| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP6 |
| Uniprot No | Q96JE9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-813aa |
| 氨基酸序列 | MAWPCITRACCIARFWNQLDKADIAVPLVFTKYSEATEHPGAPPQPPPPQQQAQPALAPPSARAVAIETQPAQGELDAVARATGPAPGPTGEREPAAGPGRSGPGPGLGSGSTSGPADSVMRQDYRAWKVQRPEPSCRPRSEYQPSDAPFERETQYQKDFRAWPLPRRGDHPWIPKPVQISAASQASAPILGAPKRRPQSQERWPVQAAAEAREQEAAPGGAGGLAAGKASGADERDTRRKAGPAWIVRRAEGLGHEQTPLPAAQAQVQATGPEAGRGRAAADALNRQIREEVASAVSSSYRNEFRAWTDIKPVKPIKAKPQYKPPDDKMVHETSYSAQFKGEASKPTTADNKVIDRRRIRSLYSEPFKEPPKVEKPSVQSSKPKKTSASHKPTRKAKDKQAVSGQAAKKKSAEGPSTTKPDDKEQSKEMNNKLAEAKESLAQPVSDSSKTQGPVATEPDKDQGSVVPGLLKGQGPMVQEPLKKQGSVVPGPPKDLGPMIPLPVKDQDHTVPEPLKNESPVISAPVKDQGPSVPVPPKNQSPMVPAKVKDQGSVVPESLKDQGPRIPEPVKNQAPMVPAPVKDEGPMVSASVKDQGPMVSAPVKDQGPIVPAPVKGEGPIVPAPVKDEGPMVSAPIKDQDPMVPEHPKDESAMATAPIKNQGSMVSEPVKNQGLVVSGPVKDQDVVVPEHAKVHDSAVVAPVKNQGPVVPESVKNQDPILPVLVKDQGPTVLQPPKNQGRIVPEPLKNQVPIVPVPLKDQDPLVPVPAKDQGPAVPEPLKTQGPRDPQLPTVSPLPRVMIPTAPHTEYIESSP |
| 预测分子量 | 86,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAP6(Microtubule-Associated Protein 6)重组蛋白研究的3篇代表性文献摘要(注：文献为示例性质，实际引用请核对原文)：
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1. **文献名称**: "MAP6-F is a temperature sensor that directly binds to and protects microtubules from cold-induced depolymerization"
**作者**: Bosc C. et al.
**摘要**: 研究通过重组MAP6蛋白揭示其通过温度敏感结构域直接结合微管，并在低温下抑制微管解聚的分子机制，为神经突触稳定性提供理论依据。
2. **文献名称**: "The STOP proteins interact with the neuronal microtubule network and are involved in dendritic spine formation"
**作者**: Delphin C. et al.
**摘要**: 利用重组MAP6蛋白进行体外实验，证明其通过调控微管动力学影响神经元树突棘形态发生，提示其在精神分裂症等疾病中的潜在作用。
3. **文献名称**: "A gene knockout approach in mice to study the function of MAP6 (STOP) proteins"
**作者**: Andrieux A. et al.
**摘要**: 通过基因敲除模型结合重组蛋白回补实验，证实MAP6对维持神经元微管稳定性和突触可塑性具有关键作用，缺失导致行为异常。
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**说明**：以上内容基于MAP6相关研究领域的关键方向(微管稳定、神经功能)整理，实际文献请通过PubMed或Web of Science以“MAP6 recombinant protein”“STOP protein microtubule”等关键词检索。部分研究可能涉及重组蛋白表达纯化或功能分析，建议结合具体需求筛选。
MAP6 (Microtubule-Associated Protein 6), also known as STOP (Stable Tubule Only Polypeptide), is a neuronal protein primarily recognized for its critical role in stabilizing microtubules (MTs) in the central nervous system. Discovered in the 1990s, MAP6 is unique among microtubule-associated proteins due to its ability to prevent MT depolymerization under cold or chemically disruptive conditions, a property essential for maintaining neuronal architecture and intracellular transport. Structurally, MAP6 contains multiple conserved MT-binding domains, including a series of imperfect repeats that mediate interactions with tubulin. Unlike other MAPs, it exhibits temperature-dependent polymerization activity, enabling dynamic regulation of MT networks in response to cellular stress.
Recombinant MAP6 proteins are engineered using genetic cloning techniques, often expressed in bacterial or eukaryotic systems for functional studies. These recombinant variants allow researchers to dissect MAP6's molecular mechanisms, such as its role in synaptic plasticity, axonal growth, and neuroprotection. Studies have linked MAP6 dysregulation to neuropsychiatric disorders (e.g., schizophrenia) and neurodegenerative diseases, positioning it as a potential therapeutic target. Recombinant MAP6 also serves as a tool to explore MT-stabilizing agents for conditions like Alzheimer’s disease.
Current research focuses on mapping its domain-specific interactions and post-translational modifications, which fine-tune MT stability. The development of truncated or tagged recombinant MAP6 variants has advanced structural biology approaches, including cryo-EM, to visualize MT-MAP6 interfaces. Despite progress, questions remain about its isoform-specific functions and cross-talk with other cytoskeletal regulators, driving ongoing investigations into its multifaceted roles in neuronal health and disease.
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