关键信息
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基因名
CSNK1G1
- 应用
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别名
9130020E21Rik; Casein kinase 1 gamma 1; Casein kinase I isoform gamma 1; Casein kinase I isoform gamma-1; CKI gamma 1 ; CKI-gamma 1; CSNK1G1; EC 2.7.11.1; KC1G1_HUMAN
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9HCP0
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表达区间
1-422aa
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氨基酸序列
MDHPSREKDE RQRTTKPMAQ RSAHCSRPSG SSSSSGVLMV GPNFRVGKKI GCGNFGELRL GKNLYTNEYV AIKLEPIKSR APQLHLEYRF YKQLGSAGEG LPQVYYFGPC GKYNAMVLEL LGPSLEDLFD LCDRTFTLKT VLMIAIQLLS RMEYVHSKNL IYRDVKPENF LIGRQGNKKE HVIHIIDFGL AKEYIDPETK KHIPYREHKS LTGTARYMSI NTHLGKEQSR RDDLEALGHM FMYFLRGSLP WQGLKADTLK ERYQKIGDTK RNTPIEALCE NFPEEMATYL RYVRRLDFFE KPDYEYLRTL FTDLFEKKGY TFDYAYDWVG RPIPTPVGSV HVDSGASAIT RESHTHRDRP SQQQPLRNQV VSSTNGELNV DDPTGAHSNA PITAHAEVEV VEEAKCCCFF KRKRKKTAQR HK
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分子量
48.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CSNK1G1, or Casein Kinase 1 Gamma 1, is a serine/threonine kinase that plays a critical role in various cellular processes, including cell signaling, circadian rhythm regulation, and development. Its involvement in the Wnt signaling pathway and the regulation of the cell cycle highlights its significance in both normal physiology and disease contexts, particularly in cancer. The study of CSNK1G1 has garnered attention due to its potential as a therapeutic target; dysregulation of this kinase has been implicated in several malignancies where it may contribute to tumor progression and resistance to treatments. Recombinant CSNK1G1 protein production is essential for detailed biochemical and structural studies, allowing researchers to elucidate its functional mechanisms and interactions with other proteins. Understanding the structure-function relationship of CSNK1G1 through recombinant techniques can aid in the development of inhibitors that modulate its activity, presenting new avenues for targeted cancer therapies. Furthermore, the analysis of this kinase can provide insights into its role in circadian regulation and how its dysregulation might lead to metabolic disorders. As a result, the research surrounding CSNK1G1 and its recombinant protein forms represents a burgeoning field with significant implications for both basic science and clinical applications.
