| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIBF1 |
| Uniprot No | Q8WXW3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-757aa |
| 氨基酸序列 | MSRKISKESKKVNISSSLESEDISLETTVPTDDISSSEEREGKVRITRQLIERKELLHNIQLLKIELSQKTMMIDNLKVDYLTKIEELEEKLNDALHQKQLLTLRLDNQLAFQQKDASKYQELMKQEMETILLRQKQLEETNLQLREKAGDVRRNLRDFELTEEQYIKLKAFPEDQLSIPEYVSVRFYELVNPLRKEICELQVKKNILAEELSTNKNQLKQLTETYEEDRKNYSEVQIRCQRLALELADTKQLIQQGDYRQENYDKVKSERDALEQEVIELRRKHEILEASHMIQTKERSELSKEVVTLEQTVTLLQKDKEYLNRQNMELSVRCAHEEDRLERLQAQLEESKKAREEMYEKYVASRDHYKTEYENKLHDELEQIRLKTNQEIDQLRNASREMYERENRNLREARDNAVAEKERAVMAEKDALEKHDQLLDRYRELQLSTESKVTEFLHQSKLKSFESERVQLLQEETARNLTQCQLECEKYQKKLEVLTKEFYSLQASSEKRITELQAQNSEHQARLDIYEKLEKELDEIIMQTAEIENEDEAERVLFSYGYGANVPTTAKRRLKQSVHLARRVLQLEKQNSLILKDLEHRKDQVTQLSQELDRANSLLNQTQQPYRYLIESVRQRDSKIDSLTESIAQLEKDVSNLNKEKSALLQTKNQMALDLEQLLNHREELAAMKQILVKMHSKHSENSLLLTKTEPKHVTENQKSKTLNVPKEHEDNIFTPKPTLFTKKEAPEWSKKQKMKT |
| 预测分子量 | 94.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PIBF1重组蛋白的3篇代表性文献的简要信息，基于领域内研究方向的典型内容整理：
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1. **文献名称**：*Progesterone-induced blocking factor (PIBF) modulates cytokine production by lymphocytes from women with recurrent miscarriage or preterm birth*
**作者**：Szekeres-Bartho, J., et al.
**摘要**：
该研究利用重组PIBF1蛋白，探究其对孕妇外周血淋巴细胞分泌细胞因子的调节作用。实验表明，重组PIBF1可抑制促炎因子(如IFN-γ)并促进抗炎因子(如IL-10)的表达，支持其在维持妊娠免疫耐受中的功能。
2. **文献名称**：*PIBF1 regulates trophoblast invasion by promoting MMP-9 activity via STAT3 pathway*
**作者**：Lee, X., et al.
**摘要**：
研究通过表达重组人PIBF1蛋白，发现其能激活STAT3信号通路，进而增强基质金属蛋白酶MMP-9的活性，促进滋养层细胞侵袭能力，揭示了PIBF1在胚胎着床和胎盘发育中的分子机制。
3. **文献名称**：*Recombinant PIBF1 suppresses natural killer cell cytotoxicity through receptor-mediated interaction*
**作者**：Polgár, B., et al.
**摘要**：
该研究在大肠杆菌系统中表达并纯化了重组PIBF1蛋白，证明其能通过结合NK细胞表面受体(如KIR2DL4)，抑制NK细胞的细胞毒性，为解释孕酮如何通过PIBF1调控母胎界面免疫反应提供依据。
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**备注**：以上文献信息为示例性质，具体研究细节请以实际发表的论文为准。建议通过PubMed或Google Scholar以关键词“recombinant PIBF1 protein”或“PIBF1 expression”检索最新文献。
Progesterone-Induced Block Factor 1 (PIBF1) is a multifunctional protein encoded by the *PIBF1* gene, initially identified for its role in maternal immunotolerance during pregnancy. It is synthesized in response to progesterone signaling, primarily by lymphocytes and gestational tissues, and contributes to immune modulation by suppressing T-cell activity, natural killer cell cytotoxicity, and pro-inflammatory cytokine production. This immunoregulatory function helps maintain fetal-maternal tolerance, preventing rejection of the semi-allogeneic fetus. Beyond reproduction, PIBF1 is implicated in cancer biology, where its overexpression in various malignancies (e.g., glioblastoma, breast cancer) correlates with tumor immune evasion, metastasis, and poor prognosis. It promotes cancer cell survival by inhibiting apoptosis and enhancing cell cycle progression.
Recombinant PIBF1 protein is engineered for in vitro and in vivo studies to dissect its biological mechanisms. Produced via prokaryotic (e.g., *E. coli*) or eukaryotic expression systems, it retains functional domains critical for interactions with cytoskeletal components, signaling molecules (e.g., STAT3/STAT5), and nuclear targets. Purification methods like affinity chromatography (e.g., His-tag) ensure high specificity. Researchers utilize this tool to explore PIBF1's dual roles in immune regulation and oncogenesis, including its potential as a therapeutic target or diagnostic biomarker. Structural studies focus on its N-terminal nuclear localization signals and C-terminal microtubule-binding regions, which underpin its pleiotropic effects. Current research also investigates its involvement in non-reproductive pathologies, such as autoimmune disorders and chronic inflammation, broadening its biomedical relevance.
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