| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NMP4 |
| Uniprot No | Q8TF68 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | Q8TF68 |
| 氨基酸序列 | MEESHFNSNPYFWPSIPTVSGQIENTMFINKMKDQLLPEKGCGLAPPHYPTLLTVPASVSLPSGISMDTESKSDQLTPHSQASVTQNITVVPVPSTGLMTAGVSCSQRWRREGSQSRGPGLVITSPSGSLVTTASSAQTFPISAPMIVSALPPGSQALQVVPDLSKKVASTLTEEGGGGGGGGGSVAPKPPRGRKKKRMLESGLPEMNDPYVLSPEDDDDHQKDGKTYRCRMCSLTFYSKSEMQI |
| 预测分子量 | 53.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NMP4重组蛋白的参考文献示例(内容为虚构演示，建议通过学术数据库核实真实文献)：
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1. **文献名称**: *Functional Characterization of Recombinant NMP4 in Transcriptional Regulation*
**作者**: Sullivan, T.P. et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组NMP4蛋白，证实其通过结合特定DNA序列调控靶基因转录，并揭示了其核定位信号的关键作用。
2. **文献名称**: *Structural Insights into NMP4 Recombinant Protein and Its Role in Osteoblast Differentiation*
**作者**: Zhang, L., & Lee, J.C.
**摘要**: 通过X射线晶体学解析了重组NMP4的三维结构，发现其锌指结构域对成骨细胞分化具有调控功能，为骨疾病治疗提供新靶点。
3. **文献名称**: *NMP4 Recombinant Protein Modulates Inflammatory Response via NF-κB Pathway*
**作者**: Hirose, M., et al.
**摘要**: 实验表明重组NMP4通过与NF-κB相互作用抑制炎症因子表达，提示其在自身免疫性疾病中的潜在治疗价值。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词"NMP4 recombinant protein"或"Nuclear Matrix Protein 4 expression"获取最新研究。
**Background of NMP4 Recombinant Protein**
NMP4 (Nuclear Matrix Protein 4), also known as CIST (CIAO1-interacting stromal antigen) or ZNF384. is a transcription regulatory protein encoded by the *ZNF384* gene. It belongs to the zinc finger protein family, characterized by C2H2-type zinc finger motifs that facilitate DNA binding and protein-protein interactions. NMP4 is implicated in regulating gene expression linked to cellular processes such as differentiation, proliferation, and apoptosis. Its role in bone metabolism and oncogenesis has drawn significant research interest.
Structurally, recombinant NMP4 is engineered using expression systems like *E. coli* or mammalian cells, ensuring proper post-translational modifications for functional studies. The recombinant form retains DNA-binding activity and interacts with transcriptional co-regulators, including RUNX family proteins, to modulate osteoblast differentiation and skeletal development. Studies highlight its dual role: NMP4 enhances osteoblast-specific gene __expression (e.g., *ALPL*, *BGLAP*) while suppressing adipogenic pathways, positioning it as a key mediator in bone-fat lineage allocation.
In cancer biology, NMP4 is linked to leukemia and solid tumors. Chromosomal translocations involving *ZNF384* create fusion proteins that drive oncogenic transcriptional programs, particularly in B-cell acute lymphoblastic leukemia (B-ALL). Additionally, NMP4 overexpression in breast and prostate cancers correlates with bone metastasis, potentially through dysregulation of extracellular matrix remodeling genes.
Research on recombinant NMP4 aids in elucidating its molecular mechanisms, including its interplay with signaling pathways (e.g., BMP, Wnt) and epigenetic modifiers. It also serves as a tool for drug screening, aiming to develop therapies for osteoporosis, cancer metastasis, and hematologic malignancies. Despite progress, its context-dependent functions—tumor-suppressive or pro-metastatic—remain under investigation, underscoring the need for further studies to harness its therapeutic potential.
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