纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | ZNF81 |
Uniprot No | P51508 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-122 aa |
活性数据 | MPANEDAPQPGEHGSACEVSVSFEDVTVDFSREEWQQLDSTQRRLYQDVMLENYSHLLSVGFEVPKPEVIFKLEQGEGPWTLEGEAPHQSCSDGKFGIKPSQRRISGKSTFHSEMEGEDTLC |
分子量 | 40.1 kDa |
蛋白标签 | GST-tag at N-terminal |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF81蛋白的3篇假设参考文献(注:文献为假设示例,实际研究中请核实真实文献):
1. **《Expression and Purification of Recombinant Human ZNF81 in a Bacterial System》**
- 作者:Smith J, et al.
- 摘要:报道了在大肠杆菌中成功表达带有His标签的重组人ZNF81蛋白,通过镍柱亲和层析纯化,并通过质谱验证其正确性,为后续功能研究提供材料。
2. **《ZNF81 Recombinant Protein Inhibits Tumor Cell Proliferation via DNA Binding Activity》**
- 作者:Lee S, et al.
- 摘要:研究显示,利用昆虫细胞表达系统制备的重组ZNF81蛋白能特异性结合靶基因启动子区域,并在体外抑制多种癌细胞的生长。
3. **《Structural and Functional Analysis of Zinc Finger Domain in Recombinant ZNF81》**
- 作者:Wang Y, et al.
- 摘要:通过定点突变和圆二色谱分析重组ZNF81蛋白的锌指结构域,证实其锌离子结合能力对维持DNA结合活性至关重要。
建议在实际数据库中检索时结合关键词 **"recombinant ZNF81"、"ZNF81 protein purification"** 或 **"ZNF81 functional study"** 筛选文献。
ZNF81. a member of the zinc finger protein (ZFP) family, is a transcription factor characterized by conserved Cys2/His2-type zinc finger motifs. These motifs enable sequence-specific DNA binding, facilitating its role in transcriptional regulation. Located on the X chromosome, ZNF81 is ubiquitously expressed, with notable activity in brain, testes, and immune tissues. It shares structural homology with other KRAB-ZFPs, containing a Krüppel-associated box (KRAB) domain implicated in protein-protein interactions and transcriptional repression. Dysregulation of ZNF81 has been linked to neurodevelopmental disorders, including X-linked intellectual disability and autism spectrum disorders, suggesting its critical role in neural differentiation and synaptic function.
Recombinant human ZNF81 protein is typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. Its engineered versions often include purification tags (His, GST) and optimized solubility features. Current research focuses on characterizing its DNA-binding specificity, interactome mapping with chromatin modifiers, and elucidating mechanisms in gene silencing. Recombinant ZNF81 serves as a vital tool for in vitro assays, including electrophoretic mobility shift assays (EMSAs) and high-throughput screening for potential therapeutic modulators. Structural studies using recombinant protein aim to inform drug design targeting ZNF81-associated pathologies while minimizing off-target effects on related ZFPs.
×