| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMG2L1 |
| Uniprot No | Q9UGU5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-601aa |
| 氨基酸序列 | MAYDDSVKKE DCFDGDHTFE DIGLAAGRSQ REKKRSYKDF LREEEEIAAQ VRNSSKKKLK DSELYFLGTD THKKKRKHSS DDYYYGDISS LESSQKKKKK SSPQSTDTAM DLLKAITSPL AAGSKPSKKT GEKSSGSSSH SESKKEHHRK KVSGSSGELP LEDGGSHKSK KMKPLYVNTE TLTLREPDGL KMKLILSPKE KGSSSVDEES FQYPSQQATV KKSSKKSARD EQGALLLGHE LQSFLKTARK KHKSSSDAHS SPGPEGCGSD ASQFAESHSA NLDLSGLEPI LVESDSSSGG ELEAGELVID DSYREIKKKK KSKKSKKKKD KEKHKEKRHS KSKRSLGLSA VPVGEVTVTS GPPPSIPYAG AAAPPLPLPG LHTDGHSEKK KKKEEKDKER ERGEKPKKKN MSAYQVFCKE YRVTIVADHP GIDFGELSKK LAEVWKQLPE KDKLIWKQKA QYLQHKQNKA EATTVKRKAS SSEGSMKVKA SSVGVLSPQK KSPPTTMLLP ASPAKAPETE PIDVAAHLQL LGESLSLIGH RLQETEGMVA VSGSLSVLLD SIICALGPLA CLTTQLPELN GCPKQVLSNT LDNIAYIMPG L |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMG2L1重组蛋白的参考文献示例(注：部分内容基于模拟文献归纳，建议通过学术数据库核实具体信息)：
1. **"Recombinant expression and functional characterization of human HMG2L1 protein"**
*作者：Zhang Y, et al. (2018)*
摘要：本研究成功在大肠杆菌中表达了重组HMG2L1蛋白，并通过体外实验证实其通过结合DNA调控特定基因的转录活性，为后续功能研究奠定基础。
2. **"Structural insights into HMG2L1-DNA interaction using recombinant protein crystallography"**
*作者：Tanaka K, et al. (2020)*
摘要：利用重组HMG2L1蛋白进行X射线晶体学分析，揭示了其与DNA结合的分子机制，发现其通过特定结构域识别DNA小沟，影响染色质重塑。
3. **"HMG2L1 recombinant protein promotes cellular proliferation in cancer cell lines"**
*作者：Wang L, et al. (2019)*
摘要：通过纯化重组HMG2L1蛋白处理癌细胞，发现其通过激活Wnt/β-catenin信号通路促进细胞增殖，提示其在肿瘤发生中的潜在作用。
4. **"Optimization of HMG2L1 recombinant protein production in mammalian expression systems"**
*作者：Gupta R, et al. (2021)*
摘要：优化哺乳动物细胞表达体系提高HMG2L1重组蛋白产量，并验证其在类器官培养中的生物学活性，为组织工程应用提供新工具。
**注意**：以上文献信息为示例性质，实际研究中请通过PubMed、Web of Science等平台以“HMG2L1 recombinant protein”为关键词检索最新文献，并关注《Biochemical Journal》《FEBS Letters》等期刊相关论文。
HMG2L1 (High Mobility Group Nucleosome-Binding Domain-Containing Protein 2-Like 1) is a member of the HMG-box protein family, characterized by its conserved DNA-binding domain known as the HMG-box. This structural motif enables HMG proteins to interact with DNA, influencing chromatin architecture, transcriptional regulation, and DNA repair. HMG2L1. also referred to as SMARCE1-related protein, shares functional similarities with other HMG proteins but exhibits distinct roles in cellular processes. It is implicated in modulating chromatin remodeling, facilitating transcriptional activation or repression by binding to specific DNA sequences or nucleosomes.
Recombinant HMG2L1 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, DNA-binding specificity, and interactions with other regulatory proteins. Its recombinant form is critical for *in vitro* assays, such as electrophoretic mobility shift assays (EMSAs) or chromatin immunoprecipitation (ChIP) mimicry, to dissect molecular mechanisms. Recent studies suggest HMG2L1’s involvement in embryonic development, stem cell differentiation, and cellular stress responses. Dysregulation of HMG2L1 has been linked to pathologies, including cancers and neurodevelopmental disorders, highlighting its potential as a therapeutic target.
Research on recombinant HMG2L1 also explores its role in viral replication, as some viruses hijack host chromatin remodelers. Despite progress, its full interactome and context-dependent functions remain under investigation. The production of high-purity recombinant HMG2L1 enables structural studies (e.g., crystallography or cryo-EM) to resolve its DNA-binding dynamics, aiding drug design for diseases associated with chromatin dysregulation.
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