Recombinant Human MTERF protein

中文名： 线粒体转录终止因子(MTERF)重组蛋白
别名： MTERF；MTERF；Transcription termination factor 1, mitochondrial

货号: PA1000-9075

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	MTERF
Uniprot No	Q49AM1
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	36-385aa
氨基酸序列	TTDKQ SSKENTRTVE KLYKCSVDIR KIRRLKGWVL LEDETYVEEI ANILQELGAD ETAVASILER CPEAIVCSPT AVNTQRKLWQ LVCKNEEELI KLIEQFPESF FTIKDQENQK LNVQFFQELG LKNVVISRLL TAAPNVFHNP VEKNKQMVRI LQESYLDVGG SEANMKVWLL KLLSQNPFIL LNSPTAIKET LEFLQEQGFT SFEILQLLSK LKGFLFQLCP RSIQNSISFS KNAFKCTDHD LKQLVLKCPA LLYYSVPVLE ERMQGLLREG ISIAQIRETP MVLELTPQIV QYRIRKLNSS GYRIKDGHLA NLNGSKKEFE ANFGKIQAKK VRPLFNPVAP LNVEE
预测分子量	kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇关于MTERF重组蛋白的关键文献摘要：

1. **文献名称**：MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome

**作者**：Cámara Y, et al.

**摘要**：该研究发现MTERF4通过与NSUN4甲基转移酶结合，调控线粒体核糖体的组装和翻译功能，利用重组蛋白技术解析了其相互作用机制及对线粒体蛋白合成的影响。

2. **文献名称**：Structure of MTERF in complex with mitochondrial RNA

**作者**：Yakubovskaya E, et al.

**摘要**：通过重组表达人源MTERF1蛋白并进行晶体结构分析，揭示了其如何结合线粒体tRNA并调控RNA转录终止的分子基础。

3. **文献名称**：MTERF2 promotes human mtDNA transcription by recruiting the transcription machinery components

**作者**：Linder T, et al.

**摘要**：研究利用重组MTERF2蛋白进行体外生化实验，证明其通过招募线粒体RNA聚合酶等转录机器，直接激活线粒体DNA的转录过程。

背景信息

MTERF (Mitochondrial Transcription Termination Factor) recombinant proteins are derived from a family of evolutionarily conserved proteins primarily involved in mitochondrial gene regulation. Initially identified in humans, MTERF proteins play critical roles in transcription, replication, and translation within mitochondria, organelles responsible for cellular energy production. The first member, MTERF1. was discovered as a DNA-binding protein that terminates transcription of the mitochondrial heavy strand, ensuring proper synthesis of rRNA and mRNA. Subsequent studies revealed additional paralogs (MTERF2-4) across species, each with distinct but overlapping functions in mitochondrial DNA (mtDNA) maintenance, ribosome biogenesis, and stress responses.

Structurally, MTERF proteins contain characteristic helical repeats that facilitate nucleic acid binding. Recombinant MTERF proteins are typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) for functional and structural studies. These engineered proteins enable researchers to dissect molecular mechanisms, such as how MTERF1 induces transcription termination by bending mtDNA or how MTERF4 stabilizes mitochondrial ribosomes. Dysregulation of MTERF proteins has been linked to mitochondrial disorders, neurodegenerative diseases, and cancer, highlighting their biomedical relevance. For example, MTERF3 knockout in mice causes embryonic lethality due to mtDNA depletion, while MTERF4 mutations impair mitochondrial translation, leading to metabolic defects. Recombinant variants with targeted mutations or tags (e.g., GFP, His-tags) further aid in exploring interactions with mtDNA, RNA, or partner proteins. Current research focuses on resolving their precise roles in mitochondrial homeostasis and potential therapeutic applications.