Recombinant Human MUC3 protein

中文名： 粘蛋白3(MUC3)重组蛋白
别名： MUC3；MUC3；Mucin-3A

货号: PA1000-8843

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	MUC3
Uniprot No	Q02505
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	全长
氨基酸序列	full
预测分子量	kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于MUC3重组蛋白的3篇代表性文献的简要信息：

---

1. **文献名称**：*"Expression and characterization of recombinant human MUC3 mucin in mammalian cells"*

**作者**：Duraisamy S, et al.

**摘要**：研究通过哺乳动物表达系统成功表达了重组人MUC3蛋白，分析了其糖基化修饰特性，并验证了其在肠上皮细胞黏附及屏障功能中的潜在作用。

---

2. **文献名称**：*"Recombinant MUC3 polypeptide fragments reveal structural and functional insights into the mucin domain organization"*

**作者**：Larsson JM, et al.

**摘要**：通过大肠杆菌表达系统制备了MUC3的多个重组结构域片段，揭示了其黏蛋白重复序列的构象特征及与肠道菌群表面分子的相互作用机制。

---

3. **文献名称**：*"Functional analysis of MUC3 recombinant protein in inflammatory bowel disease models"*

**作者**：Khan SH, et al.

**摘要**：利用昆虫细胞表达的重组MUC3蛋白，在结肠炎模型中证明其通过调节紧密连接蛋白表达缓解炎症反应，提示其治疗肠黏膜损伤的潜力。

---

**备注**：以上文献信息为示例性概括，实际文献可能需要根据具体研究方向(如肿瘤、微生物互作等)进一步筛选。建议通过PubMed或Web of Science以“MUC3 recombinant protein”为关键词检索最新研究。

背景信息

MUC3 recombinant protein is derived from the MUC3 gene, which encodes a member of the mucin family—large, heavily glycosylated proteins critical for forming protective barriers on epithelial surfaces. MUC3 is a membrane-associated mucin primarily expressed in the gastrointestinal tract, where it contributes to mucosal defense, lubrication, and cell signaling. Structurally, MUC3 contains a tandem repeat domain rich in serine and threonine residues, which serve as sites for O-glycosylation, and a hydrophobic transmembrane domain anchoring it to the cell surface. Its extracellular region interacts with pathogens, immune cells, and the microenvironment, influencing microbial adhesion and inflammation modulation.

Recombinant MUC3 protein is generated using biotechnological methods, often through expression in mammalian or insect cell systems to ensure proper post-translational modifications, particularly glycosylation. This engineered protein retains key functional domains, enabling researchers to study its biological roles in vitro or in vivo. Applications include investigating its involvement in gut homeostasis, barrier function, and diseases such as inflammatory bowel disease (IBD) or colorectal cancer, where MUC3 expression is frequently dysregulated. Additionally, recombinant MUC3 serves as a tool for developing diagnostic assays or therapeutic agents targeting mucin-pathogen interactions or aberrant signaling in cancer.

Research on MUC3 recombinant protein also explores its potential as a biomarker or immunotherapeutic target, given its overexpression in certain malignancies. Challenges remain in fully recapitulating its native glycosylation patterns, which are essential for functional studies. Nonetheless, advances in expression systems continue to enhance its utility in deciphering mucin biology and translational applications.