| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF92 |
| Uniprot No | Q03936 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-586 aa |
| 活性数据 | MGPLTFRDVKIEFSLEEWQCLDTAQRNLYRDVMLENYRNLVFLGIAVSKPDLITWLEQGKEPWNLKRHEMVDKTPVMCSHFAQDVWPEHSIKDSFQKVILRTYGKYGHENLQLRKDHKSVDACKVYKGGYNGLNQCLTTTDSKIFQCDKYVKVFHKFPNVNRNKIRHTGKKPFKCKNRGKSFCMLSQLTQHKKIHTREYSYKCEECGKAFNWSSTLTKHKIIHTGEKPYKCEECGKAFNRSSNLTKHKIIHTGEKPYKCEECGKAFNRSSTLTKHKRIHTEEKPYKCEECGKAFNQFSILNKHKRIHMEDKPYKCEECGKAFRVFSILKKHKIIHTGEKPYKCEECGKAFNQFSNLTKHKIIHTGEKPYKCDECGKAFNQSSTLTKHKRIHTGEKPYKCEECGKAFKQSSTLTEHKIIHTGEKPYKCEKCGKAFSWSSAFTKHKRNHMEDKPYKCEECGKAFSVFSTLTKHKIIHTREKPYKCEECGKAFNQSSIFTKHKIIHTEGKSYKCEKCGNAFNQSSNLTARKIIYTGEKPYKYEECDKAFNKFSTLITHQIIYTGEKPCKHECGRAFNKSSNYTKEKLQT |
| 分子量 | 94.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF92蛋白的参考文献示例(内容基于研究领域常见方向模拟，建议通过学术数据库核实具体文献)：
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1. **文献名称**: *ZNF92 as a Novel Transcriptional Regulator in Human Cells*
**作者**: Li, X., et al.
**摘要**: 本研究成功在大肠杆菌中重组表达了人源ZNF92蛋白，并验证其与特定DNA序列的体外结合能力。通过ChIP-seq分析，发现ZNF92在基因启动子区域富集，提示其可能在调控细胞增殖相关基因中发挥作用。
2. **文献名称**: *Structural and Functional Analysis of Recombinant ZNF92 Zinc Finger Domains*
**作者**: Zhang, Y., et al.
**摘要**: 利用哺乳动物表达系统纯化重组ZNF92蛋白，结合X射线晶体学解析其锌指结构域的三维结构。功能实验表明，突变特定氨基酸残基会显著降低ZNF92对靶基因的转录抑制活性。
3. **文献名称**: *ZNF92 Interaction with HIV-1 Viral Proteins: Implications for Antiviral Response*
**作者**: Wang, H., et al.
**摘要**: 通过体外重组表达ZNF92蛋白，证实其与HIV-1衣壳蛋白p24的相互作用。研究发现ZNF92可能通过干扰病毒颗粒组装抑制HIV复制，为抗病毒治疗提供新靶点。
4. **文献名称**: *Role of Recombinant ZNF92 in Epigenetic Modifications During Cancer Development*
**作者**: Gupta, R., et al.
**摘要**: 研究报道了重组ZNF92蛋白在癌症细胞中与组蛋白去乙酰化酶(HDAC)复合物的协同作用，揭示其通过调控染色质结构影响肿瘤相关基因的表达模式。
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**说明**：上述内容为领域内研究方向的概括性模拟，具体文献需通过PubMed、Web of Science等平台检索关键词“ZNF92”“recombinant protein”“zinc finger protein 92”获取。
**Background of Recombinant Human ZNF92 Protein**
Zinc Finger Protein 92 (ZNF92), a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, is encoded by the *ZNF92* gene in humans. This protein is characterized by tandem C₂H₂-type zinc finger domains, which enable sequence-specific DNA or RNA binding, and a KRAB domain that typically mediates transcriptional repression via interaction with co-repressors. ZNF92 is hypothesized to play roles in regulating gene expression, cell differentiation, and developmental processes, though its precise biological functions remain understudied compared to other zinc finger proteins.
Recombinant human ZNF92 protein is engineered using heterologous expression systems (e.g., bacterial, insect, or mammalian cells) to produce purified, functional protein for research. Its recombinant form allows researchers to investigate DNA/RNA-binding specificity, protein-protein interactions, and regulatory mechanisms in vitro or in cellular models. Studies suggest ZNF92 may influence chromatin remodeling, participate in antiviral defense, or contribute to cancer progression, though mechanistic insights are limited. Current applications include exploring its role in epigenetic silencing, viral restriction, or as a potential biomarker. However, challenges persist in mapping its full target spectrum and understanding its context-dependent functions. Recombinant ZNF92 serves as a critical tool for unraveling its molecular biology and therapeutic potential.
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