| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF843 |
| Uniprot No | Q8N446 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-348 aa |
| 活性数据 | MRSLPFALTVESVSARAPTCCSTGRFTQGRQPCKCKACGRGFTQSASLLQHWRVHSDWRETLSLSPVRQDLLWPLQPHQAPASPLGRSHSSAGVRQGFSGQLCCWLTKEHTLAEALRLSPVPAGFWGPVEADRPPANSHRRVCPFCCCSCGDSVNEKTSLSQRVLPHPGEKTCRGGSVESVSLAPSSVAPDSTSGLRPCGSPGSFLQHLPPSTLLPRPPFLYPGPPLSLQPLVPSGLPAVPAVPLGGLEVAQVPPATQPAAQQEGAMGPRSCASAGRDSREAVQAPGYPEPARKASQHRAAGPLGEARARLQRQCRAPPPPSNPHWRGALPVFPVWKASSRRSNLARH |
| 分子量 | 38.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF843蛋白的3条模拟参考文献示例(注：ZNF843研究较少，以下内容为合理虚构，建议通过学术数据库核实最新文献)：
1. **标题**: *Structural characterization of recombinant human ZNF843 protein and its DNA-binding properties*
**作者**: Li X, et al.
**摘要**: 通过X射线晶体学解析了ZNF843蛋白的锌指结构域三维结构，发现其与特定DNA序列(5'-GCCAC-3')存在强结合能力，揭示了该蛋白参与转录调控的结构基础。
2. **标题**: *ZNF843 functions as a tumor suppressor in hepatocellular carcinoma through epigenetic regulation*
**作者**: Wang Y, et al.
**摘要**: 通过CRISPR/Cas9基因敲除实验，证明ZNF843能够结合CDKN1A/p21启动子区，招募组蛋白去乙酰化酶HDAC3.抑制肝癌细胞增殖。重组ZNF843蛋白在体外可恢复肿瘤细胞的周期阻滞。
3. **标题**: *Recombinant ZNF843 protein expression system optimization and functional validation*
**作者**: Zhang L, et al.
**摘要**: 在昆虫细胞系统中实现了ZNF843的重组表达，优化获得纯度>95%的蛋白。表面等离子共振(SPR)实验证实其与靶基因启动子的解离常数Kd=28 nM，为后续分子机制研究提供工具蛋白。
建议通过PubMed搜索最新文献(关键词：ZNF843 protein recombinant 或 zinc finger protein 843)，关注其与神经系统发育或癌症相关的最新功能研究。
**Background of Recombinant Human ZNF843 Protein**
Zinc finger protein 843 (ZNF843), a member of the zinc finger (ZNF) family, is characterized by its evolutionarily conserved Cys₂His₂ (C2H2) domains, which enable sequence-specific DNA or RNA binding. This protein is implicated in transcriptional regulation, though its precise biological roles remain under investigation. ZNF843 is expressed in various tissues, with studies suggesting potential involvement in cellular processes such as differentiation, development, and responses to cellular stress. Dysregulation of zinc finger proteins, including ZNF843. has been linked to cancers, neurological disorders, and immune diseases, highlighting its broader relevance in human health.
Recombinant human ZNF843 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form for experimental applications. It typically retains the DNA-binding zinc finger motifs and may include affinity tags (e.g., His-tag) for simplified purification and detection. Research tools like recombinant ZNF843 enable *in vitro* studies to map DNA/RNA interactions, identify target genes, and explore its regulatory mechanisms. Additionally, it aids in structural analyses (e.g., X-ray crystallography) and screens for therapeutic compounds modulating ZNF843 activity. Current efforts focus on elucidating its role in gene networks and disease contexts, positioning it as a potential target for precision medicine strategies.
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