNF821蛋白的参考示例文献(注：文献为假设性示例，建议通过学术数据库检索真实文献)：
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1. **文献名称**: *Cloning, Expression, and Purification of Recombinant Human ZNF821 Protein in E. coli*
**作者**: Zhang L, et al.
**摘要**: 本研究成功克隆了人ZNF821基因，并利用大肠杆菌表达系统高效表达该蛋白。通过镍柱亲和层析纯化得到高纯度重组蛋白，Western blot验证其抗原性，为后续功能研究奠定基础。
2. **文献名称**: *ZNF821 as a Tumor Suppressor: Functional Analysis of Recombinant Protein in Breast Cancer Cells*
**作者**: Wang Y, et al.
**摘要**: 研究通过真核系统表达重组ZNF821蛋白，发现其显著抑制乳腺癌细胞增殖和迁移。机制研究表明，ZNF821通过调控p53通路诱导细胞周期阻滞，提示其潜在抑癌作用。
3. **文献名称**: *Structural Characterization of Recombinant ZNF821 via Cryo-EM*
**作者**: Li X, et al.
**摘要**: 利用冷冻电镜技术解析重组ZNF821蛋白的三维结构，揭示其锌指结构域与DNA结合的分子机制，为理解其基因调控功能提供结构基础。
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**建议**：实际研究中，可通过PubMed、Web of Science等平台，以“ZNF821 recombinant protein”、“ZNF821 functional study”为关键词检索最新文献。关注实验方法(如表达系统、功能验证)及疾病关联(如癌症、神经退行性疾病)的研究方向。
Zinc finger protein 821 (ZNF821) is a member of the zinc finger protein family, characterized by conserved Cys₂His₂ (C2H2)-type zinc finger domains that mediate sequence-specific DNA binding or protein-protein interactions. As a transcription regulator, ZNF821 is predominantly localized in the nucleus and is hypothesized to participate in transcriptional control, though its precise mechanistic roles remain under investigation. The protein is encoded by the ZNF821 gene located on human chromosome 16q22.1. a region occasionally linked to genetic disorders and cancers. Structurally, it contains multiple zinc finger motifs clustered at its C-terminal region, a feature common in proteins involved in nucleic acid recognition and gene regulation.
Studies suggest ZNF821 may influence cellular processes such as differentiation, proliferation, and apoptosis. Notably, it has been associated with tumorigenesis in certain cancers, though its role as an oncogene or tumor suppressor appears context-dependent. For instance, reduced ZNF821 expression correlates with poor prognosis in hepatocellular carcinoma, implying potential tumor-suppressive functions. In neurodegenerative contexts, ZNF821 interacts with pathways involved in neuronal survival, though detailed mechanisms require further validation. Recombinant ZNF821 protein, produced via bacterial or mammalian expression systems, enables functional studies, antibody development, and screening for molecular partners. Current research focuses on delineating its target genes, regulatory networks, and therapeutic potential in diseases linked to transcriptional dysregulation. Despite progress, ZNF821's full biological significance and pathophysiological relevance remain areas of active exploration.
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