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Recombinant Human EAAT2 protein

  • 中文名: 兴奋性氨基酸转运蛋白2(EAAT2)重组蛋白
  • 别    名: EAAT2;EAAT2;GLT1;;Excitatory amino acid transporter 2
货号: PA1000-8927
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点EAAT2
Uniprot No P43004
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-574aa
氨基酸序列MASTEGANNMPKQVEVRMHDSHLGSEEPKHRHLGLRLCDKLGKNLLLTLTVFGVILGAVCGGLLRLASPIHPDVVMLIAFPGDILMRMLKMLILPLIISSLITGLSGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVLAIHPGNPKLKKQLGPGKKNDEVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPPDEEANATSAVVSLLNETVTEVPEETKMVIKKGLEFKDGMNVLGLIGFFIAFGIAMGKMGDQAKLMVDFFNILNEIVMKLVIMIMWYSPLGIACLICGKIIAIKDLEVVARQLGMYMVTVIIGLIIHGGIFLPLIYFVVTRKNPFSFFAGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGVVLDGGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTIDSQHRVHEDIEMTKTQSIYDDMKNHRESNSNQCVYAAHNSVIVDECKVTLAANGKSADCSVEEEPWKREK
预测分子量62,1 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于EAAT2重组蛋白的3篇参考文献示例(注:文献名为假设示例,非真实存在):

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1. **文献名称**:*High-yield expression and functional characterization of recombinant human EAAT2 in mammalian cell lines*

**作者**:Smith J, Doe R, Lee A

**摘要**:该研究报道了在HEK293细胞中通过质粒转染高效表达重组人源EAAT2蛋白的方法,并利用亲和层析技术纯化获得高纯度蛋白。功能实验表明,重组EAAT2具有谷氨酸摄取活性,且对选择性抑制剂DHK敏感,证实其生理相关性。

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2. **文献名称**:*Structural analysis of EAAT2 using a recombinant baculovirus-insect cell expression system*

**作者**:Chen X, Wang Y, Zhang L

**摘要**:作者利用杆状病毒-昆虫细胞系统表达重组EAAT2蛋白,通过冷冻电镜解析其三维结构。研究发现,EAAT2的跨膜结构域在谷氨酸转运过程中发生构象变化,为设计靶向神经退行性疾病的药物提供了结构基础。

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3. **文献名称**:*Recombinant EAAT2 mitigates glutamate toxicity in a rodent model of ALS*

**作者**:Brown K, Garcia M, Patel T

**摘要**:该研究通过腺病毒载体在ALS模型小鼠中过表达重组EAAT2蛋白,结果显示其显著降低脊髓中谷氨酸浓度,延缓神经元退变并改善运动功能,提示重组EAAT2在神经保护治疗中的潜力。

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**备注**:以上文献为示例,实际研究中建议通过PubMed或Google Scholar检索关键词(如“EAAT2 recombinant protein”、“GLT-1 expression”)获取真实文献。

背景信息

EAAT2 (Excitatory Amino Acid Transporter 2), also known as GLT-1 in rodents, is a sodium-dependent glutamate transporter predominantly expressed in astrocytes within the central nervous system. It plays a critical role in maintaining extracellular glutamate homeostasis by rapidly clearing synaptic glutamate, thereby preventing excitotoxicity—a process implicated in neurodegenerative disorders like Alzheimer’s disease, amyotrophic lateral sclerosis (ALS), and epilepsy. Dysregulation of EAAT2 is linked to impaired glutamate uptake, leading to neuronal damage and disease progression.

Recombinant EAAT2 proteins are engineered using expression systems (e.g., bacterial, mammalian, or insect cells) to study its structure, function, and regulatory mechanisms. These proteins enable researchers to investigate ligand interactions, transport kinetics, and the impact of mutations or pharmacological modulators. For instance, structural studies using recombinant EAAT2 have revealed insights into its trimeric configuration and the role of specific domains in substrate binding and ion coupling.

Pharmaceutically, recombinant EAAT2 serves as a tool for drug screening to identify compounds that enhance its expression or activity, offering therapeutic potential. Beta-lactam antibiotics like ceftriaxone, known to upregulate EAAT2. were discovered using such approaches. Challenges remain in ensuring proper post-translational modifications and membrane localization of recombinant proteins, which affect functional accuracy.

Current research focuses on gene therapy and small molecules to modulate EAAT2. aiming to restore glutamate balance in neurological diseases. Its dual role as a neuroprotectant and a biomarker underscores its translational significance, driving ongoing efforts to harness EAAT2 for diagnostics and targeted therapies.

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