| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF85 |
| Uniprot No | Q03923 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-536 aa |
| 活性数据 | MSLSAQPSSSFYKPVSEVMCSHFAQDLWPEQNIKDSFQKVTLKRYGKCRHENLPLRKGCESMDECKMHKGGCNGLNQCLTATQSKIFQCDKYVKVAHKFSNSNRHEIRHTKKKPFKCTKCGKSFGMISCLTEHSRIHTRVNFYKCEECGKAFNWSSTLTKHKRIHTGEKPYKCEECGKAFNQSSNLIKHKKIHTGEKPYKCEECGKTFNRFSTLTTHKIIHTGEKPYKCKECGKAFNRSSTLTTHRKIHTGEKPYKCEECGKAFKQSSNLTTHKIIHTGEKPYKCKKCGKAFNQSAHLTTHEVIHTGEKPYKCEKCGKAFNHFSHLTTHKIIHTGEKPYKCKECGKAFKHSSTLTKHKIIHTGEKPYKCKECEKAFNQSSKLTEHKKIHTGEKPYECEKCGKAFNQSSNLTRHKKSHTEEKPYKCEECGKGFKWPSTLTIHKIIHTGEKPYKCEECGKAFNQSSKLTKHKKIHTGEKPYTCEECGKAFNQSSNLTKHKRIHTGEKPYKCEECDKAFKWSSVLTKHKIIHTGEKLQI |
| 分子量 | 88.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF85蛋白的3篇参考文献概览(文献名称、作者、摘要内容简写)：
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1. **"Functional characterization of the human zinc finger protein ZNF85 in transcriptional regulation"**
- **作者**: Chen L, Wang Y, Zhang H.
- **摘要**: 研究利用重组ZNF85蛋白，揭示其通过与特定DNA序列结合调控基因转录的功能，并证明其在细胞分化中的潜在作用。
2. **"Expression and purification of recombinant human ZNF85 for structural analysis"**
- **作者**: Kim S, Park JH, Lee M.
- **摘要**: 开发了一种高效的大肠杆菌重组表达系统纯化ZNF85蛋白的方法，并通过X射线晶体学分析其锌指结构域的构象特征。
3. **"ZNF85 interacts with viral proteins to modulate host immune responses"**
- **作者**: Gupta R, Kumar A, Singh S.
- **摘要**: 发现重组ZNF85蛋白可与某些病毒蛋白相互作用，抑制病毒复制并增强宿主天然免疫信号通路，提示其在抗病毒治疗中的潜在应用。
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注：以上文献为示例，具体研究需通过学术数据库(如PubMed或Google Scholar)检索实际存在的论文。建议结合关键词“ZNF85 recombinant protein”“ZNF85 function”进行筛选。
Zinc finger protein 85 (ZNF85), a member of the Kruppel-associated box (KRAB) domain-containing zinc finger protein family, is encoded by the *ZNF85* gene in humans. Zinc finger proteins are characterized by tandem repeats of conserved cysteine and histidine residues that form structural motifs capable of binding nucleic acids or proteins. ZNF85 contains multiple C2H2-type zinc finger domains at its C-terminus, which are typically involved in sequence-specific DNA recognition, and a KRAB domain at the N-terminus known to mediate protein-protein interactions and transcriptional repression.
As a transcriptional regulator, ZNF85 is postulated to play roles in chromatin remodeling, gene silencing, and developmental processes. It is predominantly expressed in immune tissues and reproductive organs, suggesting potential involvement in immune response modulation or gametogenesis. Studies link zinc finger proteins to epigenetic regulation, and ZNF85 may interact with repressor complexes through its KRAB domain to suppress transposable elements or regulate specific gene networks.
Recombinant human ZNF85 protein, produced via expression systems like *E. coli* or mammalian cells, enables functional studies, including DNA-binding assays, protein interaction analyses, and mechanistic investigations of its regulatory roles. Despite limited direct characterization, its structural homology with other KRAB-ZNFs highlights its importance in genome stability and disease pathways, particularly cancer and autoimmune disorders. Further research is needed to elucidate its precise biological targets and therapeutic potential.
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