Recombinant Human NSMASE protein

中文名： 中性鞘磷脂酶(NSMASE)重组蛋白
别名： NSMASE；Sphingomyelin phosphodiesterase 3

货号: PA1000-8768

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	NSMASE
Uniprot No	O60906
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-423aa
氨基酸序列	MKPNFSLRLRIFNLNCWGIPYLSKHRADRMRRLGDFLNQESFDLALLEEV WSEQDFQYLRQKLSPTYPAAHHFRSGIIGSGLCVFSKHPIQELTQHIYTL NGYPYMIHHGDWFSGKAVGLLVLHLSGMVLNAYVTHLHAEYNRQKDIYLA HRVAQAWELAQFIHHTSKKADVVLLCGDLNMHPEDLGCCLLKEWTGLHDA YLETRDFKGSEEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAVSGFYI SCKSFETTTGFDPHSGTPLSDHEALMATLFVRHSPPQQNPSSTHGPAERS PLMCVLKEAWTELGLGMAQARWWATFASYVIGLGLLLLALLCVLAAGGGA GEAAILLWTPSVGLVLWAGAFYLFHVQEVNGLYRAQAELQHVLGRAREAQ DLGPEPQPALLLGQQEGDRTKEQ
预测分子量	73 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于NSMASE(中性鞘磷脂酶)重组蛋白研究的3篇参考文献概览：

1. **《Recombinant human neutral sphingomyelinase: production, purification, and characterization》**

- 作者：Tomiuk, S., et al.

- 摘要：该研究报道了人源中性鞘磷脂酶(nSMase)的重组表达与纯化方法，利用大肠杆菌系统成功获得高活性重组蛋白，并对其酶学性质(如pH依赖性、金属离子激活)进行了详细表征，为后续功能研究奠定基础。

2. **《Neutral sphingomyelinase-2 deficiency impairs extracellular vesicle biogenesis and exacerbates Alzheimer’s pathology》**

- 作者：Asai, H., et al.

- 摘要：通过重组nSMase2蛋白实验，揭示了该酶在外泌体生成中的关键作用，证明其缺陷会加剧β淀粉样蛋白沉积，为阿尔茨海默病的分子机制提供了新视角。

3. **《Role of neutral sphingomyelinase in cytokine-induced endothelial cell activation》**

- 作者：Clarke, C.J., et al.

- 摘要：研究利用重组nSMase蛋白，证明其在肿瘤坏死因子α(TNF-α)刺激下介导内皮细胞炎症反应，通过鞘脂代谢通路调控血管黏附分子表达，提示其作为炎症疾病的潜在治疗靶点。

注：文献标题与作者为示例性质，具体研究请根据实际需求通过PubMed/Web of Science检索最新论文。重组nSMase研究多聚焦于其在脂代谢、神经退行性疾病及肿瘤领域的分子机制。

背景信息

**Background of NSMASE Recombinant Protein**

Neutral sphingomyelinase (NSMASE) is a key enzyme in sphingolipid metabolism, catalyzing the hydrolysis of sphingomyelin to produce ceramide and phosphocholine. This enzymatic reaction plays a critical role in cellular signaling pathways, influencing processes such as apoptosis, inflammation, and stress responses. NSMASE exists in multiple isoforms, with NSMASE2 (also known as SMPD3) being the most studied due to its involvement in physiological and pathological conditions, including liver fibrosis, cardiovascular diseases, and neurodegenerative disorders.

Recombinant NSMASE proteins are engineered using genetic cloning techniques, where the NSMASE gene is expressed in heterologous systems like *E. coli*, yeast, or mammalian cells. This approach ensures high purity, scalability, and consistent activity, overcoming limitations of native protein extraction from tissues. Recombinant NSMASE retains enzymatic specificity, enabling researchers to study its structure-function relationships, regulatory mechanisms, and interactions with inhibitors or activators.

The development of recombinant NSMASE has advanced drug discovery, particularly in targeting ceramide-mediated pathways. For instance, aberrant NSMASE activity is linked to chronic inflammation and cancer progression, making it a potential therapeutic target. Additionally, recombinant NSMASE serves as a tool for high-throughput screening of small molecules or biologics to modulate its activity.

Overall, recombinant NSMASE proteins provide a versatile platform for both basic research and translational applications, bridging gaps in understanding sphingolipid biology and fostering innovation in treating related diseases.