Recombinant Human PKP2 protein

中文名： 血小板亲和蛋白2(PKP2)重组蛋白
别名： PKP2；Plakophilin-2

货号: PA1000-8860

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	PKP2
Uniprot No	Q99959
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-881aa
氨基酸序列	MAAPGAPAEYGYIRTVLGQQILGQLDSSSLALPSEAKLKLAGSSGRGGQTVKSLRIQEQVQQTLARKGRSSVGNGNLHRTSSVPEYVYNLHLVENDFVGGRSPVPKTYDMLKAGTTATYEGRWGRGTAQYSSQKSVEERSLRHPLRRLEISPDSSPERAHYTHSDYQYSQRSQAGHTLHHQESRRAALLVPPRYARSEIVGVSRAGTTSRQRHFDTYHRQYQHGSVSDTVFDSIPANPALLTYPRPGTSRSMGNLLEKENYLTAGLTVGQVRPLVPLQPVTQNRASRSSWHQSSFHSTRTLREAGPSVAVDSSGRRAHLTVGQAAAGGSGNLLTERSTFTDSQLGNADMEMTLERAVSMLEADHMLPSRISAAATFIQHECFQKSEARKRVNQLRGILKLLQLLKVQNEDVQRAVCGALRNLVFEDNDNKLEVAELNGVPRLLQVLKQTRDLETKKQITDHTVNLRSRNGWPGAVAHACNPSTLGGQGGRITRSGVRDQPDQHGLLWNLSSNDKLKNLMITEALLTLTENIIIPFSGWPEGDYPKANGLLDFDIFYNVTGCLRNMSSAGADGRKAMRRCDGLIDSLVHYVRGTIADYQPDDKATENCVCILHNLSYQLEAELPEKYSQNIYIQNRNIQTDNNKSIGCFGSRSRKVKEQYQDVPMPEEKSNPKGVEWLWHSIVIRMYLSLIAKSVRNYTQEASLGALQNLTAGSGPMPTSVAQTVVQKESGLQHTRKMLHVGDPSVKKTAISLLRNLSRNLSLQNEIAKETLPDLVSIIPDTVPSTDLLIETTASACYTLNNIIQNSYQNARDLLNTGGIQKIMAISAGDAYASNKASKAASVLLYSLWAHTELHHAYKKAQFKKTDFVNSRTAKAYHSLKD
预测分子量	97,4 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PKP2重组蛋白的模拟参考文献示例(注：以下内容为虚构，仅用于示例)：

1. **标题**: *Recombinant Expression and Structural Analysis of Plakophilin-2 (PKP2) in Cardiomyocytes*

**作者**: Smith A, et al.

**摘要**: 本研究利用大肠杆菌系统成功表达并纯化了PKP2重组蛋白，通过X射线晶体学解析其N端结构域的三维结构，揭示了其与桥粒蛋白desmoplakin结合的分子机制，为心律失常性心肌病突变研究奠定基础。

2. **标题**: *Functional Characterization of PKP2 Mutants Using Recombinant Protein Assays*

**作者**: Lee J, et al.

**摘要**: 通过体外重组PKP2蛋白实验，分析了ARVC患者中常见的PKP2基因突变(如R735X)对蛋白稳定性和细胞粘附功能的影响，发现突变导致PKP2与desmoglein-2的结合能力显著下降。

3. **标题**: *PKP2 Recombinant Protein-Based Screening for Cardiac Disease Therapeutics*

**作者**: Garcia R, et al.

**摘要**: 开发了一种基于重组PKP2蛋白的高通量筛选平台，用于筛选增强PKP2与桥粒蛋白相互作用的化合物，初步发现小分子X可部分恢复突变PKP2的稳定性。

4. **标题**: *In Vitro Reconstitution of Desmosomal Complexes with Recombinant PKP2*

**作者**: Chen H, et al.

**摘要**: 利用重组PKP2蛋白与其它桥粒成分(如desmocollin、plakoglobin)在体外重构功能复合体，证明PKP2在维持心肌细胞间机械连接中的核心作用，并揭示了钙离子浓度对其组装的影响。

(注：以上文献为模拟生成，实际引用需以真实出版物为准。)

背景信息

Plakophilin-2 (PKP2) is a crucial component of desmosomes, specialized intercellular junctions that maintain tissue integrity in mechanically stressed organs like the heart and skin. As a member of the armadillo protein family, PKP2 contains nine armadillo repeats that mediate protein-protein interactions. It serves as a scaffold, linking desmosomal cadherins (e.g., desmoglein-2 and desmocollin-2) to the intermediate filament cytoskeleton via interactions with desmoplakin. Beyond structural roles, PKP2 participates in signaling pathways regulating cell adhesion, proliferation, and differentiation.

Mutations in the PKP2 gene are strongly associated with arrhythmogenic cardiomyopathy (ACM), a genetic disorder characterized by fibrofatty myocardial replacement and life-threatening ventricular arrhythmias. Over 20% of ACM cases involve PKP2 mutations, making it the most frequently mutated gene in this disease. These mutations often disrupt desmosome assembly, leading to impaired cardiomyocyte adhesion and aberrant Wnt/β-catenin signaling.

Recombinant PKP2 protein is produced using expression systems (e.g., E. coli, mammalian cells) for functional studies. It enables researchers to investigate mutation-specific effects on protein stability, binding affinities, and post-translational modifications. Purified PKP2 fragments are used in crystallography studies to resolve its 3D structure and interaction interfaces. In drug discovery, recombinant PKP2 serves as a target for compounds aiming to stabilize desmosomal complexes or modulate downstream signaling pathways. Recent studies also explore its potential role in regulating connexin 43 gap junctions, linking structural defects to electrical abnormalities in ACM.