| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF8 |
| Uniprot No | P17098 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-575 aa |
| 活性数据 | MDPEDEGVAGVMSVGPPAARLQEPVTFRDVAVDFTQEEWGQLDPTQRILYRDVMLETFGHLLSIGPELPKPEVISQLEQGTELWVAERGTTQGCHPAWEPRSESQASRKEEGLPEEEPSHVTGREGFPTDAPYPTTLGKDRECQSQSLALKEQNNLKQLEFGLKEAPVQDQGYKTLRLRENCVLSSSPNPFPEISRGEYLYTYDSQITDSEHNSSLVSQQTGSPGKQPGENSDCHRDSSQAIPITELTKSQVQDKPYKCTDCGKSFNHNAHLTVHKRIHTGERPYMCKECGKAFSQNSSLVQHERIHTGDKPYKCAECGKSFCHSTHLTVHRRIHTGEKPYECQDCGRAFNQNSSLGRHKRTHTGEKPYTCSVCGKSFSRTTCLFLHLRTHTEERPYECNHCGKGFRHSSSLAQHQRKHAGEKPFECRQRLIFEQTPALTKHEWTEALGCDPPLSQDERTHRSDRPFKCNQCGKCFIQSSHLIRHQITHTREEQPHGRSRRREQSSSRNSHLVQHQHPNSRKSSAGGAKAGQPESRALALFDIQKIMQEKNPVHVIGVEEPSVGASMLFDIREST |
| 分子量 | 91.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人ZNF8蛋白的研究目前公开的文献较少，以下是基于锌指蛋白家族研究和重组蛋白技术构建的**模拟示例**，供参考(实际文献需通过学术数据库进一步检索)：
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1. **文献名称**：*Structural and functional analysis of recombinant human ZNF8 protein*
**作者**：Chen, X., et al.
**摘要**：本研究克隆并表达了重组人ZNF8蛋白，通过X射线晶体学解析其结构，发现其锌指结构域在DNA结合中起关键作用，揭示了其在调控肿瘤相关基因中的潜在功能。
2. **文献名称**：*ZNF8 knockdown inhibits cell proliferation via transcriptional regulation in hepatocellular carcinoma*
**作者**：Wang, L., & Zhang, Y.
**摘要**：利用重组ZNF8蛋白进行功能实验，证实ZNF8通过结合特定启动子区域激活下游基因表达，促进肝癌细胞增殖，为癌症治疗提供新靶点。
3. **文献名称**：*Expression and purification of recombinant ZNF8 protein for epigenetic studies*
**作者**：Smith, J., et al.
**摘要**：优化大肠杆菌表达系统生产高纯度重组ZNF8蛋白，验证其与组蛋白修饰酶的相互作用，探索其在表观遗传调控中的角色。
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**建议**：
- ZNF8的研究可能集中于锌指蛋白家族(如ZNFs)中，可扩展检索范围(如“锌指蛋白+重组表达”)。
- 尝试关键词：ZNF8 AND (recombinant OR expression OR function)
- 查阅综述类文献，如“锌指蛋白在基因调控中的功能”。
建议通过PubMed、Web of Science等平台获取最新研究。如需具体文献协助，请提供更多背景信息或研究领域。
Recombinant human ZNF8 (Zinc Finger Protein 8) is a genetically engineered protein belonging to the zinc finger transcription factor family, which plays critical roles in DNA binding and transcriptional regulation. Zinc finger proteins are characterized by conserved zinc finger motifs—structural domains stabilized by zinc ions—that enable sequence-specific interactions with nucleic acids. ZNF8 contains multiple C2H2-type zinc finger repeats, typically involved in gene expression modulation, chromatin remodeling, and cellular differentiation processes. While its exact physiological functions remain under investigation, studies suggest ZNF8 may participate in transcriptional repression, cell cycle regulation, and tissue-specific development.
The recombinant form is commonly produced in heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable functional studies. Purification techniques like affinity chromatography ensure high purity for experimental use. Researchers utilize recombinant ZNF8 to investigate its DNA-binding specificity, interaction partners, and role in diseases, including potential links to cancer or developmental disorders. Its recombinant availability facilitates *in vitro* assays (e.g., electrophoretic mobility shift assays) and structural studies to decipher molecular mechanisms.
Interest in ZNF8 also stems from its potential application in gene editing technologies, as engineered zinc finger domains serve as customizable DNA-targeting tools. Ongoing research aims to clarify its endogenous targets and regulatory networks, highlighting its significance in both basic biology and therapeutic innovation.
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